Y187 is a strain of yeast (''
Saccharomyces cerevisiae
''Saccharomyces cerevisiae'' () (brewer's yeast or baker's yeast) is a species of yeast (single-celled fungus microorganisms). The species has been instrumental in winemaking, baking, and brewing since ancient times. It is believed to have been o ...
'') used in biological research for
two-hybrid screening
Two-hybrid screening (originally known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as bindi ...
. The strain has been sold commercially by
Clontech since at least 2000 and is used as a partner with strain
AH109 AH109 is a strain of yeast (''Saccharomyces cerevisiae'') used in biological research for two-hybrid screening. The strain is sold commercially by Clontech and is used as a partner with strain Y187 in mating
In biology, mating is the pairing ...
in
mating
In biology, mating is the pairing of either opposite-sex or hermaphroditic organisms for the purposes of sexual reproduction. ''Fertilization'' is the fusion of two gametes. ''Copulation'' is the union of the sex organs of two sexually reproduc ...
assays.
Genotype of Y187: MATα, ura3-52, his3-200, ade2-101, trp1-901, leu2-3, 112, gal4Δ, met–, gal80Δ, URA3::GAL1
UAS-GAL1
TATA-lacZ.
[http://www.uni-jena.de/unijenamedia/Downloads/faculties/bio_pharm/ls_genetik/Teaching/Grundpraktikum/Y2Hscript_SS11.pdf]
References
Yeasts
{{yeast-stub