Azotobacter
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Azotobacter
''Azotobacter'' is a genus of usually motile, oval or spherical bacteria that form thick-walled cysts (and also has hard crust) and may produce large quantities of capsular slime. They are aerobic, free-living soil microbes that play an important role in the nitrogen cycle in nature, binding atmospheric nitrogen, which is inaccessible to plants, and releasing it in the form of ammonium ions into the soil (nitrogen fixation). In addition to being a model organism for studying diazotrophs, it is used by humans for the production of biofertilizers, food additives, and some biopolymers. The first representative of the genus, ''Azotobacter chroococcum'', was discovered and described in 1901 by Dutch microbiologist and botanist Martinus Beijerinck. ''Azotobacter'' species are Gram-negative bacteria found in neutral and alkaline soils, in water, and in association with some plants. Biological characteristics Morphology Cells of the genus ''Azotobacter'' are relatively large ...
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Azotobacter Agilis
''Azotobacter'' is a genus of usually motile, oval or spherical bacteria that form thick-walled cysts (and also has hard crust) and may produce large quantities of capsular slime. They are aerobic, free-living soil microbes that play an important role in the nitrogen cycle in nature, binding atmospheric nitrogen, which is inaccessible to plants, and releasing it in the form of ammonium ions into the soil ( nitrogen fixation). In addition to being a model organism for studying diazotrophs, it is used by humans for the production of biofertilizers, food additives, and some biopolymers. The first representative of the genus, '' Azotobacter chroococcum'', was discovered and described in 1901 by Dutch microbiologist and botanist Martinus Beijerinck. ''Azotobacter'' species are Gram-negative bacteria found in neutral and alkaline soils, in water, and in association with some plants. Biological characteristics Morphology Cells of the genus ''Azotobacter'' are relatively large f ...
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Azotobacter Beijerinckii
''Azotobacter'' is a genus of usually motile, oval or spherical bacteria that form thick-walled cysts (and also has hard crust) and may produce large quantities of capsular slime. They are aerobic, free-living soil microbes that play an important role in the nitrogen cycle in nature, binding atmospheric nitrogen, which is inaccessible to plants, and releasing it in the form of ammonium ions into the soil ( nitrogen fixation). In addition to being a model organism for studying diazotrophs, it is used by humans for the production of biofertilizers, food additives, and some biopolymers. The first representative of the genus, '' Azotobacter chroococcum'', was discovered and described in 1901 by Dutch microbiologist and botanist Martinus Beijerinck. ''Azotobacter'' species are Gram-negative bacteria found in neutral and alkaline soils, in water, and in association with some plants. Biological characteristics Morphology Cells of the genus ''Azotobacter'' are relatively large f ...
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Azotobacter Nigricans
''Azotobacter'' is a genus of usually Motility, motile, oval or spherical bacteria that form thick-walled cysts (and also has hard crust) and may produce large quantities of capsular Mucus, slime. They are aerobic, free-living soil Microorganism, microbes that play an important role in the nitrogen cycle in nature, binding atmospheric nitrogen, which is inaccessible to plants, and releasing it in the form of ammonium ions into the soil (nitrogen fixation). In addition to being a model organism for studying diazotrophs, it is used by humans for the production of biofertilizers, food additives, and some biopolymers. The first representative of the genus, ''Azotobacter chroococcum'', was discovered and described in 1901 by Dutch microbiologist and botanist Martinus Beijerinck. ''Azotobacter'' species are Gram-negative bacteria found in neutral and alkaline soils, in water, and in association with some plants. Biological characteristics Morphology Cell (biology), Cells of the genus ...
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Azotobacter Tropicalis
''Azotobacter'' is a genus of usually motile, oval or spherical bacteria that form thick-walled cysts (and also has hard crust) and may produce large quantities of capsular slime. They are aerobic, free-living soil microbes that play an important role in the nitrogen cycle in nature, binding atmospheric nitrogen, which is inaccessible to plants, and releasing it in the form of ammonium ions into the soil (nitrogen fixation). In addition to being a model organism for studying diazotrophs, it is used by humans for the production of biofertilizers, food additives, and some biopolymers. The first representative of the genus, '' Azotobacter chroococcum'', was discovered and described in 1901 by Dutch microbiologist and botanist Martinus Beijerinck. ''Azotobacter'' species are Gram-negative bacteria found in neutral and alkaline soils, in water, and in association with some plants. Biological characteristics Morphology Cells of the genus ''Azotobacter'' are relatively large for ...
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Azotobacter Armeniacus
''Azotobacter'' is a genus of usually motile, oval or spherical bacteria that form thick-walled cysts (and also has hard crust) and may produce large quantities of capsular slime. They are aerobic, free-living soil microbes that play an important role in the nitrogen cycle in nature, binding atmospheric nitrogen, which is inaccessible to plants, and releasing it in the form of ammonium ions into the soil (nitrogen fixation). In addition to being a model organism for studying diazotrophs, it is used by humans for the production of biofertilizers, food additives, and some biopolymers. The first representative of the genus, '' Azotobacter chroococcum'', was discovered and described in 1901 by Dutch microbiologist and botanist Martinus Beijerinck. ''Azotobacter'' species are Gram-negative bacteria found in neutral and alkaline soils, in water, and in association with some plants. Biological characteristics Morphology Cells of the genus ''Azotobacter'' are relatively large for ...
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Azotobacter Salinestris
''Azotobacter salinestris'' is a Gram-negative, nitrogen-fixing bacterium; its specific name, ''salinestris'', comes from the Latin words ''salinus'' meaning saline and ''estris'' which means "living in".Page, and Shivprasad. "ITIS Standard Report Page: Azotobacter Salinestris." ITIS Standard Report Page: Azotobacter Salinestris. N.p., 1991. Web. 8 Feb. 2016. It can be found living in soil or marine habitats as single cells or in chains of six to eight cells. This organism is motile at younger stages, but loses its flagella at older stages. This species is known for its potential use in bioremediation. Isolation William J. Page and Shailaja Shivprasad isolated ''A. salinestris'' from saline soils. The colonies used for their study were first taken from air-dried surface soil from Alberta, Canada. The soil was inoculated into a Burk nitrogen-free mineral-salt medium, which contained 1% glucose and 0.25 micrograms of copper chloride per milliliter of solution. The medium was ...
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Azotobacter Chroococcum
''Azotobacter chroococcum'' is a bacterium that has the ability to fix atmospheric nitrogen. It was discovered by Martinus Beijerinck in 1901, and was the first aerobic, free-living nitrogen fixer discovered. ''A. chroococcum'' could be useful for nitrogen fixation in crops as a biofertilizer, fungicide, and nutrient indicator, and in bioremediation. Characteristics ''A. chroococcum'' is a microaerophilic plant growth-promoting rhizobacterium (PGRP), which is bacillus in shape and is Gram negative. As a mesophile, this bacterium grows best in moderate-temperature soils and requires a neutral pH environment. It is able to fix nitrogen under aerobic conditions. The soil cannot be poor in phosphorus or else nitrogen fixing can be hindered. In addition to phosphorus, these bacteria needed potassium, "sulphur, magnesium, and calcium" to grow. To fix nitrogen ''A. chroococcum'' produces three enzymes ( catalase, peroxidase, and superoxide dismutase) to "neutralise" reactive oxygen ...
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Azotobacter Vinelandii
''Azotobacter vinelandii'' is Gram-negative diazotroph that can fix nitrogen while grown aerobically. These bacteria are easily cultured and grown. ''A. vinelandii'' is a free-living N2 fixer known to produce many phytohormones and vitamins in soils. It produces fluorescent pyoverdine pigments. Nitrogenase The nitrogenase holoenzyme of ''A. vinelandii'' has been characterised by X-ray crystallography in both ADP tetrafluoroaluminate-bound and Mg ATP-bound states. The enzyme possesses molybdenum iron- sulfido cluster cofactors (FeMoco) as active sites, each bearing two pseudocubic iron-sulfido structures. Applications It is a genetically tractable system that is used to study nitrogen fixation. Genetically engineered strains can produce significantly higher amounts of ammonia. Appropriate ammonia emissions can provide crops with the ammonia they need without excess amounts that can pollute lakes and oceans. Variable ploidy ''A. vinelandii'' can contain up to 80 chr ...
