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Amphidinium Operculatum
''Amphidinium'' is a genus of dinoflagellates. The type for the genus is '' Amphidinium operculatum'' Claparède & Lachmann. The genus includes the species '' Amphidinium carterae'' which is used as a model organism. As dinoflagellates, ''Amphidinium'' spp. have chloroplasts. The ''Amphidinium'' chloroplast genome is unusual in not having a single contiguous circular genome. Instead, chloroplast genes encoded in chloroplast DNA are found on numerous 2-3 kbp minicircles, analogous to plasmids. Most minicircles have only a few protein-coding genes; many have just a single gene. There are reports of minicircles that do not have known transcripts, like in the ''Amphidinium carterae'' chloroplast genome, although these were not found in the ''Symbiodinium'' sp. Clade C3 chloroplast genome. Minicircle-derived transcripts can be processed in ways not typical of eukaryotes, including the addition of a 3' poly(U) tail, as opposed to the typical poly(A) tail derived from polyadenylati ...
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Amphidinium Sulcatum
''Amphidinium'' is a genus of dinoflagellates. The type for the genus is '' Amphidinium operculatum'' Claparède & Lachmann. The genus includes the species '' Amphidinium carterae'' which is used as a model organism. As dinoflagellates, ''Amphidinium'' spp. have chloroplasts. The ''Amphidinium'' chloroplast genome is unusual in not having a single contiguous circular genome. Instead, chloroplast genes encoded in chloroplast DNA are found on numerous 2-3 kbp minicircles, analogous to plasmids. Most minicircles have only a few protein-coding genes; many have just a single gene. There are reports of minicircles that do not have known transcripts, like in the ''Amphidinium carterae'' chloroplast genome, although these were not found in the ''Symbiodinium'' sp. Clade C3 chloroplast genome. Minicircle-derived transcripts can be processed in ways not typical of eukaryotes, including the addition of a 3' poly(U) tail, as opposed to the typical poly(A) tail derived from polyadenylati ...
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Dinoflagellate
The dinoflagellates (Greek δῖνος ''dinos'' "whirling" and Latin ''flagellum'' "whip, scourge") are a monophyletic group of single-celled eukaryotes constituting the phylum Dinoflagellata and are usually considered algae. Dinoflagellates are mostly marine plankton, but they also are common in freshwater habitats. Their populations vary with sea surface temperature, salinity, and depth. Many dinoflagellates are photosynthetic, but a large fraction of these are in fact mixotrophic, combining photosynthesis with ingestion of prey (phagotrophy and myzocytosis). In terms of number of species, dinoflagellates are one of the largest groups of marine eukaryotes, although substantially smaller than diatoms. Some species are endosymbionts of marine animals and play an important part in the biology of coral reefs. Other dinoflagellates are unpigmented predators on other protozoa, and a few forms are parasitic (for example, ''Oodinium'' and ''Pfiesteria''). Some dinoflagellates pro ...
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Journal Of Organic Chemistry
''The Journal of Organic Chemistry'', colloquially known as ''JOC'', is a peer-reviewed scientific journal for original contributions of fundamental research in all branches of theory and practice in organic and bioorganic chemistry. It is published by the publishing arm of the American Chemical Society, with 24 issues per year. According to the ''Journal Citation Reports'', the journal had a 2017 impact factor of 4.805 and it is the journal that received the most cites (100,091 in 2017) in the field of organic chemistry. According to Web of Knowledge (and as December 2012), eleven papers from the journal have received more than 1,000 citations, with the most cited paper having received 7,967 citations. The current editor-in-chief is Scott J. Miller from Yale University. Indexing ''J. Org. Chem.'' is currently indexed in: See also *Organic Letters *Organometallics ''Organometallics'' is a biweekly journal published by the American Chemical Society. Its area of focus is ...
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Luteophanol
Luteophanol is a type of organic compound. There are variants labeled luteophanol A through luteophanol D. They contain the following common fragments with amphidinols: # Polyhydroxyl groups. # Two tetrahydropyran rings. Luteophanols have demonstrated antibacterial properties, but unlike amphidinols, they do not show antifungal activity. :{{clear External links Y. Doi et al., "Luteophanol A, a New Polyhydroxyl Compound from Symbiotic Marine Dinoflagellate Amphidinium sp." Journal of Organic Chemistry ''The Journal of Organic Chemistry'', colloquially known as ''JOC'', is a peer-reviewed scientific journal for original contributions of fundamental research in all branches of theory and practice in organic and bioorganic chemistry. It is publi ... (1997) (abstract)Abstract of article on Luteophanol D Ethers Alkene derivatives Tetrahydropyrans Polyols ...
