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Dual-polarization interferometry

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Dual-polarization interferometry (DPI) is an analytical technique that probes molecular layers adsorbed to the surface of a waveguide using the evanescent wave of a laser beam. It is used to measure the conformational change in proteins, or other biomolecules, as they function (referred to as the conformation activity relationship). InstrumentationDPI focuses laser light into two waveguides.

Polarization (waves)Polarization (waves)Polarization, or polarisation, is a property of transverse waves which specifies the geometrical orientation of the oscillations. In a transverse wave, the direction of the oscillation is perpendicular to the direction of motion of the wave. One example of a polarized transverse wave is vibrations traveling along a taut string, for example, in a musical instrument like a guitar string.Refractive indexRefractive indexProperty in opticsA ray of light being refracted through a glass slabRefraction of a light rayIn optics, the refractive index (also called refraction index or index of refraction), often denoted n, is the ratio of the speed of light in vacuum (c) to the speed of light in a given optical medium (v), n=c/v. The refractive index determines how much the path of light is bent, or refracted, when entering a material, as described by Snell's law of refraction, n1 sin θ1 = n2 sin θ2, where θ1 and θ2 are the angle of incidence and angle of refraction, respectively, of a ray crossing the interface between two media with refractive indices n1 and n2.Waveguide (optics)Waveguide (optics)An optical waveguide is a physical structure that guides electromagnetic waves in the optical spectrum. Common types of optical waveguides include optical fiber waveguides, transparent dielectric waveguides made of plastic and glass, liquid light guides, and liquid waveguides. Optical waveguides are used as components in integrated optical circuits or as the transmission medium in local and long-haul optical communication systems.Conformational changeIn biochemistry, a conformational change is a change in the shape of a macromolecule, often induced by environmental factors. A macromolecule is usually flexible and dynamic. It can change its shape in response to changes in its environment or other factors; each possible shape is called a conformation, and a transition between them is called a conformational change.Chemical reactionChemical reactionProcess that leads to chemical changesA thermite reaction using iron(III) oxide. The sparks flying outwards are globules of molten iron trailing smoke in their wake.A chemical reaction is a process that leads to the chemical transformation of one set of chemical substances to another. When chemical reactions occur, the atoms are rearranged and the reaction is accompanied by an energy change as new products are generated.LaserLaserA laser is a device that emits light through a process of optical amplification based on the stimulated emission of electromagnetic radiation. The word laser originated as an acronym for light amplification by stimulated emission of radiation. The first laser was built in 1960 by Theodore Maiman at Hughes Research Laboratories, based on theoretical work by Charles H. Townes and Arthur Leonard Schawlow and the optical amplifier patented by Gordon Gould.Protein–lipid interactionProtein–lipid interactionProtein–lipid interaction is the influence of membrane proteins on the lipidphysical state or vice versa. The questions which are relevant to understanding of the structure and function of the membrane are: 1) Do intrinsic membrane proteins bind tightly to lipids (see annular lipid shell), and what is the nature of the layer of lipids adjacent to the protein? 2) Do membrane proteins have long-range effects on the order or dynamics of membrane lipids?Conformation–activity relationshipIn biochemistry, the conformation–activity relationship is the relationship between the biological activity and the chemical structure or conformational changes of a biomolecule. This terminology emphasizes the importance of dynamic conformational changes for the biological function, rather than the importance of static three-dimensional structure used in the analysis of structure–activity relationships.Bio-layer interferometryBio-layer interferometryBio-layer interferometry (BLI) is an optical biosensing technology that analyzes biomolecular interactions in real-time without the need for fluorescent labeling. Alongside surface plasmon resonance (SPR), BLI is one of few widely available label-free biosensing technologies, a detection style that yields more information in less time than traditional processes.BiochemistryBiochemistryStudy of chemical processes of living organismsBiochemistry, or biological chemistry (distinct from chemical biology), is the study of chemical processes within and relating to living organisms. A sub-discipline of both chemistry and biology, biochemistry may be divided into three fields: structural biology, enzymology, and metabolism. Over the last decades of the 20th century, biochemistry has become successful at explaining living processes through these three disciplines.Wave interferenceWave interferenceIn physics, interference is a phenomenon in which two coherentwaves are combined by adding their intensities or displacements with due consideration for their phase difference. The resultant wave may have greater amplitude (constructive interference) or lower amplitude (destructive interference) if the two waves are in phase or out of phase, respectively.Evanescent fieldEvanescent fieldIn electromagnetics, an evanescent field, or evanescent wave, is an oscillating electric and/or magnetic field that does not propagate as an electromagnetic wave but whose energy is spatially concentrated in the vicinity of the source (oscillating charges and currents).InteractomeInteractomeIn molecular biology, an interactome is the whole set of molecular interactions in a particular cell. The term specifically refers to physical interactions among molecules (such as those among proteins, also known as protein–protein interactions (PPIs); or between small molecules and proteins.) but can also describe sets of indirect interactions among genes (genetic interactions).

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