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Cycloheximide
Cycloheximide is a naturally occurring fungicide produced by the bacterium '' Streptomyces griseus''. Cycloheximide exerts its effects by interfering with the translocation step in protein synthesis (movement of two tRNA molecules and mRNA in relation to the ribosome), thus blocking eukaryotic translational elongation. Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied ''in vitro'' (''i.e.'' outside of organisms). It is inexpensive and works rapidly. Its effects are rapidly reversed by simply removing it from the culture medium. Due to significant toxic side effects, including DNA damage, teratogenesis, and other reproductive effects (including birth defects and toxicity to sperm), cycloheximide is generally used only in ''in vitro'' research applications, and is not suitable for human use as a therapeutic compound. Although it has been used as a fungicide in agricultural applications, this application is now decreas ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cycloheximide Chase
Cycloheximide chase assays are an experimental technique used in molecular and cellular biology to measure steady state protein stability. Cycloheximide is a drug that inhibits the elongation step in eukaryotic Translation (biology), protein translation, thereby preventing protein synthesis. The addition of cycloheximide to cultured cells followed by protein lysis at multiple timepoints is conducted to observe protein degradation over time and can be used to determine a protein's half-life. These assays are often followed by western blotting to assess protein abundance and can be analyzed using quantitative tools such as ImageJ. Implementation Cycloheximide chase assays have been conducted using a variety of cell types such as yeast and mammalian cell lines. Depending on the cell system used for analysis, the assay may vary in application and time course. For example, yeast cells expressing a protein substrate of interest typically require cycloheximide chases lasting up to 90 min ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Acetoxycycloheximide
Acetoxycycloheximide is an organic chemical compound. It can be considered as the acetylated analogue of cycloheximide. It is a potent protein synthesis inhibitor in animal cells and can inhibit the formation of memories. See also * Cycloheximide Cycloheximide is a naturally occurring fungicide produced by the bacterium '' Streptomyces griseus''. Cycloheximide exerts its effects by interfering with the translocation step in protein synthesis (movement of two tRNA molecules and mRNA in re ... References {{reflist Acetate esters Secondary alcohols Antibiotics Glutarimides Ketones ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Alma Joslyn Whiffen-Barksdale
Alma Joslyn Whiffen-Barksdale (October 25, 1916 – July 5, 1981) was an American mycologist who discovered ''cycloheximide''. She was born in Hammonton, New Jersey. She received a bachelor's degree from Maryville College (1937). Her Masters (botany, 1939) and Ph.D. (botany and mycology, 1941) were earned at the University of North Carolina. In 1941–42. She was a Carnegie Fellow, and in 1951, she was a Guggenheim Fellow. Barksdale worked at the Department of Antibiotic Research of the Upjohn Company of Kalamazoo, Michigan (1943–52) and at the New York Botanical Garden. Barksdale became a foundational figure in the study of '' Achlya'', a genus of aquatic fungi with a unique reproductive system, while working at the New York Botanical Garden; The Mycological Society of America and the ''Achlya Newsletter'', a publication of continuing research on ''Achlya'', both published retrospectives on her life and work following her death in 1981.Achlya Newsletter. (1981). Barksdale, Al ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Translation (genetics)
In biology, translation is the process in living cells in which proteins are produced using RNA molecules as templates. The generated protein is a sequence of amino acids. This sequence is determined by the sequence of nucleotides in the RNA. The nucleotides are considered three at a time. Each such triple results in the addition of one specific amino acid to the protein being generated. The matching from nucleotide triple to amino acid is called the genetic code. The translation is performed by a large complex of functional RNA and proteins called ribosomes. The entire process is called gene expression. In translation, messenger RNA (mRNA) is decoded in a ribosome, outside the nucleus, to produce a specific amino acid chain, or polypeptide. The polypeptide later folds into an active protein and performs its functions in the cell. The polypeptide can also start folding during protein synthesis. The ribosome facilitates decoding by inducing the binding of complementary transfe ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Upjohn
The Upjohn Company was an American pharmaceutical manufacturing firm (est. 1886) in Hastings, Michigan, by Dr. William E. Upjohn, an 1875 graduate of the University of Michigan medical school. The company was originally formed to make ''friable pills'', specifically designed to crush easily, and thus be easier for patients to digest. Upjohn initially marketed the pills to doctors by sending them a wooden plank along with a rival’s pill and one of Upjohn’s, with instructions to try to hammer the pills into the plank. History Upjohn developed a process for the large scale production of cortisone. The oxygen atom group must be in position 11 for this steroid to function. There are, however, no known natural starting materials with an oxo-group in position 11. The only method for preparing cortisone prior to 1952 was a lengthy synthesis, starting from cholic acid isolated from bile. In 1952, two Upjohn biochemists, Dury Peterson and Herb Murray, announced that they had invente ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fungicides
Fungicides are pesticides used to kill parasitic fungi or their spores. Fungi can cause serious damage in agriculture, resulting in losses of yield and quality. Fungicides are used both in agriculture and to fight fungal infections in animals, including humans. Fungicides are also used to control oomycetes, which are not taxonomically/genetically fungi, although sharing similar methods of infecting plants. Fungicides can either be contact, translaminar or systemic. Contact fungicides are not taken up into the plant tissue and protect only the plant where the spray is deposited. Translaminar fungicides redistribute the fungicide from the upper, sprayed leaf surface to the lower, unsprayed surface. Systemic fungicides are taken up and redistributed through the xylem vessels. Few fungicides move to all parts of a plant. Some are locally systemic, and some move upward. Most fungicides that can be bought retail are sold in liquid form, the active ingredient being present at 0.08% i ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Protein Synthesis Inhibitors
