Lepiota Cristatanea
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Lepiota Cristatanea
''Lepiota cristatanea'' is a species of agaric fungus in the family Agaricaceae. Described as new to science in 2011, it is found in China. The mushroom is similar to the widespread species ''Lepiota cristata'' but can be distinguished by its smaller spores. Taxonomy The species was described in 2001 from collections made in Kunming, China. Due to the close physical similarity between the species, it has often been misidentified as ''Lepiota cristata'', but genetic evidence demonstrates that the two species are clearly distinct. Description The cap is initially roughly bell-shaped to conical, later flattening as the cap matures, and eventually reaches a diameter of . The cap surface features a brownish to orange-brown center that breaks up into small scales concentrically distributed. The gills are fairly crowded, free from attachment to the stipe, and white. There are shorter gills (lamellulae) interspersed between them. The cylindrical, hollow stipe measures long by t ...
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Agaric
An agaric () is a type of fungus fruiting body characterized by the presence of a pileus (cap) that is clearly differentiated from the stipe (stalk), with lamellae (gills) on the underside of the pileus. In the UK, agarics are called "mushrooms" or "toadstools". In North America they are typically called "gilled mushrooms". "Agaric" can also refer to a basidiomycete species characterized by an agaric-type fruiting body. Archaically, agaric meant 'tree-fungus' (after Latin ''agaricum''); however, that changed with the Linnaean interpretation in 1753 when Linnaeus used the generic name ''Agaricus'' for gilled mushrooms. Most species of agaricus belong to the order Agaricales in the subphylum Agaricomycotina. The exceptions, where agarics have evolved independently, feature largely in the orders Russulales, Boletales, Hymenochaetales, and several other groups of basidiomycetes. Old systems of classification placed all agarics in the Agaricales and some (mostly older) sources use ...
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Staining
Staining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently used in histology (microscopic study of biological tissues), in cytology (microscopic study of cells), and in the medical fields of histopathology, hematology, and cytopathology that focus on the study and diagnoses of diseases at the microscopic level. Stains may be used to define biological tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells), or organelles within individual cells. In biochemistry, it involves adding a class-specific ( DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis. Light microscopes are used for viewin ...
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Fungi Described In 2011
A fungus ( : fungi or funguses) is any member of the group of eukaryotic organisms that includes microorganisms such as yeasts and molds, as well as the more familiar mushrooms. These organisms are classified as a kingdom, separately from the other eukaryotic kingdoms, which by one traditional classification include Plantae, Animalia, Protozoa, and Chromista. A characteristic that places fungi in a different kingdom from plants, bacteria, and some protists is chitin in their cell walls. Fungi, like animals, are heterotrophs; they acquire their food by absorbing dissolved molecules, typically by secreting digestive enzymes into their environment. Fungi do not photosynthesize. Growth is their means of mobility, except for spores (a few of which are flagellated), which may travel through the air or water. Fungi are the principal decomposers in ecological systems. These and other differences place fungi in a single group of related organisms, named the ''Eumycota'' (''true fun ...
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Lepiota
''Lepiota'' is a genus of gilled mushrooms in the family Agaricaceae. All ''Lepiota'' species are ground-dwelling saprotrophs with a preference for rich, calcareous soils. Basidiocarps (fruit bodies) are agaricoid with whitish spores, typically with scaly caps and a ring on the stipe. Around 400 species of ''Lepiota'' are currently recognized worldwide. Many species are poisonous, some lethally so. Taxonomy History ''Agaricus'' section ''Lepiota'' was originally published in 1797 by South African-born mycologist Christian Hendrik Persoon. It was subsequently raised to the rank of genus by Samuel Frederick Gray. As originally conceived, the genus was a mix of agarics with rings on their stems, including species now placed in ''Armillaria'', ''Cortinarius'', and ''Pholiota''. In 1822, however, the influential Swedish mycologist Elias Magnus Fries restricted ''Lepiota'' to white-spored, ringed agarics. Based on macro- and micromorphology, later authors gradually refined the gener ...
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List Of Lepiota Species
The following is an incomplete list of species in the agaric genus ''Lepiota''. The genus has a widespread distribution, and contains about 400 species. Some species, such as ''L. aspera'', have now been separated off into the newer genus ''Echinoderma''. *'' Lepiota abruptibulba'' Murrill – Cuba *'' Lepiota acerina'' Peck – New York *'' Lepiota acicularis'' Velen. – Czech Republic *'' Lepiota adusta'' (E. Horak) E. Horak – New Zealand *'' Lepiota aeruginea'' Murrill – Florida *'' Lepiota affinis'' Velen. – *'' Lepiota alba'' Beeli – Congo *'' Lepiota albiceps'' Pat. & Gaillard – Venezuela *'' Lepiota albida'' Massee – Singapore *'' Lepiota albocitrina'' Pat. – Tonkin *'' Lepiota albofibrillosa'' Cleland – Australia *'' Lepiota albosericea'' Henn. – Germany *'' Lepiota albosquamosa'' Rick – Brazil *'' Lepiota allenae'' Peck – Massachusetts *'' Lepiota alliciens'' (Berk.) Sacc. – India *'' Lepiota alluviina'' (Peck) Morgan – New York *'' Lepi ...
