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Fluorescence Imaging
Fluorescence imaging is a type of non-invasive imaging technique that can help visualize biological processes taking place in a living organism. Images can be produced from a variety of methods including: microscopy, imaging probes, and spectroscopy. Fluorescence itself, is a form of luminescence that results from matter emitting light of a certain wavelength after absorbing electromagnetic radiation. Molecules that re-emit light upon absorption of light are called fluorophores. Fluorescence imaging photographs fluorescent dyes and fluorescent proteins to mark molecular mechanisms and structures. It allows one to experimentally observe the dynamics of gene expression, protein expression, and molecular interactions in a living cell. It essentially serves as a precise, quantitative tool regarding biochemical applications. A common misconception, fluorescence differs from bioluminescence by how the proteins from each process produce light. Bioluminescence is a chemical process that ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Multicolor Fluorescence Image Of Living HeLa Cells
Multicolor is a Subtractive color, subtractive two-color Color motion picture film, motion picture process. Multicolor, introduced to the motion picture industry in 1929, was based on the earlier Prizma, Prizma Color process, and was the forerunner of Cinecolor. For a Multicolor film, a scene is shot with a normal camera capable of bipacking film. Two black-and-white 35mm movie film, 35mm film negatives are threaded bipack in the camera. One records the color red (via a dyed panchromatic film), and the other, blue (orthochromatic). In printing, Duplitized film, duplitized stock is exposed and processed with one record on each side. In a tank of Film tinting, toning solution, the film is floated upon the top of the solution with the appropriate chemical. The cyan record is toned a complementary red with a copper ferrocyanide solution, and the red being toned blue/cyan with ferric ferrocyanide solution. Multicolor enjoyed brief success in early sound pictures. The following f ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Jablonski Diagram Of Fluorescence Only-en
Jabłoński (Polish pronunciation: ; feminine: Jabłońska; plural: Jabłońscy) is a Polish surname derived from the noun ''jabłoń'' (''apple tree''). It appears in various forms when transliterated from Cyrillic alphabets. People * Aleksander Jabłoński (1898–1980), Polish physicist * Benedict Jablonski (1917–2003), science fiction fan and booster * Carl Gustav Jablonsky (1756–1787), Berlin naturalist, entomologist and illustrator * Constance Jablonski (born 1990), French fashion model * Daniel Ernst Jablonski (1660–1741), German theologian and reformer * Dariusz Jabłoński (born 1961), Polish film director and producer * David Jablonski (born 1953), American professor of geophysical sciences * Edward Jablonski (1923–2004), American author * Edward Jabłoński (1919–1970), Polish football player * Grigoriy Yablonsky (born 1940), Russian chemist * Hanna Yablonska (1981–2011), Ukrainian playwright and poet * Henryk Jabłoński (1909–2003), Polish socialist ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorescence Microscope
A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances. "Fluorescence microscope" refers to any microscope that uses fluorescence to generate an image, whether it is a simple set up like an epifluorescence microscope or a more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescence image. Principle The specimen is illuminated with light of a specific wavelength (or wavelengths) which is absorbed by the fluorophores, causing them to emit light of longer wavelengths (i.e., of a different color than the absorbed light). The illumination light is separated from the much weaker emitted fluorescence through the use of a spectral emission filter. Typical components of a fluorescence microscope are a light source (xenon arc lamp or mercury-vapor lamp are ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorescence In The Life Sciences
Fluorescence is used in the life sciences generally as a non-destructive way of tracking or analysing biological molecules. Some proteins or small molecules in cells are naturally fluorescent, which is called intrinsic fluorescence or autofluorescence (such as NADH, tryptophan or endogenous chlorophyll, phycoerythrin or green fluorescent protein). Alternatively, specific or general proteins, nucleic acids, lipids or small molecules can be "labelled" with an extrinsic fluorophore, a fluorescent dye which can be a small molecule, protein or quantum dot. Several techniques exist to exploit additional properties of fluorophores, such as fluorescence resonance energy transfer, where the energy is passed non-radiatively to a particular neighbouring dye, allowing proximity or protein activation to be detected; another is the change in properties, such as intensity, of certain dyes depending on their environment allowing their use in structural studies. Fluorescence The principle b ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorescence From Fluorescent Proteins
Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore a lower photon energy, than the absorbed radiation. A perceptible example of fluorescence occurs when the absorbed radiation is in the ultraviolet region of the electromagnetic spectrum (invisible to the human eye), while the emitted light is in the visible region; this gives the fluorescent substance a distinct color that can only be seen when the substance has been exposed to UV light. Fluorescent materials cease to glow nearly immediately when the radiation source stops, unlike phosphorescent materials, which continue to emit light for some time after. Fluorescence has many practical applications, including mineralogy, gemology, medicine, chemical sensors (fluorescence spectroscopy), fluorescent labelling, dyes, biological detectors, cosmic-ray detection, vacuum ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Agarose Gel With UV Illumination - Ethidium Bromide Stained DNA Glows Orange (close-up)
