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MALDI
In mass spectrometry, matrix-assisted laser desorption/ionization (MALDI) is an ionization technique that uses a laser energy absorbing matrix to create ions from large molecules with minimal fragmentation. It has been applied to the analysis of biomolecules ( biopolymers such as DNA, proteins, peptides and carbohydrates) and various organic molecules (such as polymers, dendrimers and other macromolecules), which tend to be fragile and fragment when ionized by more conventional ionization methods. It is similar in character to electrospray ionization (ESI) in that both techniques are relatively soft (low fragmentation) ways of obtaining ions of large molecules in the gas phase, though MALDI typically produces far fewer multi-charged ions. MALDI methodology is a three-step process. First, the sample is mixed with a suitable matrix material and applied to a metal plate. Second, a pulsed laser irradiates the sample, triggering ablation and desorption of the sample and matrix materi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
MALDITOF
In mass spectrometry, matrix-assisted laser desorption/ionization (MALDI) is an ionization technique that uses a laser energy absorbing matrix to create ions from large molecules with minimal fragmentation. It has been applied to the analysis of biomolecules ( biopolymers such as DNA, proteins, peptides and carbohydrates) and various organic molecules (such as polymers, dendrimers and other macromolecules), which tend to be fragile and fragment when ionized by more conventional ionization methods. It is similar in character to electrospray ionization (ESI) in that both techniques are relatively soft (low fragmentation) ways of obtaining ions of large molecules in the gas phase, though MALDI typically produces far fewer multi-charged ions. MALDI methodology is a three-step process. First, the sample is mixed with a suitable matrix material and applied to a metal plate. Second, a pulsed laser irradiates the sample, triggering ablation and desorption of the sample and matrix mater ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Franz Hillenkamp
Franz Hillenkamp (March 18, 1936 – August 22, 2014) was a German scientist known for his development of the laser microprobe mass analyzer and, with Michael Karas, matrix-assisted laser desorption/ionization (MALDI). Early life and education Franz Hillenkamp was born in 1936 in Essen, Germany. He attended high school in Lünen, graduating in 1955. He received a M.S. degree in electrical engineering from Purdue University in 1961. He received a Ph.D. (Dr.-Ing.) from the Technische Universität München in 1966 with a thesis entitled “An Absolutely Calibrated Calorimeter for the Measurement of Pulsed Laser Radiation.” Academic career Hillenkamp was a professor at Goethe University Frankfurt in Frankfurt from 1982 to 1986. In 1986, he became a professor on the Medical Faculty of the University of Münster where he remained until his retirement in 2001. Laser microprobe In 1973, Hillenkamp developed a high performance laser microprobe mass spectrometer with a spatial resolution ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mass Spectrometry
Mass spectrometry (MS) is an analytical technique that is used to measure the mass-to-charge ratio of ions. The results are presented as a ''mass spectrum'', a plot of intensity as a function of the mass-to-charge ratio. Mass spectrometry is used in many different fields and is applied to pure samples as well as complex mixtures. A mass spectrum is a type of plot of the ion signal as a function of the mass-to-charge ratio. These spectra are used to determine the elemental or isotopic signature of a sample, the masses of particles and of molecules, and to elucidate the chemical identity or structure of molecules and other chemical compounds. In a typical MS procedure, a sample, which may be solid, liquid, or gaseous, is ionized, for example by bombarding it with a beam of electrons. This may cause some of the sample's molecules to break up into positively charged fragments or simply become positively charged without fragmenting. These ions (fragments) are then separated accordin ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Koichi Tanaka
