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Cell Potency
Pluripotency: These are the cells that can generate into any of the three Germ layers which imply Endodermal, Mesodermal, and Ectodermal cells except tissues like the placenta. According to Latin terms, Pluripotentia means the ability for many things. We can generate Induced Pluripotent cells by using the Induced pluripotency technique by triggering or expressing the genes or the transcription factors of the normal somatic cells. They are abbreviated as iPSC or IPS. We can forcefully express the transcription factors like Oct4, Sox2, Klf4, and c-Myc of a non-pluripotent cell and convert them into a stem cell. This procedure is first studied in a Mouse fibroblast cell in 2006 and followed the same instructions in developing a Human pluripotent cell from a Human epidermal fibroblast cell. The technique is called Regeneration. Though the iPSC has similar properties to embryonic stem cells they were never approved for clinical stage research because they are highly Tumerogenic, hav ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Oct4
Oct-4 (octamer-binding transcription factor 4), also known as POU5F1 (POU domain, class 5, transcription factor 1), is a protein that in humans is encoded by the ''POU5F1'' gene. Oct-4 is a homeodomain transcription factor of the POU family. It is critically involved in the self-renewal of undifferentiated embryonic stem cells. As such, it is frequently used as a marker for undifferentiated cells. Oct-4 expression must be closely regulated; too much or too little will cause differentiation of the cells. Octamer-binding transcription factor 4, OCT-4, is a transcription factor protein that is encoded by the ''POU5F1'' gene and is part of the POU (Pit-Oct-Unc) family. OCT-4 consists of an octamer motif, a particular DNA sequence of AGTCAAAT that binds to their target genes and activates or deactivates certain expressions. These gene expressions then lead to phenotypic changes in stem cell differentiation during the development of a mammalian embryo. It plays a vital role in det ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Blastomere
In biology, a blastomere is a type of cell produced by cell division (cleavage) of the zygote after fertilization; blastomeres are an essential part of blastula formation, and blastocyst formation in mammals. Human blastomere characteristics In humans, blastomere formation begins immediately following fertilization and continues through the first week of embryonic development. About 90 minutes after fertilization, the zygote divides into two cells. The two-cell blastomere state, present after the zygote first divides, is considered the earliest mitotic product of the fertilized oocyte. These mitotic divisions continue and result in a grouping of cells called blastomeres. During this process, the total size of the embryo does not increase, so each division results in smaller and smaller cells. When the zygote contains 16 to 32 blastomeres it is referred to as a morula. These are the preliminary stages in the embryo beginning to form. Once this begins, microtubules within the mor ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Inner Cell Mass
The inner cell mass (ICM) or embryoblast (known as the pluriblast in marsupials) is a structure in the early development of an embryo. It is the mass of cells inside the blastocyst that will eventually give rise to the definitive structures of the fetus. The inner cell mass forms in the earliest stages of embryonic development, before implantation into the endometrium of the uterus. The ICM is entirely surrounded by the single layer of trophoblast cells of the trophectoderm. Further development The physical and functional separation of the inner cell mass from the trophectoderm (TE) is a special feature of mammalian development and is the first cell lineage specification in these embryos. Following fertilization in the oviduct, the mammalian embryo undergoes a relatively slow round of cleavages to produce an eight-cell morula. Each cell of the morula, called a blastomere, increases surface contact with its neighbors in a process called compaction. This results in a pola ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Blastocyst
The blastocyst is a structure formed in the early embryonic development of mammals. It possesses an inner cell mass (ICM) also known as the ''embryoblast'' which subsequently forms the embryo, and an outer layer of trophoblast cells called the trophectoderm. This layer surrounds the inner cell mass and a fluid-filled cavity known as the blastocoel. In the late blastocyst the trophectoderm is known as the trophoblast. The trophoblast gives rise to the chorion and amnion, the two fetal membranes that surround the embryo. The placenta derives from the embryonic chorion (the portion of the chorion that develops villi) and the underlying uterine tissue of the mother. The name "blastocyst" arises from the Greek ' ("a sprout") and ' ("bladder, capsule"). In other animals this is a structure consisting of an undifferentiated ball of cells and is called a blastula. In humans, blastocyst formation begins about five days after fertilization when a fluid-filled cavity opens up in the mor ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Morula
A morula (Latin, ''morus'': mulberry) is an early-stage embryo consisting of a solid ball of cells called blastomeres, contained in mammals, and other animals within the zona pellucida shell. The blastomeres are the daughter cells of the zygote, and when the blastomeres number from 16–32 the ball of cells is called a morula. At the 8-cell stage the blastomeres are round and only loosely adhered. With further division they begin to become flattened, and develop an inside-out polarity that optimises the cell to cell contact. In the human embryo by day four after fertilisation, the morula at about the 32–cell stage begins to take in fluid. The fluid comes about from sodium-potassium pumps (on trophoblasts) that pump sodium into the morula, drawing in water from the maternal environment to become blastocoelic fluid. Hydrostatic pressure of the fluid creates a large cavity in the morula called a blastocoel or blastocyst cavity. Embryoblast cells also known as the inner cell mas ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Syncytiotrophoblast
