Phosphoribosylformylglycinamidine synthase

In enzymology, a phosphoribosylformylglycinamidine synthase (EC6.3.5.3) is an enzyme that catalyzes the chemical reaction

ATP + N₂-formyl-N₁-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H₂O ${\displaystyle ightleftharpoons }$ ADP + phosphate + 2-(formamido)-N₁-(5-phospho-D-ribosyl)acetamidine + L-glutamate

The 4 substrates of this enzyme are ATP, , L-glutamine, and H₂O, whereas its 4 products are ADP, phosphate, , and L-glutamate.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds carbon-nitrogen ligases with glutamine as amido-N-donor. The systematic name of this enzyme class is N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide:L-glutamine amido-ligase (ADP-forming). Other names in common use include phosphoribosylformylglycinamidine synthetase, formylglycinamide ribonucloetide amidotransferase, phosphoribosylformylglycineamidine synthetase, FGAM synthetase, FGAR amidotransferase, 5'-phosphoribosylformylglycinamide:L-glutamine amido-ligase, (ADP-forming), 2-N-formyl-1-N-(5-phospho-D-ribosyl)glycinamide:L-glutamine, and amido-ligase (ADP-forming).

It is known as ADE6 in Saccharomyces cerevisiae (budding yeast) genetics.