is a Japanese electrical engineer who shared the Nobel Prize in Chemistry in 2002 for developing a novel method for mass spectrometric analyses of biological macromolecules with John Bennett Fenn and Kurt Wüthrich (the latter for work in NMR spectroscopy). Early life and education Tanaka was born and raised in Toyama, Japan, his biological mother died one month after he was born. Tanaka graduated from Tohoku University with a bachelor's degree in electrical engineering in 1983, afterward he joined Shimadzu Corporation, where he engaged in the development of mass spectrometers. Soft laser desorption For mass spectrometry analyses of a macromolecule, such as a protein, the analyte must be ionized and vaporized by laser irradiation. The problem is that the direct irradiation of an intense laser pulse on a macromolecule causes cleavage of the analyte into tiny fragments and the loss of its structure. In February 1985, Tanaka found that by using a mixture of ultra fine metal pow ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Electrospray Ionization
Electrospray ionization (ESI) is a technique used in mass spectrometry to produce ions using an electrospray in which a high voltage is applied to a liquid to create an aerosol. It is especially useful in producing ions from macromolecules because it overcomes the propensity of these molecules to fragment when ionized. ESI is different from other ionization processes (e.g. matrix-assisted laser desorption/ionization (MALDI)) since it may produce multiple-charged ions, effectively extending the mass range of the analyser to accommodate the kDa-MDa orders of magnitude observed in proteins and their associated polypeptide fragments. Mass spectrometry using ESI is called electrospray ionization mass spectrometry (ESI-MS) or, less commonly, electrospray mass spectrometry (ES-MS). ESI is a so-called 'soft ionization' technique, since there is very little fragmentation. This can be advantageous in the sense that the molecular ion (or more accurately a pseudo molecular ion) is almost alw ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Peptide
Peptides (, ) are short chains of amino acids linked by peptide bonds. Long chains of amino acids are called proteins. Chains of fewer than twenty amino acids are called oligopeptides, and include dipeptides, tripeptides, and tetrapeptides. A polypeptide is a longer, continuous, unbranched peptide chain. Hence, peptides fall under the broad chemical classes of biological polymers and oligomers, alongside nucleic acids, oligosaccharides, polysaccharides, and others. A polypeptide that contains more than approximately 50 amino acids is known as a protein. Proteins consist of one or more polypeptides arranged in a biologically functional way, often bound to ligands such as coenzymes and cofactors, or to another protein or other macromolecule such as DNA or RNA, or to complex macromolecular assemblies. Amino acids that have been incorporated into peptides are termed residues. A water molecule is released during formation of each amide bond.. All peptides except cyclic pep ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Michael Karas
Michael Karas (born 1952) is a German physical chemistry scientist and Professor, known for his researches on matrix-assisted laser desorption/ionization (MALDI), a technique in mass spectrometry. Michael Karas studied Chemistry at the University of Bonn, where he obtained a PhD in the field of physical chemistry in 1982. From 1983 to 1986, he was part of the Hillenkamp research group in the Institut für Biophysik at Johann Wolfgang Goethe University Frankfurt am Main. In 1987, he followed Hillenkamp at Münster and both formed a group in the Faculty of Medicine at University of Münster The University of Münster (german: Westfälische Wilhelms-Universität Münster, WWU) is a public research university located in the city of Münster, North Rhine-Westphalia in Germany. With more than 43,000 students and over 120 fields of stud .... He returned to Frankfurt in 1995 as a full professor for Instrumental Analytical Chemistry. Awards * 1997: Recipient of the John B. Fenn Aw ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Glycerol
Glycerol (), also called glycerine in British English and glycerin in American English, is a simple triol compound. It is a colorless, odorless, viscous liquid that is sweet-tasting and non-toxic. The glycerol backbone is found in lipids known as glycerides. Because it has antimicrobial and antiviral properties, it is widely used in wound and burn treatments approved by the U.S. Food and Drug Administration. Conversely, it is also used as a bacterial culture medium. It can be used as an effective marker to measure liver disease. It is also widely used as a sweetener in the food industry and as a humectant in pharmaceutical formulations. Because of its three hydroxyl groups, glycerol is miscible with water and is hygroscopic in nature. Structure Although achiral, glycerol is prochiral with respect to reactions of one of the two primary alcohols. Thus, in substituted derivatives, the stereospecific numbering labels the molecule with a "sn-" prefix before the stem name of the m ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cobalt
