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Flow Cytometry Standard
Flow Cytometry Standard (FCS) is a data file standard for the reading and writing of data from flow cytometry experiments. The FCS specification has traditionally been developed and maintained by the International Society for Advancement of Cytometry (ISAC). FCS used to be the only widely adopted file format in flow cytometry. Recently, additional standard file formats have been developed by ISAC. File Format The FCS file format describes a file that is a combination of textual data followed by binary data. The order of the file layout is as follows: # HEADER segment # TEXT segment # DATA segment # Optional ANALYSIS segment # CRC value # Optional OTHER segments The HEADER segment is an ASCII text string that begins by identifying the version of the FCS standard used, followed by three pairs of byte offsets that designate the positions of the TEXT, DATA, and ANALYSIS segments. An example header segment is given below FCS3.0 58 4380 4381 5586 0 0 Because ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Flow Cytometry
Flow cytometry (FC) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. Cells are often labeled with fluorescent markers so light is absorbed and then emitted in a band of wavelengths. Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer. Flow cytometry is routinely used in basic research, clinical practice, and clinical trials. Uses for flow cytometry include: * Cell counting * Cell sorting * Determining cell characteristics and function * Detecting microorganisms * Biomarker detection * Protein engineering detection * Diagnosis of health disorders such as blood cancers * Measuring ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
International Society For Advancement Of Cytometry
International is an adjective (also used as a noun) meaning "between nations". International may also refer to: Music Albums * ''International'' (Kevin Michael album), 2011 * ''International'' (New Order album), 2002 * ''International'' (The Three Degrees album), 1975 *''International'', 2018 album by L'Algérino Songs * The Internationale, the left-wing anthem * "International" (Chase & Status song), 2014 * "International", by Adventures in Stereo from ''Monomania'', 2000 * "International", by Brass Construction from ''Renegades'', 1984 * "International", by Thomas Leer from ''The Scale of Ten'', 1985 * "International", by Kevin Michael from ''International'' (Kevin Michael album), 2011 * "International", by McGuinness Flint from ''McGuinness Flint'', 1970 * "International", by Orchestral Manoeuvres in the Dark from '' Dazzle Ships'', 1983 * "International (Serious)", by Estelle from '' All of Me'', 2012 Politics * Political international, any transnational organization ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Array (data Type)
In computer science, array is a data type that represents a collection of ''elements'' (values or variables), each selected by one or more indices (identifying keys) that can be computed at run time during program execution. Such a collection is usually called an array variable or array value.Robert W. Sebesta (2001) ''Concepts of Programming Languages''. Addison-Wesley. 4th edition (1998), 5th edition (2001), By analogy with the mathematical concepts vector and matrix, array types with one and two indices are often called vector type and matrix type, respectively. More generally, a multidimensional array type can be called a tensor type, by anology with the physical concept, tensor. Language support for array types may include certain built-in array data types, some syntactic constructions (''array type constructors'') that the programmer may use to define such types and declare array variables, and special notation for indexing array elements. For example, in the Pascal p ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
File Format
A file format is a standard way that information is encoded for storage in a computer file. It specifies how bits are used to encode information in a digital storage medium. File formats may be either proprietary or free. Some file formats are designed for very particular types of data: PNG files, for example, store bitmapped images using lossless data compression. Other file formats, however, are designed for storage of several different types of data: the Ogg format can act as a container for different types of multimedia including any combination of audio and video, with or without text (such as subtitles), and metadata. A text file can contain any stream of characters, including possible control characters, and is encoded in one of various character encoding schemes. Some file formats, such as HTML, scalable vector graphics, and the source code of computer software are text files with defined syntaxes that allow them to be used for specific purposes. Specifications ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Binary File
A binary file is a computer file that is not a text file. The term "binary file" is often used as a term meaning "non-text file". Many binary file formats contain parts that can be interpreted as text; for example, some computer document files containing formatted text, such as older Microsoft Word document files, contain the text of the document but also contain formatting information in binary form. Structure Binary files are usually thought of as being a sequence of bytes, which means the binary digits (bits) are grouped in eights. Binary files typically contain bytes that are intended to be interpreted as something other than text characters. Compiled computer programs are typical examples; indeed, compiled applications are sometimes referred to, particularly by programmers, as binaries. But binary files can also mean that they contain images, sounds, compressed versions of other files, etc. – in short, any type of file content whatsoever. Some binary files contain ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Endianness
In computing, endianness, also known as byte sex, is the order or sequence of bytes of a word of digital data in computer memory. Endianness is primarily expressed as big-endian (BE) or little-endian (LE). A big-endian system stores the most significant byte of a word at the smallest memory address and the least significant byte at the largest. A little-endian system, in contrast, stores the least-significant byte at the smallest address. Bi-endianness is a feature supported by numerous computer architectures that feature switchable endianness in data fetches and stores or for instruction fetches. Other orderings are generically called middle-endian or mixed-endian. Endianness may also be used to describe the order in which the bits are transmitted over a communication channel, e.g., big-endian in a communications channel transmits the most significant bits first. Bit-endianness is seldom used in other contexts. Etymology Danny Cohen introduced the terms ''big-endian'' a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Biological Specimen
A biological specimen (also called a biospecimen) is a biological laboratory specimen held by a biorepository for research. Such a specimen would be taken by sampling so as to be representative of any other specimen taken from the source of the specimen. When biological specimens are stored, ideally they remain equivalent to freshly-collected specimens for the purposes of research. Human biological specimens are stored in a type of biorepository called a biobank, and the science of preserving biological specimens is most active in the field of biobanking. Quality control Setting broad standards for quality of biological specimens was initially an underdeveloped aspect of biobank growth. There is currently discussion on what standards should be in place and who should manage those standards. Since many organizations set their own standards and since biobanks are necessarily used by multiple organizations and typically are driven towards expansion, the harmonization of standard ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Flow Cytometry
Flow cytometry (FC) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. Cells are often labeled with fluorescent markers so light is absorbed and then emitted in a band of wavelengths. Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer. Flow cytometry is routinely used in basic research, clinical practice, and clinical trials. Uses for flow cytometry include: * Cell counting * Cell sorting * Determining cell characteristics and function * Detecting microorganisms * Biomarker detection * Protein engineering detection * Diagnosis of health disorders such as blood cancers * Measuring ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Flow Cytometry Bioinformatics
Flow cytometry bioinformatics is the application of bioinformatics to flow cytometry data, which involves storing, retrieving, organizing and analyzing flow cytometry data using extensive computational resources and tools. Flow cytometry bioinformatics requires extensive use of and contributes to the development of techniques from computational statistics and machine learning. Flow cytometry and related methods allow the quantification of multiple independent biomarkers on large numbers of single cells. The rapid growth in the multidimensionality and throughput of flow cytometry data, particularly in the 2000s, has led to the creation of a variety of computational analysis methods, data standards, and public databases for the sharing of results. Computational methods exist to assist in the preprocessing of flow cytometry data, identifying cell populations within it, matching those cell populations across samples, and performing diagnosis and discovery using the results of previous ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
