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Melzer's Reagent
Melzer's reagent (also known as Melzer's iodine reagent, Melzer's solution or informally as Melzer's) is a chemical reagent used by mycologists to assist with the identification of fungi, and by phytopathologists for fungi that are plant pathogens. Composition Melzer's reagent is an aqueous solution of chloral hydrate, potassium iodide, and iodine. Depending on the formulation, it consists of approximately 2.50-3.75% potassium iodide and 0.75–1.25% iodine, with the remainder of the solution being 50% water and 50% chloral hydrate. Melzer's is toxic to humans if ingested due to the presence of iodine and chloral hydrate. Due to the legal status of chloral hydrate, Melzer's reagent is difficult to obtain in the United States. In response to difficulties obtaining chloral hydrate, scientists at Rutgers formulated Visikol (compatible with Lugol's iodine) as a replacement. In 2019, research showed that Visikol behaves differently to Melzer’s reagent in several key situations, notin ...
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Auriscalpium Vulgare 137307
''Auriscalpium'' is a genus of mushrooms type (biology), typifying the family (biology), family Auriscalpiaceae. Etymology ''Auriscalpium'' is a compound of the Latin language, Latin, ''auris'', "ear"; and ''scalpo'', "I scratch", generally meaning ear pick. The term was originally applied as a specific name (botany), specific epithet by Carl Linnaeus, Linnaeus in 1753, viz. ''Hydnum auriscalpium'' and changed in 1821 to ''vulgare'' when Samuel Frederick Gray, S.F. Gray recognized the cone-inhabiting fungus as a new genus, named after its type species, ''Auriscalpium vulgare''. Tautonyms, such as ''"Auriscalpium auriscalpium"'' are illegitimate under the International Code of Botanical Nomenclature. Taxonomy The genus includes the following species: * ''Auriscalpium andinum, A. andinum'' * ''Auriscalpium barbatum, A. barbatum'' * ''Auriscalpium dissectum, A. dissectum'' * ''Auriscalpium gilbertsonii, A. gilbertsonii'' * ''Auriscalpium luteolum, A. luteolum'' * ''Auriscalpium ...
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Lugol's Solution
Lugol's iodine, also known as aqueous iodine and strong iodine solution, is a solution of potassium iodide with iodine in water. It is a medication and disinfectant used for a number of purposes. Taken by mouth it is used to treat thyrotoxicosis until surgery can be carried out, protect the thyroid gland from radioactive iodine, and to treat iodine deficiency. When applied to the cervix it is used to help in screening for cervical cancer. As a disinfectant it may be applied to small wounds such as a needle stick injury. A small amount may also be used for emergency disinfection of drinking water. Side effects may include allergic reactions, headache, vomiting, and conjunctivitis. Long term use may result in trouble sleeping and depression. It should not typically be used during pregnancy or breastfeeding. Lugol's iodine is a liquid made up of two parts potassium iodide for every one part elemental iodine in water. Lugol's iodine was first made in 1829 by the French physicia ...
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Mycology
Mycology is the branch of biology concerned with the study of fungi, including their genetic and biochemical properties, their taxonomy and their use to humans, including as a source for tinder, traditional medicine, food, and entheogens, as well as their dangers, such as toxicity or infection. A biologist specializing in mycology is called a mycologist. Mycology branches into the field of phytopathology, the study of plant diseases, and the two disciplines remain closely related because the vast majority of plant pathogens are fungi. Overview Historically, mycology was a branch of botany because, although fungi are evolutionarily more closely related to animals than to plants, this was not recognized until a few decades ago. Pioneer mycologists included Elias Magnus Fries, Christian Hendrik Persoon, Anton de Bary, Elizabeth Eaton Morse, and Lewis David von Schweinitz. Beatrix Potter, author of ''The Tale of Peter Rabbit'', also made significant contributions to the fiel ...
