Netprimer
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Netprimer
NetPrimer is a gratis web-based tool used for analysing Primer (molecular biology), primers used in Polymerase chain reaction, PCR to amplify a DNA sequence. The software predicts the melting temperature of the primers using the nearest neighbor thermodynamic algorithm. The accurate prediction of the melting temperature (Tm) is one of the most important factors that governs the success of a PCR reaction. NetPrimer also analyzes the thermodynamically important secondary structures such as stem loop, hairpins, self and cross primer dimer, dimers, runs and repeats. These structures significantly affect the primer efficiency and therefore the success of a PCR reaction. NetPrimer can be used to determine the best primer pairs for a given set of experimental conditions. The program assigns a rating to each primer analyzed. The rating is based on the proximity of the thermodynamic parameters to their ideal scores. In addition to the primer quality, its molecular weight and Nucleic acid ...
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6 May 2002
6 (six) is the natural number following 5 and preceding 7. It is a composite number and the smallest perfect number. In mathematics Six is the smallest positive integer which is neither a square number nor a prime number; it is the second smallest composite number, behind 4; its proper divisors are , and . Since 6 equals the sum of its proper divisors, it is a perfect number; 6 is the smallest of the perfect numbers. It is also the smallest Granville number, or \mathcal-perfect number. As a perfect number: *6 is related to the Mersenne prime 3, since . (The next perfect number is 28 (number), 28.) *6 is the only even perfect number that is not the sum of successive odd cubes. *6 is the root of the 6-aliquot tree, and is itself the aliquot sum of only one other number; the square number, . Six is the only number that is both the sum and the product of three consecutive positive numbers. Unrelated to 6's being a perfect number, a Golomb ruler of length 6 is a "perfect ruler". Si ...
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