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List Of Single Cell Omics Methods
A list of more than 100 different single cell sequencing Single-cell sequencing examines the sequence information from individual cells with optimized next-generation sequencing technologies, providing a higher resolution of cellular differences and a better understanding of the function of an individual ... (omics) methods have been published. The large majority of methods are paired with short-read sequencing technologies, although some of them are compatible with long read sequencing. List References {{Reflist DNA sequencing ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Single Cell Sequencing
Single-cell sequencing examines the sequence information from individual cells with optimized next-generation sequencing technologies, providing a higher resolution of cellular differences and a better understanding of the function of an individual cell in the context of its microenvironment. For example, in cancer, sequencing the DNA of individual cells can give information about mutations carried by small populations of cells. In development, sequencing the RNAs expressed by individual cells can give insight into the existence and behavior of different cell types. In microbial systems, a population of the same species can appear genetically clonal. Still, single-cell sequencing of RNA or epigenetic modifications can reveal cell-to-cell variability that may help populations rapidly adapt to survive in changing environments. Background A typical human cell consists of about 2 x 3.3 billion base pairs of DNA and 600 million mRNA bases. Usually, a mix of millions of cells is used in ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
CyTOF
Cytometry by time of flight, or CyTOF, is an application of mass cytometry used to quantify labeled targets on the surface and interior of single cells. CyTOF allows the quantification of multiple cellular components simultaneously using an ICP-MS detector. CyTOF takes advantage of immunolabeling to quantify proteins, carbohydrates or lipids in a cell. Targets are selected to answer a specific research question and are labeled with lanthanide metal tagged antibodies. Labeled cells are nebulized and mixed with heated argon gas to dry the cell containing particles. The sample-gas mixture is focused and ignited with an argon plasma torch. This breaks the cells into their individual atoms and creates an ion cloud. Abundant low weight ions generated from environmental air and biological molecules are removed using a quadrupole mass analyzer. The remaining heavy ions from the antibody tags are quantified by Time-of-flight mass spectrometry. Ion abundances correlate with the amount of ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
G&T-Seq
G&T-seq (short for single cell genome and transcriptome sequencing) is a novel form of single cell sequencing technique allowing one to simultaneously obtain both transcriptomic and genomic data from single cells, allowing for direct comparison of gene expression data to its corresponding genomic data in the same cell... Background The advent of single-cell sequencing has provided researchers with the tools to resolve genotypically and phenotypically distinct cells within a mixed population. In cases where such heterogeneity is relevant, such as in tumours, this technique enables the study of clonal relationships and tumour evolution. As well, rare cell types and samples otherwise containing low cell numbers, such as in the case of circulating tumour cells, can also be studied in greater detail. However, previous methods of library preparation typically involve the capture of either mRNA or genomic DNA (gDNA), but not both. By simultaneously capturing and sequencing both D ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
10x Genomics
10x Genomics, Inc. is an American biotechnology company that designs and manufactures gene sequencing technology used in scientific research. It was founded in 2012 by Serge Saxonov, Ben Hindson, and Kevin Ness. History 10x Genomics was founded in 2012 by Serge Saxonov, Ben Hindson and Kevin Ness to create advanced testing equipment for use in Cell biology, cellular biology. Prior to starting the company, Saxonov was the founding architect, and director of research and development at 23andMe. Ness left 10x Genomics in December 2016 and in 2018, Justin McAnear, Tesla, Inc., Tesla's former finance chief joined the company as CFO. In August 2018 the company announced its first acquisition, Epinomics, a biotechnology company focused on the development of new techniques for epigenetics research. Four months later, 10x Genomics acquired Spatial Transcriptomics, a biotechnology company working in the field of spatial genomics. In November 2018, 10x Genomics announced expansion plans i ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
NanoString Technologies
NanoString Technologies, Inc. (NASDAQ: NSTG) is a publicly-held biotech company offering discovery and translational research solutions. NanoString's products include the nCounter® Gene Expression System, the GeoMx® Digital Spatial Profiler, the CosMx™ Spatial Molecular Imager, and the AtoMx™ Spatial Informatics Platform. These four systems enable scientists to envision molecular interactions in three dimensions and see the multiomic expression of genes and proteins in the context of tissue structure. NanoString products are based on a novel digital molecular barcoding technology invented at the Institute for Systems Biology under the direction of Dr. Leroy Hood. History The original patent forming the basis for the nCounter Analysis System was invented and licensed by The Institute for Systems Biology. The business plan was written by Amber Ratcliffe and Aaron Coe and won seed funding in multiple business plan competitions. NanoString was spun out of The Institute for S ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluidigm
Standard BioTools Inc. (formerly Fluidigm) is an American company engaged in the design, manufacture and sale of biological research equipment based on integrated fluidic circuit technology. The company's founders leveraged their semiconductor experience to create integrated circuits that carried fluids rather than electrons. In 2009, the company was described as "the world's leading manufacturer of microfluidic devices." Among the applications to which the company's products are put to use are protein crystallization, genotyping, DNA analysis and PCR. Business model Its products leverage the capabilities of multilayer soft lithography to create microfluidic devices: specifically, technology developed by one of the co-founders and branded "Integrated Fluidic Circuitry". It is a public company traded on the NASDAQ stock exchange, previously under the ticker symbol FLDM, and then under LAB. Fluidigm has a number of academic partners whose engagement is aimed to provide a wow ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
CITE-Seq
CITE-Seq (Cellular Indexing of Transcriptomes and Epitopes by Sequencing) is a method for performing RNA sequencing along with gaining quantitative and qualitative information on surface proteins with available antibodies on a single cell level. So far, the method has been demonstrated to work with only a few proteins per cell. As such, it provides an additional layer of information for the same cell by combining both proteomics and transcriptomics data. For phenotyping, this method has been shown to be as accurate as flow cytometry (a gold standard) by the groups that developed it. It is currently one of the main methods, along with REAP-Seq, to evaluate both gene expression and protein levels simultaneously in different species. The method was established by the New York Genome Center in collaboration with thSatija lab, while a similar approach was earlier shown bAbVitro Inc. Applications Concurrent measurement of both protein and transcript levels opens up opportunities to ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
SnRNA-seq
snRNA-seq, also known as single nucleus RNA sequencing, single nuclei RNA sequencing or sNuc-seq, is an RNA sequencing method for profiling gene expression in cells which are difficult to isolate, such as those from tissues that are archived or which are hard to be dissociated. It is an alternative to single cell RNA seq (scRNA-seq), as it analyzes nuclei instead of intact cells. snRNA-seq minimizes the occurrence of spurious gene expression, as the localization of fully mature ribosomes to the cytoplasm means that any mRNAs of transcription factor In molecular biology, a transcription factor (TF) (or sequence-specific DNA-binding factor) is a protein that controls the rate of transcription of genetic information from DNA to messenger RNA, by binding to a specific DNA sequence. The f ...s that are expressed after the dissociation process cannot be translated, and thus their downstream targets cannot be transcribed. Additionally, snRNA-seq technology enables the discov ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
