Reversed-phase chromatographyReversed-phase liquid chromatography (RP-LC) is a mode of liquid chromatography in which non-polar stationary phase and polar mobile phases are used for the separation of organic compounds. In the reversed phase mode, the more hydrophobic sample components are retained in the system for longer. The vast majority of separations and analyses using high-performance liquid chromatography (HPLC) in recent years are done using the reversed phase mode.
ChromatographySet of laboratory techniques for separation of mixtures Thin-layer chromatography is used to separate components of a plant extract, illustrating the experiment with plant pigments which gave chromatography its name In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components.
Thin-layer chromatographyThin-layer chromatography (TLC) is a chromatography technique that separates components in non-volatile mixtures. It is performed on a TLC plate made up of a non-reactive solid coated with a thin layer of adsorbent material. This is called the stationary phase. The sample is deposited on the plate, which is eluted with a solvent or solvent mixture known as the mobile phase (or eluent). This solvent then moves up the plate via capillary action.
High-performance liquid chromatographyHigh-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a chromatography technique in analytical chemistry used to separate, identify, and quantify specific components (analytes) in mixtures. The mixtures can originate from food, chemicals, pharmaceuticals, biological, environmental and agriculture, etc., in which the sample analyzed is either a liquid or has been dissolved into a liquid.
Glass woolGlass wool is an insulating material made from glass fiber arranged using a binder into a texture similar to wool. The process traps many small pockets of air between the glass, and these small air pockets result in high thermal insulation properties. Glass wool is produced in rolls or slabs, with different thermal and mechanical properties. It may also be produced as a material that can be sprayed or applied in place, on the surface to be insulated.
ElutionIn analytical and organic chemistry, elution is the process of extracting one material from another by washing with a solvent: washing of loaded ion-exchange resins to remove captured ions, or eluting proteins or other biopolymers from an electrophoresis or chromatography column. In a liquid chromatography experiment, for example, an analyte is generally adsorbed by ("bound to") an adsorbent in a liquid chromatography column.
Analytical chemistryAnalytical chemistry (or chemical analysis) is the branch of chemistry concerned with the development and application of methods to identify the chemical composition of materials and quantify the amounts of components in mixtures.
AdsorptionAdsorption is the adhesion of atoms, ions, or molecules from a gas, liquid, or dissolved solid to a surface. This process creates a film of the adsorbate on the surface of the adsorbent. This process differs from absorption, in which a fluid (the absorbate) is dissolved by or permeates a liquid or solid (the absorbent).AnalyteAn analyte, component (in clinical chemistry), titrand (in titrations), or chemical species is a substance or chemical constituent that is of interest in an analytical procedure. The remainder of the sample is called the matrix. The procedure of analysis measures the analyte's chemical or physical properties, thus establishing its identity or concentration in the sample.*As an Amazon Associate I earn from qualifying purchases.