Topic summary
Dark-field illumination
Extracted from the Wikipedia article Dark-field microscopy.
Advantages and disadvantages
- Comparison of transillumination techniques used to generate contrast in a sample of tissue paper (1.559 μm/pixel when viewed at full resolution)
- Dark-field illumination, sample contrast comes from light scattered by the sample
- Bright-field illumination, sample contrast comes from attenuation of light in the sample
- Cross-polarized light illumination, sample contrast comes from rotation of polarized light through the sample
- Phase-contrast illumination, sample contrast comes from interference of different path lengths of light through the sample