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Acholeplasma Laidlawii
''Acholeplasma laidlawii'' are small bacteria which lack a cell wall. Like other Acholeplasma and Mycoplasma, ''A. laidlawii'' has been identified as a common contaminant of growth media for cell culture. History ''A. laidlawii'' was first isolated from sewage in London in 1936 and was named after its discoverer, Patrick Laidlaw. Genetics ''A. laidlawii'' has a relatively small genome comprising 1.5 Mbp. Additionally its genome has a low GC-content of just 31%. The ''A. laidlawii'' genome has been sequenced. In Research ''Acholeplasma laidlawii'' may contaminate bovine serum and also occurs in serum-free cell culture media products. The presence of ''A. laidlawii'' in broth powders is a serious problem in routine biopharmaceutical operations where filtration is used as a sterilisation procedure. ''A. laidlawii'' may flourish and survive for prolonged periods at refrigeration and ambient temperatures in serum-free cell culture media. References External links Type str ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
LPSN
List of Prokaryotic names with Standing in Nomenclature (LPSN) is an online database that maintains information on the naming and taxonomy of prokaryotes, following the taxonomy requirements and rulings of the International Code of Nomenclature of Prokaryotes The International Code of Nomenclature of Prokaryotes (ICNP) formerly the International Code of Nomenclature of Bacteria (ICNB) or Bacteriological Code (BC) governs the scientific names for Bacteria and Archaea.P. H. A. Sneath, 2003. A short hist .... The database was curated from 1997 to June 2013 by Jean P. Euzéby. From July 2013 to January 2020, LPSN was curated by Aidan C. Parte. In February 2020, a new version of LPSN was published as a service of the Leibniz Institute DSMZ, thereby also integrating the Prokaryotic Nomenclature Up-to-date service. References External links List of Prokaryotic names with Standing in Nomenclature [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Acholeplasma
''Acholeplasma'' are wall-less bacteria in the Mollicutes class. They include saprotrophic or pathogenic species. There are 15 recognised species. The G+C content is low, ranging from 26 - 36% ( mol%). The genomes of ''Acholeplasma'' species range in size from 1.5 to 1.65 Mbp. Cholesterol is not required for growth. The species are found on animals, and some plants and insects. The optimum growth temperature is 30 to 37 degrees Celsius. ''Acholeplasma laidlawii'' is a common contaminant of cell culture media products, and has also been used in extensive studies of lipid polymorphism because this organism alters its ratio of MGlcDG (monoglucosyl diacylglycerol) to DGlcDG (diglucosyl diacylglycerol) in response to growth conditions. Phylogeny See also * List of bacteria genera * List of bacterial orders This article lists the orders of the Bacteria. The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature (LPSN) and National Center ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mycoplasma
''Mycoplasma'' is a genus of bacteria that, like the other members of the class ''Mollicutes'', lack a cell wall around their cell membranes. Peptidoglycan (murein) is absent. This characteristic makes them naturally resistant to antibiotics that target cell wall synthesis (like the beta-lactam antibiotics). They can be parasitic or saprotrophic. Several species are pathogenic in humans, including '' M. pneumoniae'', which is an important cause of "walking" pneumonia and other respiratory disorders, and '' M. genitalium'', which is believed to be involved in pelvic inflammatory diseases. ''Mycoplasma'' species (like the other species of the class ''Mollicutes'') are among the smallest organisms yet discovered, can survive without oxygen, and come in various shapes. For example, ''M. genitalium'' is flask-shaped (about 300 x 600 nm), while ''M. pneumoniae'' is more elongated (about 100 x 1000 nm), many ''Mycoplasma'' species are coccoid. Hundreds of ''Mycoplasma'' species i ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Growth Media
A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation or small plants like the moss ''Physcomitrella patens''. Different types of media are used for growing different types of cells. The two major types of growth media are those used for cell culture, which use specific cell types derived from plants or animals, and those used for microbiological culture, which are used for growing microorganisms such as bacteria or fungi. The most common growth media for microorganisms are nutrient broths and agar plates; specialized media are sometimes required for microorganism and cell culture growth. Some organisms, termed fastidious organisms, require specialized environments due to complex nutritional requirements. Viruses, for example, are obligate intracellular parasites and require a growth medium containing living cells. Types The most common growth media fo ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Patrick Laidlaw
