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Fecal Egg Count
Eggs per gram (eggs/g) is a laboratory test that determines the number of eggs per gram of feces in patients suspected of having a parasitological infection, such as schistosomiasis. Measuring the number of eggs per gram is the primary diagnostic method for schistosomiasis, as opposed to a blood test. Eggs per gram or another analyse like larvae per gram of faeces is one of the most important experiments that is done in parasitology labs. Methods to count the number of eggs per gram: * Willis method * McMaster method * Clayton-Lane method See also * Kato technique The Kato technique (also called the Kato–Katz technique) is a laboratory method for preparing human stool samples prior to searching for parasite eggs. Indications The Kato technique is now most commonly used for detecting schistosome eggs. I ... * Helminths References External links * Parasitology Clinical pathology {{Pathology-stub ...
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Egg (biology)
An egg is an organic vessel grown by an animal to carry a possibly fertilized egg cell (a zygote) and to incubate from it an embryo within the egg until the embryo has become an animal fetus that can survive on its own, at which point the animal hatches. Most arthropods such as insects, vertebrates (excluding live-bearing mammals), and mollusks lay eggs, although some, such as scorpions, do not. Reptile eggs, bird eggs, and monotreme eggs are laid out of water and are surrounded by a protective shell, either flexible or inflexible. Eggs laid on land or in nests are usually kept within a warm and favorable temperature range while the embryo grows. When the embryo is adequately developed it hatches, i.e., breaks out of the egg's shell. Some embryos have a temporary egg tooth they use to crack, pip, or break the eggshell or covering. The largest recorded egg is from a whale shark and was in size. Whale shark eggs typically hatch within the mother. At and up to , the o ...
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Gram
The gram (originally gramme; SI unit symbol g) is a Physical unit, unit of mass in the International System of Units (SI) equal to one one thousandth of a kilogram. Originally defined as of 1795 as "the absolute weight of a volume of pure water equal to Cube (algebra), the cube of the hundredth part of a metre [1 Cubic centimetre, cm3], and at Melting point of water, the temperature of Melting point, melting ice", the defining temperature (~0 °C) was later changed to 4 °C, the temperature of maximum density of water. However, by the late 19th century, there was an effort to make the Base unit (measurement), base unit the kilogram and the gram a derived unit. In 1960, the new International System of Units defined a ''gram'' as one one-thousandth of a kilogram (i.e., one gram is Scientific notation, 1×10−3 kg). The kilogram, 2019 redefinition of the SI base units, as of 2019, is defined by the International Bureau of Weights and Measures from the fixed numeric ...
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Feces
Feces ( or faeces), known colloquially and in slang as poo and poop, are the solid or semi-solid remains of food that was not digested in the small intestine, and has been broken down by bacteria in the large intestine. Feces contain a relatively small amount of metabolic waste products such as bacterially altered bilirubin, and dead epithelial cells from the lining of the gut. Feces are discharged through the anus or cloaca during defecation. Feces can be used as fertilizer or soil conditioner in agriculture. They can also be burned as fuel or dried and used for construction. Some medicinal uses have been found. In the case of human feces, fecal transplants or fecal bacteriotherapy are in use. Urine and feces together are called excreta. Skatole is the principal compound responsible for the unpleasant smell of feces. Characteristics The distinctive odor of feces is due to skatole, and thiols (sulfur-containing compounds), as well as amines and carboxylic acids. Skatole ...
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Parasitological
Parasitology is the study of parasites, their hosts, and the relationship between them. As a biological discipline, the scope of parasitology is not determined by the organism or environment in question but by their way of life. This means it forms a synthesis of other disciplines, and draws on techniques from fields such as cell biology, bioinformatics, biochemistry, molecular biology, immunology, genetics, evolution and ecology. Fields The study of these diverse organisms means that the subject is often broken up into simpler, more focused units, which use common techniques, even if they are not studying the same organisms or diseases. Much research in parasitology falls somewhere between two or more of these definitions. In general, the study of prokaryotes falls under the field of bacteriology rather than parasitology. Medical The parasitologist F.E.G. Cox noted that "Humans are hosts to nearly 300 species of parasitic worms and over 70 species of protozoa, some derived f ...
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Infection
An infection is the invasion of tissues by pathogens, their multiplication, and the reaction of host tissues to the infectious agent and the toxins they produce. An infectious disease, also known as a transmissible disease or communicable disease, is an illness resulting from an infection. Infections can be caused by a wide range of pathogens, most prominently bacteria and viruses. Hosts can fight infections using their immune system. Mammalian hosts react to infections with an innate response, often involving inflammation, followed by an adaptive response. Specific medications used to treat infections include antibiotics, antivirals, antifungals, antiprotozoals, and antihelminthics. Infectious diseases resulted in 9.2 million deaths in 2013 (about 17% of all deaths). The branch of medicine that focuses on infections is referred to as infectious disease. Types Infections are caused by infectious agents (pathogens) including: * Bacteria (e.g. ''Mycobacterium tuberculosis'', ...
