Weigert's elastic stain is a combination of
stains
A stain is an unwanted localized discoloration, often in fabrics or textiles.
Stain(s) or The Stain(s) may also refer to:
Color
* Stain (heraldry), a non-standard tincture
* Staining, in biology, a technique used to highlight contrast in samples
...
used in
histology
Histology,
also known as microscopic anatomy or microanatomy, is the branch of biology that studies the microscopic anatomy of biological tissue (biology), tissues. Histology is the microscopic counterpart to gross anatomy, which looks at large ...
which is useful in identifying
elastic fiber
Elastic fibers (or yellow fibers) are an essential component of the extracellular matrix composed of bundles of proteins (elastin) which are produced by a number of different cell types including fibroblasts, endothelial, smooth muscle, and ai ...
s. Often
orcein
Orcein, also called archil, orchil, lacmus and C.I. Natural Red 28, is any dye extracted from several species of lichen, commonly known as "orchella weeds", found in various parts of the world. A major source is the archil lichen, '' Roccella tin ...
or a combination of
resorcinol
Resorcinol (or resorcin) is a phenolic compound. It is an organic compound with the formula C6H4(OH)2. It is one of three isomeric benzenediols, the 1,3-isomer (or ''meta- (chemistry), meta''-isomer). Resorcinol crystallizes from benzene as co ...
and
fuchsine
Fuchsine (sometimes spelled fuchsin) or rosaniline hydrochloride is a magenta dye with chemical formula C20H19N3·HCl. are used for staining. For counterstaining cell nuclei
nuclear fast red or
hematoxylin
Haematoxylin American and British English spelling differences#ae and oe, or hematoxylin (), also called natural black 1 or Colour Index International, C.I. 75290, is a chemical compound, compound extracted from wood#Heartwood and sapwood, heart ...
is also used. After applying, elastic fibers show up blue colored while cell nuclei show as red or blue.
See also
*
Karl Weigert
Karl Weigert, Carl Weigert (19 March 1845 in Münsterberg in Silesia – 5 August 1904 in Frankfurt am Main) was a German Jewish pathologist. His nephew was Fritz Weigert and his cousin was Paul Ehrlich.
He received his education at the un ...
*
Masson's trichrome stain
External links
*
{{cell-biology-stub
Histology
Staining