Sir Patrick Playfair Laidlaw (26 September 1881 – 20 March 1940) was a Scottish virologist. Biography Laidlaw was born in Glasgow, the son of Robert Laidlaw, M.D., at that time Superintendent of the Glasgow Medical Mission. He was educated at Leys School, Cambridge and St. John’s College, Cambridge. From 1920–23, he studied the properties of histamine at the Wellcome Physiological Research Laboratories after which he went to Guy's Hospital as a lecturer in experimental pathology. As a virologist at the Medical Research Council in 1922 his researches on dog-distemper led to two ways of immunisation against it, which achievement earned him the award of a Royal Medal by the Royal Society in 1933. In 1927 he had been elected a fellow of the Royal Society. He was one of the scientists working at the Medical Research Council ( NIMR Farm Laboratories) at Mill Hill who first isolated influenza virus from humans. This happened when ferrets they were working on to develop a di ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Base Pair
A base pair (bp) is a fundamental unit of double-stranded nucleic acids consisting of two nucleobases bound to each other by hydrogen bonds. They form the building blocks of the DNA double helix and contribute to the folded structure of both DNA and RNA. Dictated by specific hydrogen bonding patterns, "Watson–Crick" (or "Watson–Crick–Franklin") base pairs (guanine–cytosine and adenine–thymine) allow the DNA helix to maintain a regular helical structure that is subtly dependent on its nucleotide sequence. The Complementarity (molecular biology), complementary nature of this based-paired structure provides a redundant copy of the genetic information encoded within each strand of DNA. The regular structure and data redundancy provided by the DNA double helix make DNA well suited to the storage of genetic information, while base-pairing between DNA and incoming nucleotides provides the mechanism through which DNA polymerase replicates DNA and RNA polymerase transcribes DNA in ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
GC-content
In molecular biology and genetics, GC-content (or guanine-cytosine content) is the percentage of nitrogenous bases in a DNA or RNA molecule that are either guanine (G) or cytosine (C). This measure indicates the proportion of G and C bases out of an implied four total bases, also including adenine and thymine in DNA and adenine and uracil in RNA. GC-content may be given for a certain fragment of DNA or RNA or for an entire genome. When it refers to a fragment, it may denote the GC-content of an individual gene or section of a gene (domain), a group of genes or gene clusters, a non-coding region, or a synthetic oligonucleotide such as a primer. Structure Qualitatively, guanine (G) and cytosine (C) undergo a specific hydrogen bonding with each other, whereas adenine (A) bonds specifically with thymine (T) in DNA and with uracil (U) in RNA. Quantitatively, each GC base pair is held together by three hydrogen bonds, while AT and AU base pairs are held together by two hydrogen bonds. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
National Center For Biotechnology Information
The National Center for Biotechnology Information (NCBI) is part of the United States National Library of Medicine (NLM), a branch of the National Institutes of Health (NIH). It is approved and funded by the government of the United States. The NCBI is located in Bethesda, Maryland, and was founded in 1988 through legislation sponsored by US Congressman Claude Pepper. The NCBI houses a series of databases relevant to biotechnology and biomedicine and is an important resource for bioinformatics tools and services. Major databases include GenBank for DNA sequences and PubMed, a bibliographic database for biomedical literature. Other databases include the NCBI Epigenomics database. All these databases are available online through the Entrez search engine. NCBI was directed by David Lipman, one of the original authors of the BLAST sequence alignment program and a widely respected figure in bioinformatics. GenBank NCBI had responsibility for making available the GenBank DNA seque ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mollicutes
Mollicutes is a class of bacteria distinguished by the absence of a cell wall. The word "Mollicutes" is derived from the Latin ''mollis'' (meaning "soft" or "pliable"), and ''cutis'' (meaning "skin"). Individuals are very small, typically only 0.2–0.3 μm (200-300 nm) in size and have a very small genome size. They vary in form, although most have sterols that make the cell membrane somewhat more rigid. Many are able to move about through gliding, but members of the genus ''Spiroplasma'' are helical and move by twisting. The best-known genus in the Mollicutes is ''Mycoplasma''. Colonies show the typical "fried-egg" appearance. Mollicutes are parasites of various animals and plants, living on or in the host's cells. Many cause diseases in humans, attaching to cells in the respiratory or urogenital tracts, particularly species of ''Mycoplasma'' and ''Ureaplasma''. Phytoplasma and ''Spiroplasma'' are plant pathogens associated with insect vectors. Whereas formerly the trivial n ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
