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Blunt End
Blunt end may refer to: * Sticky and blunt ends * The flat end cab of the British Rail Class 91 The British Rail Class 91 is a high-speed rail, high-speed electric locomotive, which produces power of ; it was ordered as a component of the East Coast Main Line modernisation and electrification programme of the late 1980s. The Class 91s wer ...
, commonly called the blunt end cab {{disambig ...
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Sticky And Blunt Ends
DNA ends refer to the properties of the ends of linear DNA molecules, which in molecular biology are described as "sticky" or "blunt" based on the shape of the complementary strands at the terminus. In sticky ends, one strand is longer than the other (typically by at least a few nucleotides), such that the longer strand has bases which are left unpaired. In blunt ends, both strands are of equal length – i.e. they end at the same base position, leaving no unpaired bases on either strand. The concept is used in molecular biology, in cloning, or when subcloning insert DNA into vector DNA. Such ends may be generated by restriction enzymes that break the molecule's phosphodiester backbone at specific locations, which themselves belong to a larger class of enzymes called exonucleases and endonucleases. A restriction enzyme that cuts the backbones of both strands at non-adjacent locations leaves a staggered cut, generating two overlapping sticky ends, while an enzyme that makes a str ...
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