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DGCR8
The microprocessor complex subunit DGCR8 ''(DiGeorge syndrome critical region 8)'' is a protein that in humans is encoded by the gene. In other animals, particularly the common model organisms ''Drosophila melanogaster'' and ''Caenorhabditis elegans'', the protein is known as ''Pasha'' (partner of Drosha). It is a required component of the RNA interference pathway. Function The subunit DGCR8 is localized to the cell nucleus and is required for microRNA (miRNA) processing. It binds to the other subunit Drosha, an RNase III enzyme, to form the microprocessor complex that cleaves a primary transcript known as pri-miRNA to a characteristic stem-loop structure known as a pre-miRNA, which is then further processed to miRNA fragments by the enzyme Dicer. DGCR8 contains an RNA-binding domain and is thought to bind pri-miRNA to stabilize it for processing by Drosha. DGCR8 is also required for some types of DNA repair. Removal of UV-induced DNA Pyrimidine dimer, photoproducts, during ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Microprocessor Complex
The microprocessor complex is a protein complex involved in the early stages of processing microRNA (miRNA) and RNA interference (RNAi) in animal cells. The complex is minimally composed of the ribonuclease enzyme Drosha and the dimeric RNA-binding protein DGCR8 (also known as Pasha in non-human animals), and cleaves primary miRNA substrates to pre-miRNA in the cell nucleus. Microprocessor is also the smaller of the two multi-protein complexes that contain human Drosha. Composition The microprocessor complex consists minimally of two proteins: Drosha, a ribonuclease III enzyme; and DGCR8, a double-stranded RNA binding protein. (DGCR8 is the name used in mammalian genetics, abbreviated from "DiGeorge syndrome critical region 8"; the homologous protein in model organisms such as flies and worms is called ''Pasha'', for ''Pa''rtner of Dro''sha''.) The stoichiometry of the minimal complex was at one point experimentally difficult to determine, but it has been demonstrated to be a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
