Corynebacterium Amycolatum
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Corynebacterium Amycolatum
''Corynebacterium amycolatum'' is a gram-positive bacteria, gram-positive, non-spore-forming, aerobic or facultatively anaerobic bacillus capable of fermentation with propionic acid as the major end product of its glucose metabolism. One of its best known relatives is ''Corynebacterium diphtheriae'', the causative agent of diphtheria. ''C. amycolatum'' is a common component of the natural flora found on human skin and mucous membranes, and therefore is an occasional contaminant in human blood cultures but can rarely cause infections such as endocarditis. Identification First described in 1988, ''C. amycolatum'' is one of the diphtheroid most often isolated from clinical samples. However, it is often difficult to differentiate from other fermentative corynebacteria such as ''C. minutissimum'' and ''C. xerosis'', both of which are known human pathogens. One method of differentiation, however, is by observing the cell wall. Unlike other members of this genus, ''C. amycolatum'' lac ...
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Gram-positive Bacteria
In bacteriology, gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their type of cell wall. Gram-positive bacteria take up the crystal violet stain used in the test, and then appear to be purple-coloured when seen through an optical microscope. This is because the thick peptidoglycan layer in the bacterial cell wall retains the stain after it is washed away from the rest of the sample, in the decolorization stage of the test. Conversely, gram-negative bacteria cannot retain the violet stain after the decolorization step; alcohol used in this stage degrades the outer membrane of gram-negative cells, making the cell wall more porous and incapable of retaining the crystal violet stain. Their peptidoglycan layer is much thinner and sandwiched between an inner cell membrane and a bacterial outer membrane, causing them to take up the counterstain ( ...
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