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Heller's Test
Heller's test is a chemical test that shows that strong acids cause the denaturation of precipitated proteins. Concentrated nitric acid is added to a protein solution from the side of the test tube to form two layers. A white ring appears between the two layers if the test is positive. Heller's test is commonly used to test for the presence of proteins in urine Urine is a liquid by-product of metabolism in humans and many other animals. In placental mammals, urine flows from the Kidney (vertebrates), kidneys through the ureters to the urinary bladder and exits the urethra through the penile meatus (mal .... This test was discovered by the Austrian Chemist, Johann Florian Heller (1813-1871). References Chemical tests {{analytical-chemistry-stub ...
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Chemical Test
In chemistry, a chemical test is a qualitative property, qualitative or Quantitative property, quantitative procedure designed to identify, quantify, or characterise a chemical compound or substituent, chemical group. Purposes Chemical testing might have a variety of purposes, such as to: * Determine if, or verify that, the requirements of a specification, regulation, or contract are met * Decide if a new product development program is on track: Demonstrate proof of concept * Demonstrate the utility of a proposed patent * Determine the interactions of a sample with other known substances * Determine the composition of a sample * Provide Technical standard, standard data for other scientific, medical, and Quality assurance functions * Validate suitability for end-use * Provide a basis for Technical communication * Provide a technical means of comparison of several options * Provide evidence in legal proceedings Biochemical tests * Clinistrips quantitatively test for sugar in uri ...
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Strong Acid
Acid strength is the tendency of an acid, symbolised by the chemical formula , to dissociate into a hydron (chemistry), proton, , and an anion, . The Dissociation (chemistry), dissociation or ionization of a strong acid in solution is effectively complete, except in its most concentrated solutions. : Examples of strong acids are hydrochloric acid (), perchloric acid (), nitric acid () and sulfuric acid (). A weak acid is only partially dissociated, or is partly ionized in water with both the undissociated acid and its dissociation products being present, in solution, in Equilibrium chemistry, equilibrium with each other. : Acetic acid () is an example of a weak acid. The strength of a weak acid is quantified by its acid dissociation constant, K_a value. The strength of a weak organic chemistry, organic acid may depend on substituent effects. The strength of an inorganic chemistry, inorganic acid is dependent on the oxidation state for the atom to which the proton may be atta ...
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Denaturation (biochemistry)
In biochemistry, denaturation is a process in which proteins or nucleic acids lose folded structure present in their native state due to various factors, including application of some external stress or compound, such as a strong acid or base, a concentrated inorganic salt, an organic solvent (e.g., alcohol or chloroform), agitation, radiation, or heat. If proteins in a living cell are denatured, this results in disruption of cell activity and possibly cell death. Protein denaturation is also a consequence of cell death. Denatured proteins can exhibit a wide range of characteristics, from conformational change and loss of solubility or dissociation of cofactors to aggregation due to the exposure of hydrophobic groups. The loss of solubility as a result of denaturation is called ''coagulation''. Denatured proteins, e.g., metalloenzymes, lose their 3D structure or metal cofactor, and therefore, cannot function. Proper protein folding is key to whether a globular or memb ...
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Nitric Acid
Nitric acid is an inorganic compound with the formula . It is a highly corrosive mineral acid. The compound is colorless, but samples tend to acquire a yellow cast over time due to decomposition into nitrogen oxide, oxides of nitrogen. Most commercially available nitric acid has a concentration of 68% in water. When the solution contains more than 86% , it is referred to as ''fuming nitric acid''. Depending on the amount of nitrogen dioxide present, fuming nitric acid is further characterized as red fuming nitric acid at concentrations above 86%, or white fuming nitric acid at concentrations above 95%. Nitric acid is the primary reagent used for nitration – the addition of a nitro group, typically to an organic molecule. While some resulting nitro compounds are shock- and thermally-sensitive explosives, a few are stable enough to be used in munitions and demolition, while others are still more stable and used as synthetic dyes and medicines (e.g. metronidazole). Nitric acid is ...
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Urine
Urine is a liquid by-product of metabolism in humans and many other animals. In placental mammals, urine flows from the Kidney (vertebrates), kidneys through the ureters to the urinary bladder and exits the urethra through the penile meatus (males) or urethral meatus of the vulva (females) during urination. In other vertebrates, urine is excreted through the cloaca. Urine contains water-soluble by-products of Cell (biology), cellular metabolism that are rich in nitrogen and must be clearance (medicine), cleared from the Circulatory system, bloodstream, such as urea, uric acid and creatinine. A urinalysis can detect nitrogenous wastes of the mammalian body. Urine plays an important role in the earth's nitrogen cycle. In balanced ecosystems, urine fertilizes the soil and thus helps plants to grow. Therefore, Reuse of excreta, urine can be used as a fertilizer. Some animals use it to territory (animal)#Scent marking, mark their territories. Historically, aged or fermented urine (kn ...
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Johann Florian Heller
Johann Florian Heller (4 May 1813 – 21 November 1871) was an Austrian chemist who was one of the founders of clinical chemistry. Heller was born in Vienna, Austria. He studied chemistry in Prague and later with Liebig and Wöhler at Giessen. Louis Rosenfeld (1999), ''Four centuries of clinical chemistry''. CRC PressOnline versionaccessed on 2009-07-30. Giuliano dall'Olio (2008), ''Nuovo componente dei calcoli vescicali — L’ “urostealite ” di Heller''. RIMeL - IJLaM, volume 4, issue 1, Società Italiana Medicina di Laboratorio. During those studies he characterized rhodizonic acid and its potassium salt (1837). Johann Florian Heller (1837), ''Die Rhodizonsäure, eine aus den Produkten der Kaliumbereitung gewonnene neue Säure, und ihre chemischen Verhältnisse'', Justus Liebigs Annalen der Pharmacie, volume 24, issue 1, pp. 1–16Online versionaccessed on 2009-07-08. In 1844 Heller established a laboratory of pathological chemistry in Vienna's General Hospital ...
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