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Surface-enhanced ellipsometric contrast microscopy (SEEC) uses an upright or inverted optical microscope in a crossed Polarization (waves), polarization configuration and specific supporting plates called surfs on which the sample is deposited for observation. It is described as an optical Imaging, nanoscopy technique.
SEEC relies on precise control of the reflection properties of Polarization (waves), polarized light on a surface, improving the axial sensitivity of an optical microscope by two orders of magnitude without reducing its lateral resolution. Applications could include real-time visualization of films as thin as 0.3 micrometers and isolated nano-objects in air and in water.
A 2006 study on polarized light coherence led to the development of new supports (the surfs) having contrast amplification properties for standard optical microscopy in cross-polarizer mode. Made of optical layers on an opaque or transparent substrate, these supports do not modify the Polarization (waves), light polarization after reflection even if the numerical aperture of the incident source is significant. This property is modified when a sample is present on a surf; a non-null light component is then detected after it has been analyzed, rendering the sample visible.
The performance of these supports is evaluated by measuring the contrast (C) of the sample defined as: C = (I1-I0)/(I0+I1) where I0 and I1 represent the intensities reflected by the bare surf and by the analyzed sample on the surf, respectively. For a one nanometer-film thickness, the surfs display a contrast 200 times higher than on silicon wafer.
This high contrast increase allows the visualization with standard Optics, optical microscope of films with thicknesses down to 0.3 nanometers, as well as nano-objects (down to a 2 nanometer diameter) and this, without any kind of sample labeling (neither fluorescence, nor a radioactive marker). An illustration of the contrast enhancement is in the Figure for optical microscopy between cross polarizers of a Langmuir–Blodgett film, Langmuir-Blodgett structure on a silicon wafer and on a surf.
Surface-enhanced ellipsometric contrast microscopy (SEEC) uses an upright or inverted optical microscope in a crossed Polarization (waves), polarization configuration and specific supporting plates called surfs on which the sample is deposited for observation. It is described as an optical Imaging, nanoscopy technique.
SEEC relies on precise control of the reflection properties of Polarization (waves), polarized light on a surface, improving the axial sensitivity of an optical microscope by two orders of magnitude without reducing its lateral resolution. Applications could include real-time visualization of films as thin as 0.3 micrometers and isolated nano-objects in air and in water.
Principles
A 2006 study on polarized light coherence led to the development of new supports (the surfs) having contrast amplification properties for standard optical microscopy in cross-polarizer mode. Made of optical layers on an opaque or transparent substrate, these supports do not modify the Polarization (waves), light polarization after reflection even if the numerical aperture of the incident source is significant. This property is modified when a sample is present on a surf; a non-null light component is then detected after it has been analyzed, rendering the sample visible.
The performance of these supports is evaluated by measuring the contrast (C) of the sample defined as: C = (I1-I0)/(I0+I1) where I0 and I1 represent the intensities reflected by the bare surf and by the analyzed sample on the surf, respectively. For a one nanometer-film thickness, the surfs display a contrast 200 times higher than on silicon wafer.
This high contrast increase allows the visualization with standard Optics, optical microscope of films with thicknesses down to 0.3 nanometers, as well as nano-objects (down to a 2 nanometer diameter) and this, without any kind of sample labeling (neither fluorescence, nor a radioactive marker). An illustration of the contrast enhancement is in the Figure for optical microscopy between cross polarizers of a Langmuir–Blodgett film, Langmuir-Blodgett structure on a silicon wafer and on a surf.
Applications
Life sciences
*Biological films *Biochip, Biochips *Soft lithographyThin films and surface treatment
*Langmuir-Blodgett filmsNano-materials
*Nanoparticles *GrapheneCommercial applications
Nanolane's Sarfus Mapping Station is based on surface-enhanced ellipsometric contrast microscopy.References
{{Optical microscopy Nanotechnology Microscopy Scientific techniques Biological techniques and tools Optical microscopy