Agarose is a heteropolysaccharide, generally extracted from certain red seaweed. It is a linear polymer made up of the repeating unit of agarobiose, which is a disaccharide made up of D-galactose and 3,6-anhydro-L-galactopyranose. Agarose is one of the two principal components of agar, and is purified from agar by removing agar's other component, agaropectin. Agarose is frequently used in molecular biology for the separation of large molecules, especially DNA, by electrophoresis. Slabs of agarose gels (usually 0.7 - 2%) for electrophoresis are readily prepared by pouring the warm, liquid solution into a mold. A wide range of different agaroses of varying molecular weights and properties are commercially available for this purpose. Agarose may also be formed into beads and used in a number of chromatographic methods for protein purification. Structure Agarose is a linear polymer with a molecular weight of about 120,000, consisting of alternating D-galactose and 3,6-anhydro- ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
GFP Mice 01
GFP may refer to: Organisations * Gaelic Football Provence, a French Gaelic Athletic Association club * Geheime Feldpolizei, the German secret military police during the Second World War * French Group for the Study of Polymers and their Application, also called French Polymer Group, a French society for the promotion of polymer science Politics * GFP Ramdir Sena, a militant Hindu nationalist group in Nepal * Goa Forward Party, a political party in Goa, India * Great Fatherland Party, a Russian political party Science and technology * Generic Framing Procedure, a multiplexing technique * Greatest fixed point, in mathematics * Green fluorescent protein * Ground-fault protection, an electrical safety device Other uses * Generalized first-price auction * Government-Furnished Property, a term of art for property furnished by the US Federal government to fulfill contract obligations under Federal Acquisition Regulation The Federal Acquisition Regulation (FAR) is the principal set of r ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bioluminescence
Bioluminescence is the production and emission of light by living organisms. It is a form of chemiluminescence. Bioluminescence occurs widely in marine vertebrates and invertebrates, as well as in some fungi, microorganisms including some bioluminescent bacteria, and terrestrial arthropods such as fireflies. In some animals, the light is bacteriogenic, produced by symbiotic bacteria such as those from the genus ''Vibrio''; in others, it is autogenic, produced by the animals themselves. In a general sense, the principal chemical reaction in bioluminescence involves a light-emitting molecule and an enzyme, generally called luciferin and luciferase, respectively. Because these are generic names, luciferins and luciferases are often distinguished by the species or group, e.g. firefly luciferin. In all characterized cases, the enzyme catalyzes the oxidation of the luciferin. In some species, the luciferase requires other cofactors, such as calcium or magnesium ions, and somet ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Biological Processes
Biological processes are those processes that are vital for an organism to live, and that shape its capacities for interacting with its environment. Biological processes are made of many chemical reactions or other events that are involved in the persistence and transformation of life forms. Metabolism and homeostasis are examples. Biological processes within an organism can also work as bioindicators. Scientists are able to look at an individual's biological processes to monitor the effects of environmental changes. Regulation of biological processes occurs when any process is modulated in its frequency, rate or extent. Biological processes are regulated by many means; examples include the control of gene expression, protein modification or interaction with a protein or substrate molecule. * Homeostasis: regulation of the internal environment to maintain a constant state; for example, sweating to reduce temperature * Organization: being structurally composed of one or more cell ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gene Expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product that enables it to produce end products, protein or non-coding RNA, and ultimately affect a phenotype, as the final effect. These products are often proteins, but in non-protein-coding genes such as transfer RNA (tRNA) and small nuclear RNA (snRNA), the product is a functional non-coding RNA. Gene expression is summarized in the central dogma of molecular biology first formulated by Francis Crick in 1958, further developed in his 1970 article, and expanded by the subsequent discoveries of reverse transcription and RNA replication. The process of gene expression is used by all known life—eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses—to generate the macromolecular machinery for life. In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, '' ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorophores
A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with several π bonds. Fluorophores are sometimes used alone, as a tracer in fluids, as a dye for staining of certain structures, as a substrate of enzymes, or as a probe or indicator (when its fluorescence is affected by environmental aspects such as polarity or ions). More generally they are covalently bonded to a macromolecule, serving as a marker (or dye, or tag, or reporter) for affine or bioactive reagents (antibodies, peptides, nucleic acids). Fluorophores are notably used to stain tissues, cells, or materials in a variety of analytical methods, i.e., fluorescent imaging and spectroscopy. Fluorescein, via its amine-reactive isothiocyanate derivative fluorescein isothiocyanate (FITC), has been one of the most popular fluorophores. From an ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
