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Chemostat Shematic
A chemostat (from ''chem''ical environment is ''stat''ic) is a bioreactor to which fresh medium is continuously added, while culture liquid containing left over nutrients, metabolic end products and microorganisms is continuously removed at the same rate to keep the culture volume constant. By changing the rate with which medium is added to the bioreactor the specific growth rate of the microorganism can be easily controlled within limits. Operation Steady state One of the most important features of chemostats is that microorganisms can be grown in a physiological steady state under constant environmental conditions. In this steady state, growth occurs at a constant specific growth rate and all culture parameters remain constant (culture volume, dissolved oxygen concentration, nutrient and product concentrations, pH, cell density, etc.). In addition, environmental conditions can be controlled by the experimenter. Microorganisms growing in chemostats usually reach a steady stat ...
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Chemostat Shematic
A chemostat (from ''chem''ical environment is ''stat''ic) is a bioreactor to which fresh medium is continuously added, while culture liquid containing left over nutrients, metabolic end products and microorganisms is continuously removed at the same rate to keep the culture volume constant. By changing the rate with which medium is added to the bioreactor the specific growth rate of the microorganism can be easily controlled within limits. Operation Steady state One of the most important features of chemostats is that microorganisms can be grown in a physiological steady state under constant environmental conditions. In this steady state, growth occurs at a constant specific growth rate and all culture parameters remain constant (culture volume, dissolved oxygen concentration, nutrient and product concentrations, pH, cell density, etc.). In addition, environmental conditions can be controlled by the experimenter. Microorganisms growing in chemostats usually reach a steady stat ...
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Ecology
Ecology () is the study of the relationships between living organisms, including humans, and their physical environment. Ecology considers organisms at the individual, population, community, ecosystem, and biosphere level. Ecology overlaps with the closely related sciences of biogeography, evolutionary biology, genetics, ethology, and natural history. Ecology is a branch of biology, and it is not synonymous with environmentalism. Among other things, ecology is the study of: * The abundance, biomass, and distribution of organisms in the context of the environment * Life processes, antifragility, interactions, and adaptations * The movement of materials and energy through living communities * The successional development of ecosystems * Cooperation, competition, and predation within and between species * Patterns of biodiversity and its effect on ecosystem processes Ecology has practical applications in conservation biology, wetland management, natural resource managemen ...
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Auxostat
An auxostat is a continuous culture device which, while in operation, uses feedback from a measurement taken on the growth chamber to control the media flow rate, maintaining the measurement at a constant. Auxo was the Greek goddess of spring growth, and represents nutrients as a prefix. However, the most typical auxostats are pH-auxostats, with feedback between the growth rate and a pH meter. Other auxostats may measure oxygen tension, ethanol concentrations, and sugar Sugar is the generic name for sweet-tasting, soluble carbohydrates, many of which are used in food. Simple sugars, also called monosaccharides, include glucose, fructose, and galactose. Compound sugars, also called disaccharides or double ... concentrations References {{Reflist Bioreactors ...
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Turbidostat
A turbidostat is a continuous microbiological culture device, similar to a chemostat or an auxostat, which has feedback between the turbidity of the culture vessel and the dilution rate. The theoretical relationship between growth in a chemostat and growth in a turbidostat is somewhat complex, in part because they are similar. A chemostat A chemostat (from ''chem''ical environment is ''stat''ic) is a bioreactor to which fresh medium is continuously added, while culture liquid containing left over nutrients, metabolic end products and microorganisms is continuously removed at the sa ... has a fixed volume and flow rate, and thus a fixed dilution rate. A turbidostat dynamically adjusts the flow rate (and therefore the dilution rate) to make the turbidity constant. At steady state, operation of both the chemostat and turbidostat are identical. It is only when classical chemostat assumptions are violated (for instance, out of equilibrium; or the cells are mutating) that a turbido ...
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Mutation
In biology, a mutation is an alteration in the nucleic acid sequence of the genome of an organism, virus, or extrachromosomal DNA. Viral genomes contain either DNA or RNA. Mutations result from errors during DNA or viral replication, mitosis, or meiosis or other types of damage to DNA (such as pyrimidine dimers caused by exposure to ultraviolet radiation), which then may undergo error-prone repair (especially microhomology-mediated end joining), cause an error during other forms of repair, or cause an error during replication (translesion synthesis). Mutations may also result from insertion or deletion of segments of DNA due to mobile genetic elements. Mutations may or may not produce detectable changes in the observable characteristics (phenotype) of an organism. Mutations play a part in both normal and abnormal biological processes including: evolution, cancer, and the development of the immune system, including junctional diversity. Mutation is the ultimate source o ...
