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Leishman Stain
Leishman stain, also known as Leishman's stain, is used in microscopy for staining blood smears. It is generally used to differentiate between and identify white blood cells, malaria parasites, and trypanosomas. It is based on a methanolic mixture of "polychromed" methylene blue (i.e. demethylated into various azures) and eosin. The methanolic stock solution is stable and also serves the purpose of directly fixing the smear eliminating a prefixing step. If a working solution is made by dilution with an aqueous buffer, the resulting mixture is very unstable and cannot be used for long. Leishman stain is named after its inventor, the Scottish pathologist William Boog Leishman. It is a version of the Romanowsky stain, and is thus similar to and partially replaceable by Giemsa stain, Jenner's stain, and Wright's stain. Preparation and storage Many companies sell Leishman Stain in the form of a dry powder, which is later reconstituted with methanol. Heat and bright light oxidize the ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Plasmodium Vivax Malaria
''Plasmodium'' is a genus of unicellular eukaryotes that are obligate parasites of vertebrates and insects. The life cycles of ''Plasmodium'' species involve development in a Hematophagy, blood-feeding insect host (biology), host which then injects parasites into a vertebrate host during a blood meal. Parasites grow within a vertebrate body tissue (often the liver) before entering the bloodstream to infect red blood cells. The ensuing destruction of host red blood cells can result in malaria. During this infection, some parasites are picked up by a blood-feeding insect (Mosquito, mosquitoes in majority cases), continuing the life cycle. ''Plasmodium'' is a member of the phylum Apicomplexa, a large group of parasitic eukaryotes. Within Apicomplexa, ''Plasmodium'' is in the order Haemosporida and family Plasmodiidae. Over 200 species of ''Plasmodium'' have been described, many of which have been subdivided into 14 subgenera based on parasite morphology and host range. Evolutionary ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Giemsa Stain
Giemsa stain (), named after German chemist and bacteriologist Gustav Giemsa, is a nucleic acid stain used in cytogenetics and for the histopathological diagnosis of malaria and other parasites. Uses It is specific for the phosphate groups of DNA and attaches itself to regions of DNA where there are high amounts of adenine-thymine bonding. Giemsa stain is used in Giemsa banding, commonly called G-banding, to stain chromosomes and often used to create a Karyotype, karyogram (chromosome map). It can identify chromosomal aberrations such as chromosomal translocation, translocations and chromosomal inversion, rearrangements. It stains the trophozoite ''Trichomonas vaginalis'', which presents with greenish discharge and motile cells on wet prep. Giemsa stain is also a Differential staining, differential stain, such as when it is combined with Wright's stain, Wright stain to form Wright-Giemsa stain. It can be used to study the adherence of pathogenic bacteria to human cells. It dif ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
H&E Stain
Hematoxylin and eosin stain ( or haematoxylin and eosin stain or hematoxylin-eosin stain; often abbreviated as H&E stain or HE stain) is one of the principal tissue stains used in histology. It is the most widely used stain in medical diagnosis and is often the gold standard. For example, when a pathologist looks at a biopsy of a suspected cancer, the histological section is likely to be stained with H&E. H&E is the combination of two histological stains: hematoxylin and eosin. The hematoxylin stains cell nuclei a purplish blue, and eosin stains the extracellular matrix and cytoplasm pink, with other structures taking on different shades, hues, and combinations of these colors. Hence a pathologist can easily differentiate between the nuclear and cytoplasmic parts of a cell, and additionally, the overall patterns of coloration from the stain show the general layout and distribution of cells and provides a general overview of a tissue sample's structure. Thus, pattern recogniti ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gustav Giemsa
Gustav Giemsa (; November 20, 1867 – June 10, 1948) was a German chemist and bacteriologist who was a native of Medar-Blechhammer (now part of the city Kędzierzyn-Koźle). He is remembered for creating a dye solution commonly known as "Giemsa stain". This dye is used for the histopathological diagnosis of malaria and parasites such as ''Plasmodium'', ''Trypanosoma'', and ''Chlamydia''. Giemsa studied pharmacy and mineralogy at the University of Leipzig, and chemistry and bacteriology at the University of Berlin. Between 1895 and 1898 he worked as a pharmacist in German East Africa. He was an early assistant to Bernhard Nocht at the '' Institut für Tropenmedizin'' in Hamburg, where in 1900 he became head of the Department of Chemistry. In 1904 Giemsa published an essay on the staining procedure for flagellates, blood cells, and bacteria. Giemsa improved the Romanowsky stain (Eosin Y and Methylene Blue) by stabilizing this dye solution with glycerol. This allowed for reproduci ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bernhard Nocht
Bernhard is both a given name and a surname. Notable people with the name include: Given name *Bernhard of Saxe-Weimar (1604–1639), Duke of Saxe-Weimar *Bernhard, Prince of Saxe-Meiningen (1901–1984), head of the House of Saxe-Meiningen 1946–1984 *Bernhard, Count of Bylandt (1905–1998), German nobleman, artist, and author *Prince Bernhard of Lippe-Biesterfeld (1911–2004), Prince Consort of Queen Juliana of the Netherlands *Bernhard, Hereditary Prince of Baden (born 1970), German prince *Bernhard Frank (1913–2011), German SS Commander *Bernhard Garside (born 1962), British diplomat *Bernhard Goetzke (1884–1964), German actor *Bernhard Grill (born 1961), one of the developers of MP3 technology *Bernhard Heiliger (1915–1995), German sculptor *Bernhard Langer (born 1957), German golfer *Bernhard Maier (born 1963), German celticist * Bernhard Raimann (born 1997), Austrian American football player *Bernhard Riemann (1826–1866), German mathematician *Bernhard Siebken ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Ernst Malachowski