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Polyadenylation
Polyadenylation is the addition of a poly(A) tail to an RNA transcript, typically a messenger RNA (mRNA). The poly(A) tail consists of multiple adenosine monophosphates; in other words, it is a stretch of RNA that has only adenine bases. In eukaryotes, polyadenylation is part of the process that produces mature mRNA for translation. In many bacteria, the poly(A) tail promotes degradation of the mRNA. It, therefore, forms part of the larger process of gene expression. The process of polyadenylation begins as the transcription of a gene terminates. The 3′-most segment of the newly made pre-mRNA is first cleaved off by a set of proteins; these proteins then synthesize the poly(A) tail at the RNA's 3′ end. In some genes these proteins add a poly(A) tail at one of several possible sites. Therefore, polyadenylation can produce more than one transcript from a single gene (alternative polyadenylation), similar to alternative splicing. The poly(A) tail is important for the nuclea ...
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Gene
In biology, the word gene (from , ; "...Wilhelm Johannsen coined the word gene to describe the Mendelian units of heredity..." meaning ''generation'' or ''birth'' or ''gender'') can have several different meanings. The Mendelian gene is a basic unit of heredity and the molecular gene is a sequence of nucleotides in DNA that is transcribed to produce a functional RNA. There are two types of molecular genes: protein-coding genes and noncoding genes. During gene expression, the DNA is first copied into RNA. The RNA can be directly functional or be the intermediate template for a protein that performs a function. The transmission of genes to an organism's offspring is the basis of the inheritance of phenotypic traits. These genes make up different DNA sequences called genotypes. Genotypes along with environmental and developmental factors determine what the phenotypes will be. Most biological traits are under the influence of polygenes (many different genes) as well as gen ...
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Plasmids
A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently. They are most commonly found as small circular, double-stranded DNA molecules in bacteria; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that benefit the survival of the organism and confer selective advantage such as antibiotic resistance. While chromosomes are large and contain all the essential genetic information for living under normal conditions, plasmids are usually very small and contain only additional genes that may be useful in certain situations or conditions. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms. In the laboratory, plasmids may be introduced into a cell via transformation. Synthetic plasmids are available for procurement over the intern ...
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Minicircles
Minicircles are small (~4 kb) circular replicons. They occur naturally in some eukaryotic organelle genomes. In the mitochondria-derived kinetoplast of trypanosomes, minicircles encode guide RNAs for RNA editing. In ''Amphidinium'', the chloroplast genome is made of minicircles that encode chloroplast proteins. ''In vitro'' experimentally-derived minicircles Minicircles are small (~4kb) circular plasmid derivatives that have been freed from all prokaryotic vector parts. They have been applied as transgene carriers for the genetic modification of mammalian cells, with the advantage that, since they contain no bacterial DNA sequences, they are less likely to be perceived as foreign and destroyed. (Typical transgene delivery methods involve plasmids, which contain foreign DNA.) The smaller size of minicircles also extends their cloning capacity and facilitates their delivery into cells. Their preparation usually follows a two-step procedure: * production of a 'parental plas ...
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Genome
In the fields of molecular biology and genetics, a genome is all the genetic information of an organism. It consists of nucleotide sequences of DNA (or RNA in RNA viruses). The nuclear genome includes protein-coding genes and non-coding genes, other functional regions of the genome such as regulatory sequences (see non-coding DNA), and often a substantial fraction of 'junk' DNA with no evident function. Almost all eukaryotes have mitochondria and a small mitochondrial genome. Algae and plants also contain chloroplasts with a chloroplast genome. The study of the genome is called genomics. The genomes of many organisms have been sequenced and various regions have been annotated. The International Human Genome Project reported the sequence of the genome for ''Homo sapiens'' in 200The Human Genome Project although the initial "finished" sequence was missing 8% of the genome consisting mostly of repetitive sequences. With advancements in technology that could handle sequenci ...
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Chloroplasts
A chloroplast () is a type of membrane-bound organelle known as a plastid that conducts photosynthesis mostly in plant cell, plant and algae, algal cells. The photosynthetic pigment chlorophyll captures the energy from sunlight, converts it, and stores it in the energy-storage molecules Adenosine triphosphate, ATP and NADPH while freeing oxygen from water in the cells. The ATP and NADPH is then used to make organic molecules from carbon dioxide in a process known as the Calvin cycle. Chloroplasts carry out a number of other functions, including fatty acid synthesis, amino acid synthesis, and the immune response in plants. The number of chloroplasts per cell varies from one, in unicellular algae, up to 100 in plants like ''Arabidopsis'' and wheat. A chloroplast is characterized by Chloroplast membrane, its two membranes and a high concentration of chlorophyll. Other plastid types, such as the leucoplast and the chromoplast, contain little chlorophyll and do not carry out photos ...
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Model Organism
A model organism (often shortened to model) is a non-human species that is extensively studied to understand particular biological phenomena, with the expectation that discoveries made in the model organism will provide insight into the workings of other organisms. Model organisms are widely used to research human disease when human experimentation would be unfeasible or unethical. This strategy is made possible by the common descent of all living organisms, and the conservation of metabolic and developmental pathways and genetic material over the course of evolution. Studying model organisms can be informative, but care must be taken when generalizing from one organism to another. In researching human disease, model organisms allow for better understanding the disease process without the added risk of harming an actual human. The species chosen will usually meet a determined taxonomic equivalency to humans, so as to react to disease or its treatment in a way that resembles ...
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