Cobalt is a chemical element with the symbol Co and atomic number 27. As with nickel, cobalt is found in the Earth's crust only in a chemically combined form, save for small deposits found in alloys of natural meteoric iron. The free element, produced by reductive smelting, is a hard, lustrous, silver-gray metal. Cobalt-based blue pigments ( cobalt blue) have been used since ancient times for jewelry and paints, and to impart a distinctive blue tint to glass, but the color was for a long time thought to be due to the known metal bismuth. Miners had long used the name ''kobold ore'' (German for ''goblin ore'') for some of the blue-pigment-producing minerals; they were so named because they were poor in known metals, and gave poisonous arsenic-containing fumes when smelted. In 1735, such ores were found to be reducible to a new metal (the first discovered since ancient times), and this was ultimately named for the ''kobold''. Today, some cobalt is produced specifically from one of ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
International Journal Of Mass Spectrometry And Ion Processes
The ''International Journal of Mass Spectrometry'' is a monthly peer-reviewed scientific journal covering all aspects of mass spectrometry, including instrumentation and applications in biology, chemistry, geology, and physics. It was established in 1968 as the ''International Journal of Mass Spectrometry and Ion Physics'' and was renamed ''International Journal of Mass Spectrometry and Ion Processes'' in 1983, before obtaining its current title in 1998. It is published by Elsevier and the editors-in-chief are Julia Laskin (Purdue University) and Zheng Ouyang (Tsinghua University). Abstracting and indexing The journal is abstracted and indexed in: *Chemical Abstracts Service *Current Contents/Physical, Chemical & Earth Sciences * EBSCO databases * Embase *Food Science and Technology Abstracts *FRANCIS * Inspec *PASCAL *Science Citation Index Expanded *Scopus According to the ''Journal Citation Reports'', the journal has a 2020 impact factor The impact factor (IF) or journal impa ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Nitrogen Laser
A nitrogen laser is a gas laser operating in the ultraviolet rangeC. S. Willett, ''Introduction to Gas Lasers: Population Inversion Mechanisms'' (Pergamon, New York,1974). (typically 337.1 nm) using molecular nitrogen as its gain medium, pumped by an electrical discharge. The wall-plug efficiency of the nitrogen laser is low, typically 0.1% or less, though nitrogen lasers with efficiency of up to 3% have been reported in the literature. The wall-plug efficiency is the product of the following three efficiencies: * electrical: TEA laser * gain medium: This is the same for all nitrogen lasers and thus has to be at least 3% ** inversion by electron impact is 10 to 1 due to Franck–Condon principle ** energy lost in the lower laser level: 40% * optical: More stimulated emission than spontaneous emission Gain medium The gain medium is nitrogen molecules in the gas phase. The nitrogen laser is a three-level laser. In contrast to more typical four-level lasers, the upper laser l ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Dalton (unit)
The dalton or unified atomic mass unit (symbols: Da or u) is a non-SI unit of mass widely used in physics and chemistry. It is defined as of the mass of an unbound neutral atom of carbon-12 in its nuclear and electronic ground state and at rest. The atomic mass constant, denoted ''m''u, is defined identically, giving . This unit is commonly used in physics and chemistry to express the mass of atomic-scale objects, such as atoms, molecules, and elementary particles, both for discrete instances and multiple types of ensemble averages. For example, an atom of helium-4 has a mass of . This is an intrinsic property of the isotope and all helium-4 atoms have the same mass. Acetylsalicylic acid (aspirin), , has an average mass of approximately . However, there are no acetylsalicylic acid molecules with this mass. The two most common masses of individual acetylsalicylic acid molecules are , having the most common isotopes, and , in which one carbon is carbon-13. The molecular mass ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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