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LFB Stain
Luxol fast blue stain, abbreviated LFB stain or simply LFB, is a commonly used staining, stain to observe myelin under light microscopy, created by Heinrich Klüver and Elizabeth Barrera in 1953. LFB is commonly used to detect demyelination in the central nervous system (CNS), but cannot discern myelination in the peripheral nervous system. Procedure Luxol fast blue is a copper phthalocyanine dye that is soluble in alcohol and is attracted to bases found in the lipoproteins of the myelin sheath. Under the stain, myelin fibers appear blue, neuropil appears pink, and nerve cells appear purple. Tissues sections are treated over an extended period of time (usually overnight) and then differentiated with a lithium carbonate solution. Combination methods The combination of LFB with a variety of common staining methods provides the most useful and reliable method for the demonstration of pathological processes in the CNS. It is often combined with H&E stain (hematoxylin and eosin), which ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Pons - Very High Mag
The pons (from Latin , "bridge") is part of the brainstem that in humans and other bipeds lies inferior to the midbrain, superior to the medulla oblongata and anterior to the cerebellum. The pons is also called the pons Varolii ("bridge of Varolius"), after the Italian anatomist and surgeon Costanzo Varolio (1543–75). This region of the brainstem includes neural pathways and nerve tract, tracts that conduct signals from the brain down to the cerebellum and medulla, and tracts that carry the sensory signals up into the thalamus.Saladin Kenneth S.(2007) Anatomy & physiology the unity of form and function. Dubuque, IA: McGraw-Hill Structure The pons is in the brainstem situated between the midbrain and the medulla oblongata, and in front of the cerebellum. A separating groove between the pons and the medulla is the inferior pontine sulcus. The superior pontine sulcus separates the pons from the midbrain. The pons can be broadly divided into two parts: the basilar part of the pon ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Nerve Cells
A neuron, neurone, or nerve cell is an electrically excitable cell that communicates with other cells via specialized connections called synapses. The neuron is the main component of nervous tissue in all animals except sponges and placozoa. Non-animals like plants and fungi do not have nerve cells. Neurons are typically classified into three types based on their function. Sensory neurons respond to stimuli such as touch, sound, or light that affect the cells of the sensory organs, and they send signals to the spinal cord or brain. Motor neurons receive signals from the brain and spinal cord to control everything from muscle contractions to glandular output. Interneurons connect neurons to other neurons within the same region of the brain or spinal cord. When multiple neurons are connected together, they form what is called a neural circuit. A typical neuron consists of a cell body (soma), dendrites, and a single axon. The soma is a compact structure, and the axon and dendrite ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Staining
Staining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently used in histology (microscopic study of biological tissues), in cytology (microscopic study of cells), and in the medical fields of histopathology, hematology, and cytopathology that focus on the study and diagnoses of diseases at the microscopic level. Stains may be used to define biological tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells), or organelles within individual cells. In biochemistry, it involves adding a class-specific ( DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis. Light microscopes are used for viewin ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bielschowsky Stain
The Bielschowsky technique is a silver staining method used in histochemistry for the visualization of nerve fibers, including multipolar interneurons in the cerebellum. The method is attributed to German neurologist and neurohistologist Max Bielschowsky (1869–1940), who made improvements over the previous method developed by Ramon y Cajal (1852–1934). See also * Immunohistochemistry * Luxol fast blue stain * Neurofilament Neurofilaments (NF) are classed as type IV intermediate filaments found in the cytoplasm of neurons. They are protein polymers measuring 10 nm in diameter and many micrometers in length. Together with microtubules (~25 nm) and mi ... References {{Stains Staining Histochemistry Histology ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Traumatic Brain Injury
A traumatic brain injury (TBI), also known as an intracranial injury, is an injury to the brain caused by an external force. TBI can be classified based on severity (ranging from mild traumatic brain injury TBI/concussionto severe traumatic brain injury), mechanism ( closed or penetrating head injury), or other features (e.g., occurring in a specific location or over a widespread area). Head injury is a broader category that may involve damage to other structures such as the scalp and skull. TBI can result in physical, cognitive, social, emotional and behavioral symptoms, and outcomes can range from complete recovery to permanent disability or death. Causes include falls, vehicle collisions and violence. Brain trauma occurs as a consequence of a sudden acceleration or deceleration within the cranium or by a complex combination of both movement and sudden impact. In addition to the damage caused at the moment of injury, a variety of events following the injury may result in ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Holmes Silver Nitrate
Holmes may refer to: Name * Holmes (surname) * Holmes (given name) * Baron Holmes, noble title created twice in the Peerage of Ireland * Chris Holmes, Baron Holmes of Richmond (born 1971), British former swimmer and life peer Places In the United States * Holmes, California, an unincorporated community * Holmes, Iowa, an unincorporated community * Holmes, Kentucky, an unincorporated community * Holmes, Pennsylvania, an unincorporated community * Holmes, a hamlet within Pawling (town), New York * Holmes Township, Michigan * Holmes City Township, Minnesota * Holmes Township, Crawford County, Ohio * Holmes County, Florida * Holmes County, Mississippi * Holmes County, Ohio * Mount Holmes, Yellowstone National Park, Wyoming * Fort Holmes, Mackinac Island, Michigan * Holmes Island (Indiana), an island and community * Holmes Island (Washington), an island * Holmes Reservation, a conservation parcel in Plymouth, Massachusetts In Antarctica * Holmes Summit * Holmes Glacier * Hol ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Oil Red O
