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Weigert's elastic stain is a combination of stains used in
histology Histology, also known as microscopic anatomy or microanatomy, is the branch of biology which studies the microscopic anatomy of biological tissues. Histology is the microscopic counterpart to gross anatomy, which looks at larger structures vi ...
which is useful in identifying
elastic fiber Elastic fibers (or yellow fibers) are an essential component of the extracellular matrix composed of bundles of proteins ( elastin) which are produced by a number of different cell types including fibroblasts, endothelial, smooth muscle, and ai ...
s. Often
orcein Orcein, also archil, orchil, lacmus and C.I. Natural Red 28, are names for dyes extracted from several species of lichen, commonly known as "orchella weeds", found in various parts of the world. A major source is the archil lichen, ''Roccella tinc ...
or a combination of
resorcinol Resorcinol (or resorcin) is an organic compound with the formula C6H4(OH)2. It is one of three isomeric benzenediols, the 1,3-isomer (or '' meta''-isomer). Resorcinol crystallizes from benzene as colorless needles that are readily soluble in ...
and
fuchsine Fuchsine (sometimes spelled fuchsin) or rosaniline hydrochloride is a magenta dye with chemical formula C20H19N3·HCl.
are used for staining. For counterstaining cell nuclei nuclear fast red or
hematoxylin Haematoxylin or hematoxylin (), also called natural black 1 or C.I. 75290, is a compound extracted from heartwood of the logwood tree ('' Haematoxylum campechianum'') with a chemical formula of . This naturally derived dye has been used as a ...
is also used. After applying elastic fibers show up blue coloured while cell nuclei gets red or blue.


See also

*
Karl Weigert Karl Weigert, Carl Weigert (19 March 1845 in Münsterberg in Silesia – 5 August 1904 in Frankfurt am Main) was a German Jewish pathologist. His nephew was Fritz Weigert and his cousin was Paul Ehrlich. He received his education at the uni ...
*
Masson's trichrome stain Masson's trichrome is a three-colour staining procedure used in histology. The recipes evolved from Claude L. Pierre Masson's (1880–1959) original formulation have different specific applications, but all are suited for distinguishing cells fr ...


External links

* {{cell-biology-stub Histology Staining