Malate synthase

Malate synthase (EC2.3.3.9) is an enzyme that catalyzes the chemical reaction

glyoxylic acid

+

acetyl-CoA

H₂O

(S-malic acid

+ coenzyme A

The three substrates of this enzyme are glyoxylic acid, acetyl-CoA, and water. Its products are the (S) enantiomer of malic acid, and coenzyme A. This enzyme participates in pyruvate metabolism and glyoxylate and dicarboxylate metabolism.