|
Chevron Plot
A chevron plot is a way of representing protein folding chemical kinetics, kinetic data in the presence of varying concentrations of Denaturation (biochemistry), denaturant that disrupts the protein's native tertiary structure. The plot is known as "chevron" plot because of the canonical ''v'', or Chevron (insignia), chevron shape observed when the logarithm of the observed relaxation rate is plotted as a function of the denaturant concentration. In a two-state system, folding and unfolding rates dominate the observed relaxation rates below and above the denaturation midpoint (Cm). This gives rise to the terminology of folding and unfolding arms for the limbs of the chevron. A priori information on the Cm of a protein can be obtained from equilibrium experiments. In fitting to a two-state model, the logarithm of the folding and unfolding rates is assumed to depend linearly on the denaturant concentration, thus resulting in the slopes mf and mu, called the folding and unfolding m-v ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chevron Plot
A chevron plot is a way of representing protein folding chemical kinetics, kinetic data in the presence of varying concentrations of Denaturation (biochemistry), denaturant that disrupts the protein's native tertiary structure. The plot is known as "chevron" plot because of the canonical ''v'', or Chevron (insignia), chevron shape observed when the logarithm of the observed relaxation rate is plotted as a function of the denaturant concentration. In a two-state system, folding and unfolding rates dominate the observed relaxation rates below and above the denaturation midpoint (Cm). This gives rise to the terminology of folding and unfolding arms for the limbs of the chevron. A priori information on the Cm of a protein can be obtained from equilibrium experiments. In fitting to a two-state model, the logarithm of the folding and unfolding rates is assumed to depend linearly on the denaturant concentration, thus resulting in the slopes mf and mu, called the folding and unfolding m-v ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |