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Biological Stain Commission
The Biological Stain Commission (BSC) is an organization that provides third-party testing and certification of dyes and a few other compounds that are used to enhance contrast in specimens examined in biological and medical laboratories. The BSC is a 95-year-old organization well known to many thousands of scientists, worldwide but especially in N America, who buy BSC-certified stains for staining microscopic preparations and for making selective culture media for bacteria. Manufacturers and other vendors submit samples from their batches of dyes to the BSC's independent laboratory in Rochester NY. The BSC's certification label on a bottle of dye indicates that the contents are from a batch that passed the tests for chemical purity and for efficacy as a biological stain. These tests are published (Penney et al. 2002a). Changes to tests and additions to the list of stains eligible for certification are published from time to time in ''Biotechnic & Histochemistry'' and are summari ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Staining
Staining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently used in histology (microscopic study of biological tissues), in cytology (microscopic study of cells), and in the medical fields of histopathology, hematology, and cytopathology that focus on the study and diagnoses of diseases at the microscopic level. Stains may be used to define biological tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells), or organelles within individual cells. In biochemistry, it involves adding a class-specific ( DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis. Light microscopes are used for viewin ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Microscopy
Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy. Optical microscopy and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron beams interacting with the specimen, and the collection of the scattered radiation or another signal in order to create an image. This process may be carried out by wide-field irradiation of the sample (for example standard light microscopy and transmission electron microscopy) or by scanning a fine beam over the sample (for example confocal laser scanning microscopy and scanning electron microscopy). Scanning probe microscopy involves the interaction of a scanning probe with the surface of the objec ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Growth Medium
A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation or small plants like the moss ''Physcomitrella patens''. Different types of media are used for growing different types of cells. The two major types of growth media are those used for cell culture, which use specific cell types derived from plants or animals, and those used for microbiological culture, which are used for growing microorganisms such as bacteria or fungi. The most common growth media for microorganisms are nutrient broths and agar plates; specialized media are sometimes required for microorganism and cell culture growth. Some organisms, termed fastidious organisms, require specialized environments due to complex nutritional requirements. Viruses, for example, are obligate intracellular parasites and require a growth medium containing living cells. Types The most common growth media ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Biotechnic & Histochemistry
''Biotechnic & Histochemistry'' is a peer-reviewed scientific journal that covers all aspects of histochemistry and microtechnic in the biological sciences from botany to cell biology to medicine. It is published by Taylor & Francis on behalf of the Biological Stain Commission. The journal was established in 1926 as ''Stain Technology'' by Harold J. Conn. It obtained its current title in 1991. The editor-in-chief is G. S. Nettleton. Abstracting and indexing The journal is abstracted and indexed in Index Medicus/MEDLINE/PubMed, Current Contents/Life Sciences, Science Citation Index, and EMBASE/Excerpta Medica. According to the ''Journal Citation Reports'', the journal has a 2020 impact factor The impact factor (IF) or journal impact factor (JIF) of an academic journal is a scientometric index calculated by Clarivate that reflects the yearly mean number of citations of articles published in the last two years in a given journal, as i ... of 1.718. References External li ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Harold J
Harold may refer to: People * Harold (given name), including a list of persons and fictional characters with the name * Harold (surname), surname in the English language * András Arató, known in meme culture as "Hide the Pain Harold" Arts and entertainment * Harold (film), ''Harold'' (film), a 2008 comedy film * ''Harold'', an 1876 poem by Alfred, Lord Tennyson * ''Harold, the Last of the Saxons'', an 1848 book by Edward Bulwer-Lytton, 1st Baron Lytton * ''Harold or the Norman Conquest'', an opera by Frederic Cowen * ''Harold'', an 1885 opera by Eduard Nápravník * Harold, a character from the cartoon List of The Grim Adventures of Billy & Mandy characters#Harold, ''The Grim Adventures of Billy & Mandy'' *Harold & Kumar, a US movie; Harold/Harry is the main actor in the show. Places ;In the United States * Alpine, Los Angeles County, California, an erstwhile settlement that was also known as Harold * Harold, Florida, an unincorporated community * Harold, Kentucky, an ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Histochemistry