Syncytiotrophoblast (from the Greek 'syn'- "together"; 'cytio'- "of cells"; 'tropho'- "nutrition"; 'blast'- "bud") is the epithelial covering of the highly vascular embryonic placental villi, which invades the wall of the uterus to establish nutrient circulation between the embryo and the mother. It is a multi-nucleate, terminally differentiated syncytium, extending to 13cm. Function It is the outer layer of the trophoblasts and actively invades the uterine wall, during implantation, rupturing maternal capillaries and thus establishing an interface between maternal blood and embryonic extracellular fluid, facilitating passive exchange of material between the mother and the embryo. The syncytial property is important since the mother's immune system includes white blood cells that are able to migrate into tissues by "squeezing" in between cells. If they were to reach the fetal side of the placenta many foreign proteins would be recognised, triggering an immune reaction. Howeve ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cytotrophoblast
"Cytotrophoblast" is the name given to both the inner layer of the trophoblast (also called layer of Langhans) or the cells that live there. It is interior to the syncytiotrophoblast and external to the wall of the blastocyst in a developing embryo. The cytotrophoblast is considered to be the trophoblastic stem cell because the layer surrounding the blastocyst remains while daughter cells differentiate and proliferate to function in multiple roles. There are two lineages that cytotrophoblastic cells may differentiate through: fusion and invasive. The fusion lineage yields syncytiotrophoblast and the invasive lineage yields interstitial cytotrophoblast cells. Cytotrophoblastic cells play an important role in the implantation of an embryo in the uterus. Fusion lineage The formation of all syncytiotrophoblast is from the fusion of two or more cytotrophoblasts via this fusion pathway. This pathway is important because the syncytiotrophoblast plays an important role in fetal-maternal ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Placenta
The placenta is a temporary embryonic and later fetal organ that begins developing from the blastocyst shortly after implantation. It plays critical roles in facilitating nutrient, gas and waste exchange between the physically separate maternal and fetal circulations, and is an important endocrine organ, producing hormones that regulate both maternal and fetal physiology during pregnancy. The placenta connects to the fetus via the umbilical cord, and on the opposite aspect to the maternal uterus in a species-dependent manner. In humans, a thin layer of maternal decidual (endometrial) tissue comes away with the placenta when it is expelled from the uterus following birth (sometimes incorrectly referred to as the 'maternal part' of the placenta). Placentas are a defining characteristic of placental mammals, but are also found in marsupials and some non-mammals with varying levels of development. Mammalian placentas probably first evolved about 150 million to 200 million years ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Ectoderm
The ectoderm is one of the three primary germ layers formed in early embryonic development. It is the outermost layer, and is superficial to the mesoderm (the middle layer) and endoderm (the innermost layer). It emerges and originates from the outer layer of germ cells. The word ectoderm comes from the Greek ''ektos'' meaning "outside", and ''derma'' meaning "skin".Gilbert, Scott F. Developmental Biology. 9th ed. Sunderland, MA: Sinauer Associates, 2010: 333-370. Print. Generally speaking, the ectoderm differentiates to form epithelial and neural tissues (spinal cord, peripheral nerves and brain). This includes the skin, linings of the mouth, anus, nostrils, sweat glands, hair and nails, and tooth enamel. Other types of epithelium are derived from the endoderm. In vertebrate embryos, the ectoderm can be divided into two parts: the dorsal surface ectoderm also known as the external ectoderm, and the neural plate, which invaginates to form the neural tube and neural crest. Th ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mesoderm
The mesoderm is the middle layer of the three germ layers that develops during gastrulation in the very early development of the embryo of most animals. The outer layer is the ectoderm, and the inner layer is the endoderm.Langman's Medical Embryology, 11th edition. 2010. The mesoderm forms mesenchyme, mesothelium, non-epithelial blood cells and coelomocytes. Mesothelium lines coeloms. Mesoderm forms the muscles in a process known as myogenesis, septa (cross-wise partitions) and mesenteries (length-wise partitions); and forms part of the gonads (the rest being the gametes). Myogenesis is specifically a function of mesenchyme. The mesoderm differentiates from the rest of the embryo through intercellular signaling, after which the mesoderm is polarized by an organizing center. The position of the organizing center is in turn determined by the regions in which beta-catenin is protected from degradation by GSK-3. Beta-catenin acts as a co-factor that alters the activity of ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Endoderm
Endoderm is the innermost of the three primary germ layers in the very early embryo. The other two layers are the ectoderm (outside layer) and mesoderm (middle layer). Cells migrating inward along the archenteron form the inner layer of the gastrula, which develops into the endoderm. The endoderm consists at first of flattened cells, which subsequently become columnar. It forms the epithelial lining of multiple systems. In plant biology, endoderm corresponds to the innermost part of the cortex ( bark) in young shoots and young roots often consisting of a single cell layer. As the plant becomes older, more endoderm will lignify. Production The following chart shows the tissues produced by the endoderm. The embryonic endoderm develops into the interior linings of two tubes in the body, the digestive and respiratory tube. Liver and pancreas cells are believed to derive from a common precursor. In humans, the endoderm can differentiate into distinguishable organs after 5 week ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Spermatozoon
A spermatozoon (; also spelled spermatozoön; ; ) is a motile sperm cell, or moving form of the haploid cell that is the male gamete. A spermatozoon joins an ovum to form a zygote. (A zygote is a single cell, with a complete set of chromosomes, that normally develops into an embryo.) Sperm cells contribute approximately half of the nuclear genetic information to the diploid offspring (excluding, in most cases, mitochondrial DNA). In mammals, the sex of the offspring is determined by the sperm cell: a spermatozoon bearing an X chromosome will lead to a female (XX) offspring, while one bearing a Y chromosome will lead to a male (XY) offspring. Sperm cells were first observed in Antonie van Leeuwenhoek's laboratory in 1677. Mammalian spermatozoon structure, function, and size Humans The human sperm cell is the reproductive cell in males and will only survive in warm environments; once it leaves the male body the sperm's survival likelihood is reduced and it may die, thereby dec ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