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Spore
In biology, a spore is a unit of sexual or asexual reproduction that may be adapted for dispersal and for survival, often for extended periods of time, in unfavourable conditions. Spores form part of the life cycles of many plants, algae, fungi and protozoa. Bacterial spores are not part of a sexual cycle, but are resistant structures used for survival under unfavourable conditions. Myxozoan spores release amoeboid infectious germs ("amoebulae") into their hosts for parasitic infection, but also reproduce within the hosts through the pairing of two nuclei within the plasmodium, which develops from the amoebula. In plants, spores are usually haploid and unicellular and are produced by meiosis in the sporangium of a diploid sporophyte. Under favourable conditions the spore can develop into a new organism using mitotic division, producing a multicellular gametophyte, which eventually goes on to produce gametes. Two gametes fuse to form a zygote which develops into a new s ...
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Russula
''Russula'' is a very large genus composed of around 750 worldwide species of ectomycorrhizal mushrooms. They are typically common, fairly large, and brightly colored – making them one of the most recognizable genera among mycologists and mushroom collectors. Their distinguishing characteristics include usually brightly coloured caps, a white to dark yellow spore print, brittle, attached gill (mushroom), gills, an absence of latex, and absence of partial veil or universal veil, volva tissue on the stem. Microscopically, the genus is characterised by the amyloid ornamented spores and flesh (trama) composed of spherocysts. Members of the related genus ''Lactarius (fungus), Lactarius'' have similar characteristics but emit a milky latex when their gills are broken. The genus was described by Christian Hendrik Persoon in 1796. Taxonomy Christian Hendrik Persoon first circumscription (taxonomy), circumscribed the genus ''Russula'' in his 1796 work ''Observationes Mycologicae'', and c ...
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Arthur Meyer (botanist)
Arthur Meyer (1850–1922) was a German botanist, cell biologist, and pharmacognosist. Meyer is known for his pioneering work describing the structure of chloroplasts (which Meyer called "autoplasts") and other plastids. He was the first to name and describe the chlorophyll-containing structures in chloroplasts known as grana. Meyer spent his academic career at the University of Marburg, where he was a member of the school's Marburg Circle, an interdisciplinary biological discussion group centered on Emil von Behring.Grundmann K. 2001"Emil von Behring: The founder of serum therapy"(web page). '' Nobelprize.org''. References Works * ''Wissenschaftliche Drogenkunde : ein illustriertes Lehrbuch der Pharmakognosie u. eine wissenschaftliche Anleitung zur eingehenden botanischen Untersuchung pflanzlicher Drogen für Apotheker. Band 1'' . Gaertner, Berlin 189Digital editionby the University and State Library Düsseldorf * ''Wissenschaftliche Drogenkunde : ein illustriertes Lehrbuch d ...
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Václav Melzer
Václav Melzer (26 August 1878 – 1 May 1968) was a Czech teacher and mycologist, who was part of a group of Czech teachers who became mycologists at the beginning of the 20th century that also included figures such as Jindřich Kučera, Rudolf Veselý, and František Tyttl. A substantial part of his life was spent living and working in Domažlice. Melzer's scientific work was devoted mainly to ''Russula'', in which he published the first Czech monograph of this basidiomycete genus. His discoveries in this genus included the ''Russula sardonia'' variety ''mellini'', described in 1927, and '' Russula helodes'' in 1929, based on collections from the Soběslav area. The mycologist Josef Velenovský named the bolete species '' Boletus melzeri'' in his honor in 1922, which was initially collected by Melzer in 1920 at Čechtice. Other taxa named after Melzer include the species '' Lentinus melzeri'', '' Russula melzeri'', and ''Russula lilacea'' var. ''melzeriana'' (now '' Russula mel ...