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Diazotroph
Diazotrophs are bacteria and archaea that fix gaseous nitrogen in the atmosphere into a more usable form such as ammonia. A diazotroph is a microorganism that is able to grow without external sources of fixed nitrogen. Examples of organisms that do this are rhizobia and ''Frankia'' (in symbiosis) and '' Azospirillum''. All diazotrophs contain iron-molybdenum or iron-vanadium nitrogenase systems. Two of the most studied systems are those of ''Klebsiella pneumoniae'' and '' Azotobacter vinelandii''. These systems are studied because of their genetic tractability and their fast growth. Etymology The word diazotroph is derived from the words ''diazo'' ("di" = two + "azo" = nitrogen) meaning "dinitrogen (N2)" and ''troph'' meaning "pertaining to food or nourishment", in summary dinitrogen utilizing. The word ''azote'' means nitrogen in French and was named by French chemist and biologist Antoine Lavoisier, who saw it as the part of air which cannot sustain life. Types of diazotrophs ...
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Nitrogen Fixation
Nitrogen fixation is a chemical process by which molecular nitrogen (), with a strong triple covalent bond, in the air is converted into ammonia () or related nitrogenous compounds, typically in soil or aquatic systems but also in industry. Atmospheric nitrogen is molecular dinitrogen, a relatively nonreactive molecule that is metabolically useless to all but a few microorganisms. Biological nitrogen fixation or ''diazotrophy'' is an important microbials mediated process that converts dinitrogen (N2) gas to ammonia (NH3) using the nitrogenase protein complex (Nif). Nitrogen fixation is essential to life because fixed inorganic nitrogen compounds are required for the biosynthesis of all nitrogen-containing organic compounds, such as amino acids and proteins, nucleoside triphosphates and nucleic acids. As part of the nitrogen cycle, it is essential for agriculture and the manufacture of fertilizer. It is also, indirectly, relevant to the manufacture of all nitrogen chemical ...
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Martinus Beijerinck
Martinus Willem Beijerinck (, 16 March 1851 – 1 January 1931) was a Dutch microbiologist and botanist who was one of the founders of virology and environmental microbiology. He is credited with the discovery of viruses, which he called "''contagium vivum fluidum''". Life Early life and education Born in Amsterdam, Beijerinck studied at the Technical School of Delft, where he was awarded the degree of Chemical Engineer in 1872. He obtained his Doctor of Science degree from the University of Leiden in 1877. At the time, Delft, then a Polytechnic, did not have the right to confer doctorates, so Leiden did this for them. He became a teacher in microbiology at the Agricultural School in Wageningen (now Wageningen University) and later at the ''Polytechnische Hogeschool Delft'' (Delft Polytechnic, currently Delft University of Technology) (from 1895). He established the Delft School of Microbiology. His studies of agricultural and industrial microbiology yielded fundamental ...
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Biofertilizer
A biofertilizer is a substance which contains living micro-organisms which, when applied to seeds, plant surfaces, or soil, colonize the rhizosphere or the interior of the plant and promotes growth by increasing the supply or availability of primary nutrients to the host plant. Biofertilizers add nutrients through the natural processes of nitrogen fixation, solubilizing phosphorus, and stimulating plant growth through the synthesis of growth-promoting substances. The micro-organisms in biofertilizers restore the soil's natural nutrient cycle and build soil organic matter. Through the use of biofertilizers, healthy plants can be grown, while enhancing the sustainability and the health of the soil. Biofertilizers can be expected to reduce the use of synthetic fertilizers and pesticides, but they are not yet able to replace their use. Since they play several roles, a preferred scientific term for such beneficial bacteria is "plant-growth promoting rhizobacteria" (PGPR). Biofert ...
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