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Flow cytometry

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Flow cytometry (FC) is a technique used to detect and measure the physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components.

FluorescenceFluorescenceFluorescence is one of two kinds of photoluminescence, the emission of light by a substance that has absorbed light or other electromagnetic radiation. When exposed to ultraviolet radiation, many substances will glow (fluoresce) with colored visible light. The color of the light emitted depends on the chemical composition of the substance. Fluorescent materials generally cease to glow nearly immediately when the radiation source stops.Absorption (electromagnetic radiation)Absorption (electromagnetic radiation)In physics, absorption of electromagnetic radiation is how matter (typically electrons bound in atoms) takes up a photon's energy—and so transforms electromagnetic energy into internal energy of the absorber (for example, thermal energy). A notable effect of the absorption of electromagnetic radiation is attenuation of the radiation; attenuation is the gradual reduction of the intensity of light waves as they propagate through a medium.Wallace H. CoulterWallace H. CoulterWallace H. Coulter (February 17, 1913 – August 7, 1998) was an American electrical engineer, inventor, and businessman. The best known of his 85 patents is the Coulter principle, which provides a method for counting and sizing microscopic particles suspended in fluid. His invention of the Coulter Counter made possible today's most common medical diagnostic test: the complete blood count (CBC).CytometryCytometryCytometry is the measurement of number and characteristics of cells. Variables that can be measured by cytometric methods include cell size, cell count, cell morphology (shape and structure), cell cycle phase, DNA content, and the existence or absence of specific proteins on the cell surface or in the cytoplasm. Cytometry is used to characterize and count blood cells in common blood tests such as the complete blood count.Electrical impedanceElectrical impedanceIn electrical engineering, impedance is the opposition to alternating current presented by the combined effect of resistance and reactance in a circuit. Quantitatively, the impedance of a two-terminal circuit element is the ratio of the complex representation of the sinusoidal voltage between its terminals, to the complex representation of the current flowing through it. In general, it depends upon the frequency of the sinusoidal voltage.Coulter counterCoulter counterA Coulter counter is an apparatus for counting and sizing particles suspended in electrolytes. The Coulter counter is the commercial term for the technique known as resistive pulse sensing or electrical zone sensing. The apparatus is based on the Coulter principle named after its inventor, Wallace H. Coulter. A typical Coulter counter has one or more microchannels that separate two chambers containing electrolyte solutions.GöttingenGöttingenGöttingen (, US also ; German:; Low German: Chöttingen) is a university city in Lower Saxony, central Germany, the capital of the eponymous district. The River Leine runs through it. According to the 2022 German census, the population of Göttingen was 124,548. OverviewThe origins of Göttingen lay in a village called Gutingi, first mentioned in a document in 953 AD. The city was founded northwest of this village, between 1150 and 1200 AD, and adopted its name.Science (journal)Science (journal)Science is the peer-reviewedacademic journal of the American Association for the Advancement of Science (AAAS) and one of the world's top academic journals. It was first published in 1880, is currently circulated weekly and has a subscriber base of around 130,000. Because institutional subscriptions and online access serve a larger audience, its estimated readership is over 400,000 people.University of MünsterUniversity of MünsterThe University of Münster (German: Universität Münster, until 2023 German: Westfälische Wilhelms-Universität Münster, WWU) is a public research university located in the city of Münster, North Rhine-Westphalia in Germany. With more than 43,000 students and over 120 fields of study in 15 departments, it is Germany's fifth largest university and one of the foremost centers of German intellectual life.Cell (biology)Cell (biology)Basic unit of life formsThe cell is the basic structural and functional unit of all forms of life or organisms. The term comes from the Latin word cellula meaning 'small room'. A biological cell basically consists of a semipermeablecell membrane enclosing cytoplasm that contains genetic material. Most cells are only visible under a microscope.Clinical trialClinical trialPhase of clinical research in medicineA clinical trial participant receives an injection.Clinical trials are prospective biomedical or behavioral research studies on human participants designed to answer specific questions about biomedical or behavioral interventions, including new treatments (such as novel vaccines, drugs, dietary choices, dietary supplements, and medical devices) and known interventions that warrant further study and comparison.BD (company)BD (company)Becton, Dickinson and Company (BD; also Becton Dickinson or Becton) is an American multinational medical technology company that manufactures and sells medical devices, instrument systems, and reagents. BD also provides consulting and analytics services in certain areas. BD is ranked #211 in the 2024 Fortune 500 list based on its revenues for the fiscal year ending September30, 2023.

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