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Ammonium Hydroxide
Ammonia solution, also known as ammonia water, ammonium hydroxide, ammoniacal liquor, ammonia liquor, aqua ammonia, aqueous ammonia, or (inaccurately) ammonia, is a solution of ammonia in water. It can be denoted by the symbols NH3(aq). Although the name ammonium hydroxide suggests an alkali with chemical formula, composition , it is actually impossible to isolate samples of NH4OH. The ions and OH− do not account for a significant fraction of the total amount of ammonia except in extremely dilute solutions. Basicity of ammonia in water In aqueous solution, ammonia deprotonation, deprotonates a small fraction of the water to give ammonium and hydroxide according to the following chemical equilibrium, equilibrium: : NH3 + H2O NH4+ + OH−. In a 1 Molar concentration, M ammonia solution, about 0.42% of the ammonia is converted to ammonium, equivalent to pH = 11.63 because [NH4+] = 0.0042 M, [OH−] = 0.0042 M, [NH3] = 0.9958 M, and pH = 14 + log10[OH ...
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Basidiomycota
Basidiomycota () is one of two large divisions that, together with the Ascomycota, constitute the subkingdom Dikarya (often referred to as the "higher fungi") within the kingdom Fungi. Members are known as basidiomycetes. More specifically, Basidiomycota includes these groups: mushrooms, puffballs, stinkhorns, bracket fungi, other polypores, jelly fungi, boletes, chanterelles, earth stars, smuts, bunts, rusts, mirror yeasts, and ''Cryptococcus'', the human pathogenic yeast. Basidiomycota are filamentous fungi composed of hyphae (except for basidiomycota-yeast) and reproduce sexually via the formation of specialized club-shaped end cells called basidia that normally bear external meiospores (usually four). These specialized spores are called basidiospores. However, some Basidiomycota are obligate asexual reproducers. Basidiomycota that reproduce asexually (discussed below) can typically be recognized as members of this division by gross similarity to others, by the form ...
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Iodine Test
Iodine is a chemical element with the symbol I and atomic number 53. The heaviest of the stable halogens, it exists as a semi-lustrous, non-metallic solid at standard conditions that melts to form a deep violet liquid at , and boils to a violet gas at . The element was discovered by the French chemist Bernard Courtois in 1811 and was named two years later by Joseph Louis Gay-Lussac, after the Ancient Greek 'violet-coloured'. Iodine occurs in many oxidation states, including iodide (I−), iodate (), and the various periodate anions. It is the least abundant of the stable halogens, being the sixty-first most abundant element. As the heaviest essential mineral nutrient, iodine is required for the synthesis of thyroid hormones. Iodine deficiency affects about two billion people and is the leading preventable cause of intellectual disabilities. The dominant producers of iodine today are Chile and Japan. Due to its high atomic number and ease of attachment to organic compounds, i ...
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Solubility
In chemistry, solubility is the ability of a substance, the solute, to form a solution with another substance, the solvent. Insolubility is the opposite property, the inability of the solute to form such a solution. The extent of the solubility of a substance in a specific solvent is generally measured as the concentration of the solute in a saturated solution, one in which no more solute can be dissolved. At this point, the two substances are said to be at the solubility equilibrium. For some solutes and solvents, there may be no such limit, in which case the two substances are said to be " miscible in all proportions" (or just "miscible"). The solute can be a solid, a liquid, or a gas, while the solvent is usually solid or liquid. Both may be pure substances, or may themselves be solutions. Gases are always miscible in all proportions, except in very extreme situations,J. de Swaan Arons and G. A. M. Diepen (1966): "Gas—Gas Equilibria". ''Journal of Chemical Physics'', ...
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Clearing Agent
Optical sectioning is the process by which a suitably designed microscope can produce clear images of focal planes deep within a thick sample. This is used to reduce the need for thin sectioning using instruments such as the microtome. Many different techniques for optical sectioning are used and several microscopy techniques are specifically designed to improve the quality of optical sectioning. Good optical sectioning, often referred to as good depth or z resolution, is popular in modern microscopy as it allows the three-dimensional reconstruction of a sample from images captured at different focal planes. Optical sectioning in traditional light microscopes In an ideal microscope, only light from the focal plane would be allowed to reach the detector (typically an observer or a CCD) producing a clear image of the plane of the sample the microscope is focused on. Unfortunately a microscope is not this specific and light from sources outside the focal plane also reaches the dete ...
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