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Single-nucleotide Polymorphism
In genetics, a single-nucleotide polymorphism (SNP ; plural SNPs ) is a germline substitution of a single nucleotide at a specific position in the genome. Although certain definitions require the substitution to be present in a sufficiently large fraction of the population (e.g. 1% or more), many publications do not apply such a frequency threshold. For example, at a specific base position in the human genome, the G nucleotide may appear in most individuals, but in a minority of individuals, the position is occupied by an A. This means that there is a SNP at this specific position, and the two possible nucleotide variations – G or A – are said to be the alleles for this specific position. SNPs pinpoint differences in our susceptibility to a wide range of diseases, for example age-related macular degeneration (a common SNP in the CFH gene is associated with increased risk of the disease) or nonalcoholic fatty liver disease (a SNP in the PNPLA3 gene is associated with inc ...
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Appl
Benjamin Appl (born 26 June 1982) is a German-British lyric baritone, a classical singer who has appeared world-wide in opera houses and concert halls, particularly known as a Lieder singer. Early life and education Born in Regensburg, Appl has two older brothers, with whom he sang as a chorister with the Regensburger Domspatzen, the boys' choir at the Regensburg Cathedral, performing in concerts across Europe and Asia. Following secondary school, rather than spending a compulsory year in the army he completed his alternative community service working for the Bayerischer Blinden- und Sehbehindertenbund in Regensburg, providing assistance to blind people in the community. He started training as a bank clerk at the Liga Bank in Regensburg. He then studied business administration at the University of Regensburg, graduating in 2009 with a diploma. His diploma thesis, an empirical study of withdrawn initial public offerings, received the highest possible marks. He was awarded a scho ...
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Biofilm
A biofilm comprises any syntrophic consortium of microorganisms in which cells stick to each other and often also to a surface. These adherent cells become embedded within a slimy extracellular matrix that is composed of extracellular polymeric substances (EPSs). The cells within the biofilm produce the EPS components, which are typically a polymeric conglomeration of extracellular polysaccharides, proteins, lipids and DNA. Because they have three-dimensional structure and represent a community lifestyle for microorganisms, they have been metaphorically described as "cities for microbes". Biofilms may form on living or non-living surfaces and can be prevalent in natural, industrial, and hospital settings. They may constitute a microbiome or be a portion of it. The microbial cells growing in a biofilm are physiologically distinct from planktonic cells of the same organism, which, by contrast, are single cells that may float or swim in a liquid medium. Biofilms can form ...
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Silane
Silane is an inorganic compound with chemical formula, . It is a colourless, pyrophoric, toxic gas with a sharp, repulsive smell, somewhat similar to that of acetic acid. Silane is of practical interest as a precursor to elemental silicon. Silane with alkyl groups are effective water repellents for mineral surfaces such as concrete and masonry. Silanes with both organic and inorganic attachments are used as coupling agents. Production Commercial-scale routes Silane can be produced by several routes. Typically, it arises from the reaction of hydrogen chloride with magnesium silicide: : Mg2Si + 4 HCl -> 2 MgCl2 + SiH4 It is also prepared from metallurgical-grade silicon in a two-step process. First, silicon is treated with hydrogen chloride at about 300 °C to produce trichlorosilane, HSiCl3, along with hydrogen gas, according to the chemical equation : Si + 3 HCl -> HSiCl3 + H2 The trichlorosilane is then converted to a mixture of silane and silicon tetrachloride: : 4 HS ...
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Aeration
Aeration (also called aerification or aeriation) is the Systems engineering process, process by which air is circulated through, mixed with or solvation, dissolved in a liquid or other substances that act as a fluid (such as soil). Aeration processes create additional surface area in the mixture, allowing greater chemical or suspension reactions. Aeration of liquids Methods Aeration of liquids (usually water) is achieved by: * passing air through the liquid by means of the Venturi tube, Aeration Turbine, aeration turbines or compressed air which can be combined with diffuser(s) air stone(s), as well as fine bubble diffusers, coarse bubble diffusers or linear aeration tubing. Ceramics are suitable for this purpose, often involving dispersion of fine air or gas bubbles through the porous ceramic into a liquid. The smaller the bubbles, the more gas is exposed to the liquid increasing the gas transfer efficiency. Diffusers or spargers can also be designed into the system to cause turbu ...
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