Ernst is both a surname and a given name, the German, Dutch, and Scandinavian form of Ernest. Notable people with the name include: Surname * Adolf Ernst (1832–1899) German botanist known by the author abbreviation "Ernst" * Anton Ernst (1975-) South African Film Producer * Alice Henson Ernst (1880-1980), American writer and historian * Britta Ernst (born 1961), German politician * Cornelia Ernst, German politician * Edzard Ernst, German-British Professor of Complementary Medicine * Emil Ernst, astronomer * Ernie Ernst (1924/25–2013), former District Judge in Walker County, Texas * Eugen Ernst (1864–1954), German politician * Fabian Ernst, German soccer player * Gustav Ernst, Austrian writer * Heinrich Wilhelm Ernst, Moravian violinist and composer * Jim Ernst, Canadian politician * Jimmy Ernst, American painter, son of Max Ernst * Joni Ernst, U.S. Senator from Iowa * K.S. Ernst, American visual poet * Karl Friedrich Paul Ernst, German writer (1866–1933) * Ken Ernst, U.S. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Karl Reuter
Karl may refer to: People * Karl (given name), including a list of people and characters with the name * Karl der Große, commonly known in English as Charlemagne * Karl Marx, German philosopher and political writer * Karl of Austria, last Austrian Emperor * Karl (footballer) (born 1993), Karl Cachoeira Della Vedova Júnior, Brazilian footballer In myth * Karl (mythology), in Norse mythology, a son of Rig and considered the progenitor of peasants (churl) * ''Karl'', giant in Icelandic myth, associated with Drangey island Vehicles * Opel Karl, a car * ST ''Karl'', Swedish tugboat requisitioned during the Second World War as ST ''Empire Henchman'' Other uses * Karl, Germany, municipality in Rhineland-Palatinate, Germany * ''Karl-Gerät'', AKA Mörser Karl, 600mm German mortar used in the Second World War * KARL project, an open source knowledge management system * Korean Amateur Radio League, a national non-profit organization for amateur radio enthusiasts in South Korea * KARL, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
John Vivian Dacie
Sir John Vivian Dacie, FRS (20 July 1912 Putney, London – 12 February 2005) was a British haematologist. Education He was born in Putney, London and educated at King's College School, Wimbledon, after which he studied medicine at King's College Hospital Medical School, qualifying in 1936. Career He had house jobs at King's College Hospital, the Royal Postgraduate Medical School, London University, Hammersmith and a research post at Manchester Royal Infirmary. During World War II (1943–1946) he served in the Royal Army Medical Corps, ending up a lieutenant colonel. After the war he was a Senior Lecturer and then in 1956 Professor at the Royal Postgraduate Medical School. He founded the Leukaemia Research Fund, Great Ormond Street, London (1960). His main achievements concerned the Hemolytic anemias, a field in which he was a world leader. He discovered and named Christmas disease, more commonly referred to as haemophilia B, a deficiency of coagulation Factor IX. Sir Dac ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Methylene Violet
Methylene may refer to: * Methylene group or methylene bridge (CH2< or equivalently -CH2-), a part of a molecule connected to the rest of the molecule by two single bonds. * An older name for methylidene (=CH2), a part of a molecule connected to another atom by a double bond. * Methylene (compound) (CH2), an organic compound. See also * Bichloride of methylene (30% methanol and 70% chloroform), a variant of the old anesthetic A.C.E. mixture * Methyl group * Methylenedioxy * Mytilene, a city in Greece * Methanol Methanol (also called methyl alcohol and wood spirit, amongst other names) is an organic chemical and the simplest aliphatic alcohol, with the formula C H3 O H (a methyl group linked to a hydroxyl group, often abbreviated as MeOH). It is a ... {{disambiguation de:Methylengruppe sv:Metylengrupp ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Oxidize
Redox (reduction–oxidation, , ) is a type of chemical reaction in which the oxidation states of substrate change. Oxidation is the loss of electrons or an increase in the oxidation state, while reduction is the gain of electrons or a decrease in the oxidation state. There are two classes of redox reactions: * ''Electron-transfer'' – Only one (usually) electron flows from the reducing agent to the oxidant. This type of redox reaction is often discussed in terms of redox couples and electrode potentials. * ''Atom transfer'' – An atom transfers from one substrate to another. For example, in the rusting of iron, the oxidation state of iron atoms increases as the iron converts to an oxide, and simultaneously the oxidation state of oxygen decreases as it accepts electrons released by the iron. Although oxidation reactions are commonly associated with the formation of oxides, other chemical species can serve the same function. In hydrogenation, C=C (and other) bonds ar ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Wright's Stain
Wright's stain is a hematologic stain that facilitates the differentiation of blood cell types. It is classically a mixture of eosin (red) and methylene blue dyes. It is used primarily to stain peripheral blood smears, urine samples, and bone marrow aspirates, which are examined under a light microscope. In cytogenetics, it is used to stain chromosomes to facilitate diagnosis of syndromes and diseases. It is named for James Homer Wright, who devised the stain, a modification of the Romanowsky stain, in 1902. Because it distinguishes easily between blood cells, it became widely used for performing differential white blood cell counts, which are routinely ordered when conditions such as infection or leukemia are suspected. The related stains are known as the buffered Wright stain, the Wright-Giemsa stain (a combination of Wright and Giemsa stains), and the buffered Wright-Giemsa stain, and specific instructions depend on the solutions being used, which may include eosin Y, azure B, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Jenner's Stain
Jenner's stain (methylene blue eosinate) is used in microscopy for staining blood smear A blood smear, peripheral blood smear or blood film is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically. Blood smears are examined in the ...s. The stain is dark blue and results in very observable clearly stained nuclei. References Staining Romanowsky stains {{Med-diagnostic-stub ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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