Sudan II (Solvent Orange 7, C.I. 12140, C
18H
16N
2O)
[ is a ]lysochrome A lysochrome is a soluble dye used for histochemical staining of lipids, which include triglycerides, fatty acids, and lipoproteins. Lysochromes such as Sudan IV
Sudan IV (C24H20N4O) is a lysochrome (fat-soluble dye) diazo dye used for the staini ...
(fat-soluble dye) azo dye
Azo dyes are organic compounds bearing the functional group R−N=N−R′, in which R and R′ are usually aryl and substituted aryl groups. They are a commercially important family of azo compounds, i.e. compounds containing the C- ...
used for staining
Staining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently used in histology (microscopic study of biological tissues), in cytology (microscopic study of cells), and in ...
of triglycerides
A triglyceride (TG, triacylglycerol, TAG, or triacylglyceride) is an ester derived from glycerol and three fatty acids (from ''tri-'' and '' glyceride'').
Triglycerides are the main constituents of body fat in humans and other vertebrates, as ...
in frozen sections, and some protein bound lipids
Lipids are a broad group of naturally-occurring molecules which includes fats, waxes, sterols, fat-soluble vitamins (such as vitamins A, D, E and K), monoglycerides, diglycerides, phospholipids, and others. The functions of lipids includ ...
and lipoproteins
A lipoprotein is a biochemical assembly whose primary function is to transport hydrophobic lipid (also known as fat) molecules in water, as in blood plasma or other extracellular fluids. They consist of a triglyceride and cholesterol center, s ...
on paraffin sections. It has the appearance of red powder with melting point 156–158 °C and maximum absorption at 493(420) nm.
Uses
In industry, it is used to color nonpolar substances like oil
An oil is any nonpolar chemical substance that is composed primarily of hydrocarbons and is hydrophobic (does not mix with water) & lipophilic (mixes with other oils). Oils are usually flammable and surface active. Most oils are unsaturate ...
s, fat
In nutrition, biology, and chemistry, fat usually means any ester of fatty acids, or a mixture of such compounds, most commonly those that occur in living beings or in food.
The term often refers specifically to triglycerides (triple es ...
s, waxes, greases, various hydrocarbon
In organic chemistry, a hydrocarbon is an organic compound consisting entirely of hydrogen and carbon. Hydrocarbons are examples of group 14 hydrides. Hydrocarbons are generally colourless and hydrophobic, and their odors are usually weak or ...
products, and acrylic
Acrylic may refer to:
Chemicals and materials
* Acrylic acid, the simplest acrylic compound
* Acrylate polymer, a group of polymers (plastics) noted for transparency and elasticity
* Acrylic resin, a group of related thermoplastic or thermosett ...
emulsion
An emulsion is a mixture of two or more liquids that are normally immiscible (unmixable or unblendable) owing to liquid-liquid phase separation. Emulsions are part of a more general class of two-phase systems of matter called colloids. Althou ...
s.
It was used as food dye under the designation FD&C Red 32 in the US until the FDA banned its use in food in 1956 due to toxicity.
References
{{Stains
Azo dyes
Sudan dyes
2-Naphthols
Alkyl-substituted benzenes