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Humus
In classical soil science, humus is the dark organic matter in soil that is formed by the decomposition of plant and animal matter. It is a kind of soil organic matter. It is rich in nutrients and retains moisture in the soil. Humus is the Latin word for "earth" or "ground". In agriculture, "humus" sometimes also is used to describe mature or natural compost extracted from a woodland or other spontaneous source for use as a soil conditioner. It is also used to describe a topsoil horizon that contains organic matter (''humus type'', ''humus form'', or ''humus profile''). Humus has many nutrients that improve the health of soil, nitrogen being the most important. The ratio of carbon to nitrogen (C:N) of humus commonly ranges between eight and fifteen with the median being about twelve. It also significantly affects the bulk density of soil. Humus is amorphous and lacks the "cellular cake structure characteristic of plants, micro-organisms or animals". Description The primary ...
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Yunnan Province
Yunnan , () is a landlocked province in the southwest of the People's Republic of China. The province spans approximately and has a population of 48.3 million (as of 2018). The capital of the province is Kunming. The province borders the Chinese provinces of Guizhou, Sichuan, autonomous regions of Guangxi, and Tibet Autonomous Region, Tibet as well as Southeast Asian countries: Vietnam, Laos, and Myanmar. Yunnan is China's fourth least developed province based on disposable income per capita in 2014. Yunnan is situated in a mountainous area, with high elevations in the northwest and low elevations in the southeast. Most of the population lives in the eastern part of the province. In the west, the altitude can vary from the mountain peaks to river valleys by as much as . Yunnan is rich in natural resources and has the largest diversity of plant life in China. Of the approximately 30,000 species of Vascular plant, higher plants in China, Yunnan has perhaps 17,000 or more. Yun ...
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Clamp Connection
A clamp connection is a hook-like structure formed by growing hyphal cells of certain fungi. It is a characteristic feature of Basidiomycetes fungi. It is created to ensure that each cell, or segment of hypha separated by septa (cross walls), receives a set of differing nuclei, which are obtained through mating of hyphae of differing sexual types. It is used to maintain genetic variation within the hypha much like the mechanisms found in crozier (hook) during sexual reproduction. Formation Clamp connections are formed by the terminal hypha during elongation. Before the clamp connection is formed this terminal segment contains two nuclei. Once the terminal segment is long enough it begins to form the clamp connection. At the same time, each nucleus undergoes mitotic division to produce two daughter nuclei. As the clamp continues to develop it uptakes one of the daughter (green circle) nuclei and separates it from its sister nucleus. While this is occurring the remaining nuclei ...
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Hypha
A hypha (; ) is a long, branching, filamentous structure of a fungus, oomycete, or actinobacterium. In most fungi, hyphae are the main mode of vegetative growth, and are collectively called a mycelium. Structure A hypha consists of one or more cells surrounded by a tubular cell wall. In most fungi, hyphae are divided into cells by internal cross-walls called "septa" (singular septum). Septa are usually perforated by pores large enough for ribosomes, mitochondria, and sometimes nuclei to flow between cells. The major structural polymer in fungal cell walls is typically chitin, in contrast to plants and oomycetes that have cellulosic cell walls. Some fungi have aseptate hyphae, meaning their hyphae are not partitioned by septa. Hyphae have an average diameter of 4–6 µm. Growth Hyphae grow at their tips. During tip growth, cell walls are extended by the external assembly and polymerization of cell wall components, and the internal production of new cell membrane. The S ...
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Cap Cuticle
The pileipellis is the uppermost layer of hyphae in the pileus of a fungal fruit body. It covers the trama, the fleshy tissue of the fruit body. The pileipellis is more or less synonymous with the cuticle, but the cuticle generally describes this layer as a macroscopic feature, while pileipellis refers to this structure as a microscopic layer. Pileipellis type is an important character in the identification of fungi. Pileipellis types include the cutis, trichoderm, epithelium, and hymeniderm types. Types Cutis A cutis is a type of pileipellis characterized by hyphae that are repent, that is, that run parallel to the pileus surface. In an ixocutis, the hyphae are gelatinous. Trichoderm In a trichoderm, the outermost hyphae emerge roughly parallel, like hairs, perpendicular to the cap surface. The prefix "tricho-" comes from a Greek word for "hair". In an ixotrichodermium, the outermost hyphae are gelatinous. Epithelium An epithelium is a pileipellis consisting of rounded ce ...
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Cystidia
A cystidium (plural cystidia) is a relatively large cell found on the sporocarp of a basidiomycete (for example, on the surface of a mushroom gill), often between clusters of basidia. Since cystidia have highly varied and distinct shapes that are often unique to a particular species or genus, they are a useful micromorphological characteristic in the identification of basidiomycetes. In general, the adaptive significance of cystidia is not well understood. Classification of cystidia By position Cystidia may occur on the edge of a lamella (or analogous hymenophoral structure) (cheilocystidia), on the face of a lamella (pleurocystidia), on the surface of the cap (dermatocystidia or pileocystidia), on the margin of the cap (circumcystidia) or on the stipe (caulocystidia). Especially the pleurocystidia and cheilocystidia are important for identification within many genera. Sometimes the cheilocystidia give the gill edge a distinct colour which is visible to the naked eye or wit ...
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Brilliant Cresyl Blue
Brilliant cresyl blue is a supravital stain used for counting reticulocytes. It is classified as an oxazine dye Oxazines are heterocyclic compounds containing one oxygen and one nitrogen atom in a doubly unsaturated six-membered ring. Isomers exist depending on the relative position of the heteroatoms and relative position of the double bonds. By extensi .... N95 dust masks, eye shields, and gloves must all be worn when handling the chemical. References {{heterocyclic-stub Chlorides Zinc compounds Oxazine dyes Phenoxazines ...
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