Immunohistochemistry (IHC) is the most common application of immunostaining. It involves the process of selectively identifying antigens (proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. IHC takes its name from the roots "immuno", in reference to antibodies used in the procedure, and "histo", meaning tissue (compare to immunocytochemistry). Albert Coons conceptualized and first implemented the procedure in 1941. Visualising an antibody-antigen interaction can be accomplished in a number of ways, mainly either of the following: * ''Chromogenic immunohistochemistry'' (CIH), wherein an antibody is conjugated to an enzyme, such as peroxidase (the combination being termed immunoperoxidase), that can catalyse a colour-producing reaction. * '' Immunofluorescence'', where the antibody is tagged to a fluorophore, such as fluorescein or rhodamine. Immunohistochemical staining is widely used in the diag ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Histology
Histology, also known as microscopic anatomy or microanatomy, is the branch of biology which studies the microscopic anatomy of biological tissues. Histology is the microscopic counterpart to gross anatomy, which looks at larger structures visible without a microscope. Although one may divide microscopic anatomy into ''organology'', the study of organs, ''histology'', the study of tissues, and ''cytology'', the study of cells, modern usage places all of these topics under the field of histology. In medicine, histopathology is the branch of histology that includes the microscopic identification and study of diseased tissue. In the field of paleontology, the term paleohistology refers to the histology of fossil organisms. Biological tissues Animal tissue classification There are four basic types of animal tissues: muscle tissue, nervous tissue, connective tissue, and epithelial tissue. All animal tissues are considered to be subtypes of these four principal tissue types ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Histotechnology
Histology, also known as microscopic anatomy or microanatomy, is the branch of biology which studies the microscopic anatomy of biological tissues. Histology is the microscopic counterpart to gross anatomy, which looks at larger structures visible without a microscope. Although one may divide microscopic anatomy into ''organology'', the study of organs, ''histology'', the study of tissues, and ''cytology'', the study of cells, modern usage places all of these topics under the field of histology. In medicine, histopathology is the branch of histology that includes the microscopic identification and study of diseased tissue. In the field of paleontology, the term paleohistology refers to the histology of fossil organisms. Biological tissues Animal tissue classification There are four basic types of animal tissues: muscle tissue, nervous tissue, connective tissue, and epithelial tissue. All animal tissues are considered to be subtypes of these four principal tissue types (for ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Microscopy Organizations
Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy. Optical microscopy and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron beams interacting with the specimen, and the collection of the scattered radiation or another signal in order to create an image. This process may be carried out by wide-field irradiation of the sample (for example standard light microscopy and transmission electron microscopy) or by scanning a fine beam over the sample (for example confocal laser scanning microscopy and scanning electron microscopy). Scanning probe microscopy involves the interaction of a scanning probe with the surface of the object ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Microbiological Media
Microbiology () is the branches of science, scientific study of microorganisms, those being unicellular organism, unicellular (single cell), multicellular organism, multicellular (cell colony), or non-cellular life, acellular (lacking cells). Microbiology encompasses numerous sub-disciplines including virology, bacteriology, protistology, mycology, immunology, and parasitology. eukaryote, Eukaryotic microorganisms possess membrane-bound organelles and include fungi and protists, whereas prokaryote, prokaryotic organisms—all of which are microorganisms—are conventionally classified as lacking membrane-bound organelles and include Bacteria and Archaea. Microbiologists traditionally relied on culture, staining, and microscopy. However, less than 1% of the microorganisms present in common environments can be cultured in isolation using current means. Microbiologists often rely on molecular biology tools such as DNA sequence based identification, for example the 16S ribosomal RNA, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Staining
Staining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently used in histology (microscopic study of biological tissues), in cytology (microscopic study of cells), and in the medical fields of histopathology, hematology, and cytopathology that focus on the study and diagnoses of diseases at the microscopic level. Stains may be used to define biological tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells), or organelles within individual cells. In biochemistry, it involves adding a class-specific ( DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis. Light microscopes are used for viewin ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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