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AH109
AH109 is a strain of yeast (''Saccharomyces cerevisiae'') used in biological research for two-hybrid screening. The strain is sold commercially by Clontech and is used as a partner with strain Y187 in mating In biology, mating is the pairing of either opposite-sex or hermaphroditic organisms for the purposes of sexual reproduction. ''Fertilization'' is the fusion of two gametes. ''Copulation'' is the union of the sex organs of two sexually reproduc ... assays. References Yeasts {{yeast-stub ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Two-hybrid Screening
Two-hybrid screening (originally known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively. The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the DNA-binding domain (DBD or often also abbreviated as BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay. History Pioneered by Stanley Fields and Ok-Kyu Song in 1989, the technique was originally designed to detect protein–protein ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Y187
Y187 is a strain of yeast (''Saccharomyces cerevisiae'') used in biological research for two-hybrid screening. The strain has been sold commercially by Clontech since at least 2000 and is used as a partner with strain AH109 in mating In biology, mating is the pairing of either opposite-sex or hermaphroditic organisms for the purposes of sexual reproduction. ''Fertilization'' is the fusion of two gametes. ''Copulation'' is the union of the sex organs of two sexually reproduc ... assays. Genotype of Y187: MATα, ura3-52, his3-200, ade2-101, trp1-901, leu2-3, 112, gal4Δ, met–, gal80Δ, URA3::GAL1UAS-GAL1TATA-lacZ.http://www.uni-jena.de/unijenamedia/Downloads/faculties/bio_pharm/ls_genetik/Teaching/Grundpraktikum/Y2Hscript_SS11.pdf References Yeasts {{yeast-stub ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Saccharomyces Cerevisiae
''Saccharomyces cerevisiae'' () (brewer's yeast or baker's yeast) is a species of yeast (single-celled fungus microorganisms). The species has been instrumental in winemaking, baking, and brewing since ancient times. It is believed to have been originally isolated from the skin of grapes. It is one of the most intensively studied eukaryotic model organisms in molecular biology, molecular and cell biology, much like ''Escherichia coli'' as the model bacteria, bacterium. It is the microorganism behind the most common type of fermentation (biochemistry), fermentation. ''S. cerevisiae'' cells are round to ovoid, 5–10 micrometre, μm in diameter. It reproduces by budding. Many proteins important in human biology were first discovered by studying their Homology (biology), homologs in yeast; these proteins include cell cycle proteins, signaling proteins, and protein-processing enzymes. ''S. cerevisiae'' is currently the only yeast cell known to have Berkeley body, Berkeley bo ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Takara Holdings
is a Japanese company based in Kyoto. The company is mainly involved in the production of beverages, food, printing and medical supplies. Divisions Takara Bio Clontech Laboratories was acquired from BD Biosciences in 2005. In 2007, Clontech was involved in litigation with Invitrogen over patents for RNase H minus reverse transcriptase. Subsidiaries of Takara Bio Inc. include Takara Bio USA (formerly Clontech Laboratories), a Mountain View, California-based manufacturer of kits, reagents, instruments, and services for biological research, as well as regional subsidiaries in Europe, Korea, China, and India. The main offices of Takara Bio USA moved to San Jose in August 2021. Takara Shuzo Takara Shuzo Co. produces sake Sake, also spelled saké ( ; also referred to as Japanese rice wine), is an alcoholic beverage of Japanese origin made by fermenting rice that has been polished to remove the bran. Despite the name ''Japanese rice wine'', sake, and indee ..., other bev ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mating Of Yeast
The yeast ''Saccharomyces cerevisiae'' is a simple microorganism, single-celled eukaryote with both a diploid and haploid mode of existence. The mating of yeast only occurs between haploids, which can be either the a or α (alpha) mating type and thus display simple sexual differentiation. Mating type is determined by a single locus (genetics), locus, ''MAT'', which in turn governs the sexual behaviour of both haploid and diploid cells. Through a form of genetic recombination, haploid yeast can switch mating type as often as every cell cycle. Mating type and the life cycle of ''Saccharomyces cerevisiae'' ''S. cerevisiae'' (yeast) can stably exist as either a diploid or a haploid. Both haploid and diploid yeast cells reproduce by mitosis, with daughter cells budding off of mother cells. Haploid cells are capable of mating with other haploid cells of the opposite mating type (an a cell can only mate with an α cell, and vice versa) to produce a stable diploid cell. Diploid cells, usu ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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