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Schistosomiasis
Schistosomiasis, also known as snail fever, bilharzia, and Katayama fever, is a disease caused by parasitic flatworms called schistosomes. The urinary tract or the intestines may be infected. Symptoms include abdominal pain, diarrhea, bloody stool, or blood in the urine. Those who have been infected for a long time may experience liver damage, kidney failure, infertility, or bladder cancer. In children, it may cause poor growth and learning difficulty. The disease is spread by contact with fresh water contaminated with the parasites. These parasites are released from infected freshwater snails. The disease is especially common among children in developing countries, as they are more likely to play in contaminated water. Other high-risk groups include farmers, fishermen, and people using unclean water during daily living. It belongs to the group of helminth infections. Diagnosis is by finding eggs of the parasite in a person's urine or stool. It can also be confirmed by fi ...
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Blood Test
A blood test is a laboratory analysis performed on a blood sample that is usually extracted from a vein in the arm using a hypodermic needle, or via fingerprick. Multiple tests for specific blood components, such as a glucose test or a cholesterol test, are often grouped together into one test panel called a blood panel or blood work. Blood tests are often used in health care to determine physiological and biochemical states, such as disease, mineral content, pharmaceutical drug effectiveness, and organ function. Typical clinical blood panels include a basic metabolic panel or a complete blood count. Blood tests are also used in drug tests to detect drug abuse. Extraction A venipuncture is useful as it is a minimally invasive way to obtain cells and extracellular fluid ( plasma) from the body for analysis. Blood flows throughout the body, acting as a medium that provides oxygen and nutrients to tissues and carries waste products back to the excretory systems for disposal. Co ...
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Larvae
A larva (; plural larvae ) is a distinct juvenile form many animals undergo before metamorphosis into adults. Animals with indirect development such as insects, amphibians, or cnidarians typically have a larval phase of their life cycle. The larva's appearance is generally very different from the adult form (''e.g.'' caterpillars and butterflies) including different unique structures and organs that do not occur in the adult form. Their diet may also be considerably different. Larvae are frequently adapted to different environments than adults. For example, some larvae such as tadpoles live almost exclusively in aquatic environments, but can live outside water as adult frogs. By living in a distinct environment, larvae may be given shelter from predators and reduce competition for resources with the adult population. Animals in the larval stage will consume food to fuel their transition into the adult form. In some organisms like polychaetes and barnacles, adults are immobil ...
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Faeces
Feces ( or faeces), known colloquially and in slang as poo and poop, are the solid or semi-solid remains of food that was not digested in the small intestine, and has been broken down by bacteria in the large intestine. Feces contain a relatively small amount of metabolic waste products such as bacterially altered bilirubin, and dead epithelial cells from the lining of the gut. Feces are discharged through the anus or cloaca during defecation. Feces can be used as fertilizer or soil conditioner in agriculture. They can also be burned as fuel or dried and used for construction. Some medicinal uses have been found. In the case of human feces, fecal transplants or fecal bacteriotherapy are in use. Urine and feces together are called excreta. Skatole is the principal compound responsible for the unpleasant smell of feces. Characteristics The distinctive odor of feces is due to skatole, and thiols (sulfur-containing compounds), as well as amines and carboxylic acids. Skatole ...
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Kato Technique
The Kato technique (also called the Kato–Katz technique) is a laboratory method for preparing human stool samples prior to searching for parasite eggs. Indications The Kato technique is now most commonly used for detecting schistosome eggs. It has in the past been used for other helminth eggs as well. It cannot be used to identify hookworm eggs because they collapse within 30 to 60 minutes of preparation using this method. One study of 299 subjects infected with ''Schistosoma mansoni'' found that the method had poor reproducibility and is therefore no longer recommended for primary health care settings: the problem may be that eggs of ''S. mansoni ''tend to clump together which means that even slides prepared from the same specimen may contain widely different egg counts. The other main argument against the Kato technique is that it is messy and therefore exposes technicians to an unnecessarily high risk of infection. Method The published methods vary in detail, but genera ...
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Helminths
Parasitic worms, also known as helminths, are large macroparasites; adults can generally be seen with the naked eye. Many are intestinal worms that are soil-transmitted and infect the gastrointestinal tract. Other parasitic worms such as schistosomes reside in blood vessels. Some parasitic worms, including leeches and monogeneans, are ectoparasites thus, they are not classified as helminths, which are endoparasites. Parasitic worms live in and feed in living hosts. They receive nourishment and protection while disrupting their hosts' ability to absorb nutrients. This can cause weakness and disease in the host, and poses a global health and economic problem. Parasitic worms cannot reproduce entirely within their host's body; they have a life cycle that includes some stages that need to take place outside of the host. Helminths are able to survive in their mammalian hosts for many years due to their ability to manipulate the host's immune response by secreting immunomodulatory ...
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Parasitology
Parasitology is the study of parasites, their hosts, and the relationship between them. As a biological discipline, the scope of parasitology is not determined by the organism or environment in question but by their way of life. This means it forms a synthesis of other disciplines, and draws on techniques from fields such as cell biology, bioinformatics, biochemistry, molecular biology, immunology, genetics, evolution and ecology. Fields The study of these diverse organisms means that the subject is often broken up into simpler, more focused units, which use common techniques, even if they are not studying the same organisms or diseases. Much research in parasitology falls somewhere between two or more of these definitions. In general, the study of prokaryotes falls under the field of bacteriology rather than parasitology. Medical The parasitologist F.E.G. Cox noted that "Humans are hosts to nearly 300 species of parasitic worms and over 70 species of protozoa, some derived f ...
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