A protein synthesis inhibitor is a compound that stops or slows the growth or proliferation of cells by disrupting the processes that lead directly to the generation of new proteins. While a broad interpretation of this definition could be used to describe nearly any compound depending on concentration, in practice, it usually refers to compounds that act at the molecular level on translational machinery (either the ribosome itself or the translation factor), taking advantages of the major differences between prokaryotic and eukaryotic ribosome structures. Mechanism In general, protein synthesis inhibitors work at different stages of bacterial mRNA translation into proteins, like initiation, elongation (including aminoacyl tRNA entry, proofreading, peptidyl transfer, and bacterial translocation) and termination: Earlier stages * Rifamycin inhibits bacterial DNA transcription into mRNA by inhibiting DNA-dependent RNA polymerase by binding its beta-subunit. * alpha-Amanitin i ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Secondary Alcohols
In chemistry, an alcohol (), is a type of organic compound that carries at least one hydroxyl () functional group bound to a saturated carbon atom. Alcohols range from the simple, like methanol and ethanol, to complex, like sugar alcohols and cholesterol. The presence of an OH group strongly modifies the properties of hydrocarbons, conferring hydrophilic (water-loving) properties. The OH group provides a site at which many reactions can occur. History The flammable nature of the exhalations of wine was already known to ancient natural philosophers such as Aristotle (384–322 BCE), Theophrastus (–287 BCE), and Pliny the Elder (23/24–79 CE). However, this did not immediately lead to the isolation of alcohol, even despite the development of more advanced distillation techniques in second- and third-century Roman Egypt. An important recognition, first found in one of the writings attributed to Jābir ibn Ḥayyān (ninth century CE), was that by adding salt to boiling win ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Athlete's Foot
Athlete's foot, known medically as ''tinea pedis'', is a common skin infection of the feet caused by a fungus. Signs and symptoms often include itching, scaling, cracking and redness. In rare cases the skin may blister. Athlete's foot fungus may infect any part of the foot, but most often grows between the toes. The next most common area is the bottom of the foot. The same fungus may also affect the nails or the hands. It is a member of the group of diseases known as '' tinea''. Athlete's foot is caused by a number of different funguses, including species of '' Trichophyton'', '' Epidermophyton'', and '' Microsporum''. The condition is typically acquired by coming into contact with infected skin, or fungus in the environment. Common places where the funguses can survive are around swimming pools and in locker rooms. They may also be spread from other animals. Usually diagnosis is made based on signs and symptoms; however, it can be confirmed either by culture or seeing hy ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Neoscytalidium Dimidiatum
''Neoscytalidium dimidiatum'' was first described in 1933 as ''Hendersonula toruloidea'' from diseased orchard trees in Egypt. Decades later, it was determined to be a causative agent of human dermatomycosis-like infections and foot infections predominantly in tropical areas; however the fungus is considered to be widespread. A newer name, ''Scytalidium dimidiatum'', was applied to a synanamorph of ''Nattrassia mangiferae'', otherwise known as ''Neofusicoccum mangiferae''. Substantial confusion has arisen in the literature on this fungus resulting from the use of multiple different names including ''Torula dimidiata'', ''Fusicoccum dimidiatum'', ''Scytalidium dimidiatum'', and ''Hendersonula toruloidea''. Additionally, ''Scytalidium lignicola'' and ''Scytalidium lignicolum'' are often considered earlier names of ''N. dimidiatum''. History and taxonomy In 1933, British mycologist Dr. Rolland Marshall Nattrass described an arthroconidial asexual fungus that he named ''H. toruloidea ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Saccharomyces Cerevisiae
''Saccharomyces cerevisiae'' () (brewer's yeast or baker's yeast) is a species of yeast (single-celled fungal microorganisms). The species has been instrumental in winemaking, baking, and brewing since ancient times. It is believed to have been originally isolated from the skin of grapes. It is one of the most intensively studied eukaryotic model organisms in molecular and cell biology, much like '' Escherichia coli'' as the model bacterium. It is the microorganism which causes many common types of fermentation. ''S. cerevisiae'' cells are round to ovoid, 5–10 μm in diameter. It reproduces by budding. Many proteins important in human biology were first discovered by studying their homologs in yeast; these proteins include cell cycle proteins, signaling proteins, and protein-processing enzymes. ''S. cerevisiae'' is currently the only yeast cell known to have Berkeley bodies present, which are involved in particular secretory pathways. Antibodies again ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mycosphaerella Graminicola
''Zymoseptoria tritici'', synonyms ''Septoria tritici'', ''Mycosphaerella graminicola'', is a species of filamentous fungus, an ascomycete in the family '' Mycosphaerellaceae''. It is a wheat plant pathogen causing septoria leaf blotch that is difficult to control due to resistance to multiple fungicides. The pathogen today causes one of the most important diseases of wheat. In 2011, Quaedvlieg et al. introduced a new combination for this species: ''Zymoseptoria tritici'', as they found that the type strains of both the genus ''Mycosphaerella'' (linked to the anamorph genus ''Ramularia'') and the genus ''Septoria'' (linked to the genus ''Septoria'', an extensive clade of very distinct septoria-like species within the ''Mycosphaerellaceae'') clustered separately from the clade containing both ''Zymoseptoria tritici'' and ''Z. passerinii''. Since 2011, a total of eight ''Zymoseptoria'' species have been described within the genus ''Zymoseptoria''; ''Z. tritici'' (the type of th ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