Oil Red O (Solvent Red 27, Sudan Red 5B, C.I. 26125, C26H24N4O) is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. It has the appearance of a red powder with an absorbance maxima at 518 nanometers. Uses Oil Red O is one of the dyes used for Sudan staining. Similar dyes include Sudan III, Sudan IV, and Sudan Black B. The staining has to be performed on fresh samples, as alcohol fixation removes most lipids. Oil Red O largely replaced Sudan III and Sudan IV, as it provides much deeper red color and the stains are therefore much easier to see. In pyrotechnics, Oil Red O is used in some compositions of red colored smokes. Forensic When staining, Oil Red O can make fat more visible in various cuts in pathology. It is also used in a technique (the method is called as the dye: Oil Red O), discovered in 2004 by Alexandre Beaudoin, for staining latent fingerprint A finge ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Periodic Acid-Schiff
Periodicity or periodic may refer to: Mathematics * Bott periodicity theorem, addresses Bott periodicity: a modulo-8 recurrence relation in the homotopy groups of classical groups * Periodic function, a function whose output contains values that repeat periodically * Periodic mapping Physical sciences * Periodic table of chemical elements * Periodic trends, relative characteristics of chemical elements observed * Redshift periodicity, astronomical term for redshift quantization Other uses * Fokker periodicity blocks, which mathematically relate musical intervals * Periodic acid, a compound of iodine * Principle of periodicity, a concept in generally accepted accounting principles * Quasiperiodicity, property of a system that displays irregular periodicity See also * Aperiodic (other) * Cycle (other) * Frequency (other) * Period (other) * Periodical * Seasonality In time series data, seasonality is the presence of variations that occur a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Eosin
Eosin is the name of several fluorescent acidic compounds which bind to and form salts with basic, or eosinophilic, compounds like proteins containing amino acid residues such as arginine and lysine, and stains them dark red or pink as a result of the actions of bromine on eosin. In addition to staining proteins in the cytoplasm, it can be used to stain collagen and muscle fibers for examination under the microscope. Structures, that stain readily with eosin, are termed eosinophilic. In the field of histology, Eosin Y is the form of eosin used most often as a histologic stain. Etymology Eosin was named by its inventor Heinrich Caro after the nickname (Eos) of a childhood friend, Anna Peters. Variants There are actually two very closely related compounds commonly referred to as eosin. Most often used is in histology is Eosin Y (also known as eosin Y ws, eosin yellowish, Acid Red 87, C.I. 45380, bromoeosine, bromofluoresceic acid, D&C Red No. 22); it has a very slightly yellowi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Hematoxylin
Haematoxylin or hematoxylin (), also called natural black 1 or C.I. 75290, is a compound extracted from heartwood of the logwood tree (''Haematoxylum campechianum'') with a chemical formula of . This naturally derived dye has been used as a histologic stain, ink and as a dye in the textile and leather industry. As a dye, haematoxylin has been called Palo de Campeche, logwood extract, bluewood and blackwood. In histology, haematoxylin staining is commonly followed (counterstained), with eosin, when paired, this staining procedure is known as H&E staining, and is one of the most commonly used combinations in histology. In addition to its use in the H&E stain, haematoxylin is also a component of the Papanicolaou stain (or PAP stain) which is widely used in the study of cytology specimens. Although the stain is commonly called ''haematoxylin'', the active colourant is the oxidized form haematein, which forms strongly coloured complexes with certain metal ions (commonly Fe(III) and ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
H&E Stain
Hematoxylin and eosin stain ( or haematoxylin and eosin stain or hematoxylin-eosin stain; often abbreviated as H&E stain or HE stain) is one of the principal tissue stains used in histology. It is the most widely used stain in medical diagnosis and is often the gold standard. For example, when a pathologist looks at a biopsy of a suspected cancer, the histological section is likely to be stained with H&E. H&E is the combination of two histological stains: hematoxylin and eosin. The hematoxylin stains cell nuclei a purplish blue, and eosin stains the extracellular matrix and cytoplasm pink, with other structures taking on different shades, hues, and combinations of these colors. Hence a pathologist can easily differentiate between the nuclear and cytoplasmic parts of a cell, and additionally, the overall patterns of coloration from the stain show the general layout and distribution of cells and provides a general overview of a tissue sample's structure. Thus, pattern recogniti ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Lithium Carbonate
Lithium carbonate is an inorganic compound, the lithium salt (chemistry), salt of carbonate with the chemical formula, formula . This white Salt (chemistry), salt is widely used in the processing of metal oxides. It is listed on the World Health Organization's List of Essential Medicines because it can be Lithium (medication), used as a treatment for mood disorders such as bipolar disorder. Uses Lithium carbonate is an important Industrial sector, industrial chemical. Its main use is as a precursor for compounds used in lithium-ion batteries. Glasses derived from lithium carbonate are useful in ovenware. Lithium carbonate is a common ingredient in both low-fire and high-fire ceramic glaze. It forms low-melting Flux (metallurgy), fluxes with silica and other materials. Its alkaline properties are conducive to changing the state of metal oxide colorants in Ceramic glaze, glaze, particularly red iron oxide (). Cement sets more rapidly when prepared with lithium carbonate, and is usef ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
