Lima Bean Agar
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Lima Bean Agar
Lima bean agar (LBA) is an agar medium which is used to cultivate the spores of ''Phytophthora sojae''. ''Phytophthora sojae'' causes soybean blight (Phytophthora root rot of soybeans). Soybean blight can affect the output and quality of soybeans seriously. The spore of ''Phytophthora sojae'' is difficult to culture in potato dextrose agar Potato dextrose agar (BAM Media M127
from the U.S.
; it is generally cultured by lima bean agar and carrot agar. Lima bean agar can cultivate, separate, reproduce and conserve many types of ''Phytophthora sojae'' spores, but it is not suitable for cultivating the pathogen of
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Agar
Agar ( or ), or agar-agar, is a jelly-like substance consisting of polysaccharides obtained from the cell walls of some species of red algae, primarily from ogonori (''Gracilaria'') and "tengusa" (''Gelidiaceae''). As found in nature, agar is a mixture of two components, the linear polysaccharide agarose and a heterogeneous mixture of smaller molecules called agaropectin. It forms the supporting structure in the cell walls of certain species of algae and is released on boiling. These algae are known as agarophytes, belonging to the Rhodophyta (red algae) phylum. The processing of food-grade agar removes the agaropectin, and the commercial product is essentially pure agarose. Agar has been used as an ingredient in desserts throughout Asia and also as a solid substrate to contain culture media for microbiological work. Agar can be used as a laxative; an appetite suppressant; a vegan substitute for gelatin; a thickener for soups; in fruit preserves, ice cream, and other desser ...
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Phytophthora Sojae
''Phytophthora sojae'' is an oomycete and a soil-borne plant pathogen that causes stem and root rot of soybean. This is a prevalent disease in most soybean growing regions, and a major cause of crop loss. In wet conditions the pathogen produces zoospores that move in water and are attracted to soybean roots. Zoospores can attach to roots, germinate, and infect the plant tissues. Diseased roots develop lesions that may spread up the stem and eventually kill the entire plant. ''Phytophthora sojae'' also produces oospores that can remain dormant in the soil over the winter, or longer, and germinate when conditions are favourable. Oospores may also be spread by animals or machinery. ''Phytophthora sojae'' is a diploid organism with a genome size of 95 Mbp (Millions of base pairs). The natural chemical farinomalein (a metabolite from entomopathogenic fungus '' Paecilomyces farinosus''Sastia P. Putri, Hiroshi Kinoshita, Fumio Ihara, Yasuhiro Igarashi and Takuya Nihira. ''Farinomalein, ...
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Soybean
The soybean, soy bean, or soya bean (''Glycine max'') is a species of legume native to East Asia, widely grown for its edible bean, which has numerous uses. Traditional unfermented food uses of soybeans include soy milk, from which tofu and tofu skin are made. Fermented soy foods include soy sauce, fermented bean paste, nattō, and tempeh. Fat-free (defatted) soybean meal is a significant and cheap source of protein for animal feeds and many packaged meals. For example, soybean products, such as textured vegetable protein (TVP), are ingredients in many meat and dairy substitutes. Soybeans contain significant amounts of phytic acid, dietary minerals and B vitamins. Soy vegetable oil, used in food and industrial applications, is another product of processing the soybean crop. Soybean is the most important protein source for feed farm animals (that in turn yields animal protein for human consumption). Etymology The word "soy" originated as a corruption of the Cantonese or ...
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Phytophthora
''Phytophthora'' (from Greek language, Greek (''phytón''), "plant" and (), "destruction"; "the plant-destroyer") is a genus of plant-damaging oomycetes (water molds), whose member species are capable of causing enormous economic losses on crops worldwide, as well as environmental degradation, environmental damage in natural ecosystems. As well as impacting large scale agriculture, ''Phytophthora'' is a nuisance to garden and indoor plant hobbyists as well as bonsai artists. The cell wall of ''Phytophthora'' is made up of cellulose. The genus was first described by Anton de Bary, Heinrich Anton de Bary in 1875. Approximately 170 species have been described, although 100–500 undiscovered ''Phytophthora'' species are estimated to exist. Pathogenicity ''Phytophthora'' species, spp. are mostly pathogens of dicotyledons, and many are relatively host-specific parasites. ''Phytophthora cinnamomi'', though, infects thousands of species ranging from club mosses, ferns, cycads, coni ...
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Potato Dextrose Agar
Potato dextrose agar (BAM Media M127
from the U.S.
) and potato dextrose broth are common microbiological growth media made from and . Potato dextrose agar (abbreviated "PDA") is the most widely used mediu ...
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Carrot Agar
Carrot medium is a kind of growth medium made from a mixture of agar and carrot powder. Carrot medium is used to cultivate the spore of Phytophthora sojae. Phytophthora sojae causes soybean blight (Phytophthora root rot of soybeans). Soybean blight can affect the output and quality of soybeans seriously. The spore of phytophthora sojae is difficult to culture in potato dextrose agar; it is generally cultured by V8 medium and lima bean agar Lima bean agar (LBA) is an agar medium which is used to cultivate the spores of ''Phytophthora sojae''. ''Phytophthora sojae'' causes soybean blight (Phytophthora root rot of soybeans). Soybean blight can affect the output and quality of soybeans ... at home and abroad. Carrot medium can cultivate, separate, reproduce and conserve many kinds spore of phytophthora sojae, but it is not suitable for cultivating the pathogen of potato late blight. References * Sinclair, J.B.; Dhingra, O.D. (1995). Basic Plant Pathology Methods. Taylor & Fra ...
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Phytophthora Infestans
''Phytophthora infestans'' is an oomycete or water mold, a fungus-like microorganism that causes the serious potato and tomato disease known as late blight or potato blight. Early blight, caused by ''Alternaria solani'', is also often called "potato blight". Late blight was a major culprit in the 1840s European, the 1845–1852 Irish, and the 1846 Highland potato famines. The organism can also infect some other members of the Solanaceae. The pathogen is favored by moist, cool environments: sporulation is optimal at in water-saturated or nearly saturated environments, and zoospore production is favored at temperatures below . Lesion growth rates are typically optimal at a slightly warmer temperature range of . Etymology The genus name ''Phytophthora'' comes from the Greek –(), meaning : "plant" – plus the Greek (), meaning : "decay, ruin, perish". The species name ''infestans'' is the present participle of the Latin verb , meaning : "attacking, destroying", from which we ...
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Cell Culture Media
A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation or small plants like the moss ''Physcomitrella patens''. Different types of media are used for growing different types of cells. The two major types of growth media are those used for cell culture, which use specific cell types derived from plants or animals, and those used for microbiological culture, which are used for growing microorganisms such as bacteria or fungi. The most common growth media for microorganisms are nutrient broths and agar plates; specialized media are sometimes required for microorganism and cell culture growth. Some organisms, termed fastidious organisms, require specialized environments due to complex nutritional requirements. Viruses, for example, are obligate intracellular parasites and require a growth medium containing living cells. Types The most common growth media fo ...
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