Tennis at the 2000 Summer Olympics – Women's singles

The United States' Venus Williams defeated Russia's Elena Dementieva in the final, 6–2, 6–4 to win the gold medal in Women's Singles tennis at the 2000 Summer Olympics. In the bronze medal match, the United States' Monica Seles defeated Australia's Jelena Dokic, 6–1, 6–4. Williams' victory was the third consecutive gold medal in the women's singles for the United States, all by different players (preceded by Jennifer Capriati and Lindsay Davenport).

The tournament was held at the Sydney Olympic Park Tennis Centre in Sydney, Australia from 19 September until 28 September. There were 64 competitors from 33 nations, with each nation having up to 3 players.

The United States' Davenport was the defending gold medalist from 1996, but she withdrew from her second round match due to injury.