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MIAME
Minimum information about a microarray experiment (MIAME) is a standard created by the FGED Society for reporting microarray experiments. MIAME is intended to specify all the information necessary to interpret the results of the experiment unambiguously and to potentially reproduce the experiment. While the standard defines the content required for compliant reports, it does not specify the format in which this data should be presented. MIAME describes the minimum information required to ensure that microarray data can be easily interpreted and that results derived from its analysis can be independently verified. There are a number of file formats used to represent this data, as well as both public and subscription-based repositories for such experiments. Additionally, software exists to aid the preparation of MIAME-compliant reports. MIAME revolves around six key components: raw data, normalized data, sample annotations, experimental design The design of experiments (DO ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
FGED Society
The Functional GEnomics Data Society (FGED) (formerly known as the MGED Society) was a non-profit, volunteer-run international organization of biologists, computer scientists, and data analysts that aims to facilitate biological and biomedical discovery through data integration. The approach of FGED was to promote the sharing of basic research data generated primarily via high-throughput technologies that generate large data sets within the domain of functional genomics. Members of the FGED Society worked with other organizations to support the effective sharing and reproducibility of functional genomics data; facilitate the creation of standards and software tools that leverage the standards; and promote the sharing of high quality, well annotated data within the life sciences and biomedical communities. Founded in 1999 as the "Microarray Gene Expression Data (MGED) Society", this organization changed its name to the "Functional Genomics Data Society" in 2010 to reflect the ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Microarray
A microarray is a multiplex lab-on-a-chip. Its purpose is to simultaneously detect the expression of thousands of genes from a sample (e.g. from a tissue). It is a two-dimensional array on a solid substrate—usually a glass slide or silicon thin-film cell—that assays (tests) large amounts of biological material using high-throughput screening miniaturized, multiplexed and parallel processing and detection methods. The concept and methodology of microarrays was first introduced and illustrated in antibody microarrays (also referred to as antibody matrix) by Tse Wen Chang in 1983 in a scientific publication and a series of patents. The " gene chip" industry started to grow significantly after the 1995 '' Science Magazine'' article by the Ron Davis and Pat Brown labs at Stanford University. With the establishment of companies, such as Affymetrix, Agilent, Applied Microarrays, Arrayjet, Illumina, and others, the technology of DNA microarrays has become the most sophistic ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Experiment
An experiment is a procedure carried out to support or refute a hypothesis, or determine the efficacy or likelihood of something previously untried. Experiments provide insight into cause-and-effect by demonstrating what outcome occurs when a particular factor is manipulated. Experiments vary greatly in goal and scale but always rely on repeatable procedure and logical analysis of the results. There also exist natural experimental studies. A child may carry out basic experiments to understand how things fall to the ground, while teams of scientists may take years of systematic investigation to advance their understanding of a phenomenon. Experiments and other types of hands-on activities are very important to student learning in the science classroom. Experiments can raise test scores and help a student become more engaged and interested in the material they are learning, especially when used over time. Experiments can vary from personal and informal natural comparisons ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
File Format
A file format is a standard way that information is encoded for storage in a computer file. It specifies how bits are used to encode information in a digital storage medium. File formats may be either proprietary or free. Some file formats are designed for very particular types of data: PNG files, for example, store bitmapped images using lossless data compression. Other file formats, however, are designed for storage of several different types of data: the Ogg format can act as a container for different types of multimedia including any combination of audio and video, with or without text (such as subtitles), and metadata. A text file can contain any stream of characters, including possible control characters, and is encoded in one of various character encoding schemes. Some file formats, such as HTML, scalable vector graphics, and the source code of computer software are text files with defined syntaxes that allow them to be used for specific purposes. Speci ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Raw Data
Raw data, also known as primary data, are ''data'' (e.g., numbers, instrument readings, figures, etc.) collected from a source. In the context of examinations, the raw data might be described as a raw score (after test scores). If a scientist sets up a computerized thermometer which records the temperature of a chemical mixture in a test tube every minute, the list of temperature readings for every minute, as printed out on a spreadsheet or viewed on a computer screen are "raw data". Raw data have not been subjected to processing, "cleaning" by researchers to remove outliers, obvious instrument reading errors or data entry errors, or any analysis (e.g., determining central tendency aspects such as the average or median result). As well, raw data have not been subject to any other manipulation by a software program or a human researcher, analyst or technician. They are also referred to as ''primary'' data. Raw data is a relative term (see data), because even once raw data have ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Normalised Data
Database normalization or database normalisation (see spelling differences) is the process of structuring a relational database in accordance with a series of so-called normal forms in order to reduce data redundancy and improve data integrity. It was first proposed by British computer scientist Edgar F. Codd as part of his relational model. Normalization entails organizing the columns (attributes) and tables (relations) of a database to ensure that their dependencies are properly enforced by database integrity constraints. It is accomplished by applying some formal rules either by a process of ''synthesis'' (creating a new database design) or ''decomposition'' (improving an existing database design). Objectives A basic objective of the first normal form defined by Codd in 1970 was to permit data to be queried and manipulated using a "universal data sub-language" grounded in first-order logic. An example of such a language is SQL, though it is one that Codd regarded as seri ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Experimental Design
The design of experiments (DOE, DOX, or experimental design) is the design of any task that aims to describe and explain the variation of information under conditions that are hypothesized to reflect the variation. The term is generally associated with experiments in which the design introduces conditions that directly affect the variation, but may also refer to the design of quasi-experiments, in which natural conditions that influence the variation are selected for observation. In its simplest form, an experiment aims at predicting the outcome by introducing a change of the preconditions, which is represented by one or more independent variables, also referred to as "input variables" or "predictor variables." The change in one or more independent variables is generally hypothesized to result in a change in one or more dependent variables, also referred to as "output variables" or "response variables." The experimental design may also identify control variables that must b ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Biochemistry Detection Methods
Biochemistry or biological chemistry is the study of chemical processes within and relating to living organisms. A sub-discipline of both chemistry and biology, biochemistry may be divided into three fields: structural biology, enzymology and metabolism. Over the last decades of the 20th century, biochemistry has become successful at explaining living processes through these three disciplines. Almost all areas of the life sciences are being uncovered and developed through biochemical methodology and research. Voet (2005), p. 3. Biochemistry focuses on understanding the chemical basis which allows biological molecules to give rise to the processes that occur within living cells and between cells, Karp (2009), p. 2. in turn relating greatly to the understanding of tissues and organs, as well as organism structure and function.Miller (2012). p. 62. Biochemistry is closely related to molecular biology, which is the study of the molecular mechanisms of biological phenomena.Ast ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Genetics Techniques
Genetics is the study of genes, genetic variation, and heredity in organisms.Hartl D, Jones E (2005) It is an important branch in biology because heredity is vital to organisms' evolution. Gregor Mendel, a Moravian Augustinian friar working in the 19th century in Brno, was the first to study genetics scientifically. Mendel studied "trait inheritance", patterns in the way traits are handed down from parents to offspring over time. He observed that organisms (pea plants) inherit traits by way of discrete "units of inheritance". This term, still used today, is a somewhat ambiguous definition of what is referred to as a gene. Trait inheritance and molecular inheritance mechanisms of genes are still primary principles of genetics in the 21st century, but modern genetics has expanded to study the function and behavior of genes. Gene structure and function, variation, and distribution are studied within the context of the cell, the organism (e.g. dominance), and within the contex ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
