phosphoribosyl 1,2-cyclic phosphate 1,2-diphosphodiesterase, known also as Cyclic phosphate dihydrolase is a

hydrolase In biochemistry, hydrolases constitute a class of enzymes that commonly function as biochemical catalysts that use water to break a chemical bond: :\ce \quad \xrightarrowtext\quad \ce This typically results in dividing a larger molecule into s ...

enzyme An enzyme () is a protein that acts as a biological catalyst by accelerating chemical reactions. The molecules upon which enzymes may act are called substrate (chemistry), substrates, and the enzyme converts the substrates into different mol ...

found in various species of

bacteria Bacteria (; : bacterium) are ubiquitous, mostly free-living organisms often consisting of one Cell (biology), biological cell. They constitute a large domain (biology), domain of Prokaryote, prokaryotic microorganisms. Typically a few micr ...

. The enzyme uses

water Water is an inorganic compound with the chemical formula . It is a transparent, tasteless, odorless, and Color of water, nearly colorless chemical substance. It is the main constituent of Earth's hydrosphere and the fluids of all known liv ...

to catalyze the hydrolysis of a cyclic ribophosphate to form alpha-D-ribose 1,5-bisphosphate. Formally, the enzyme catalyzes the following

chemical reaction A chemical reaction is a process that leads to the chemistry, chemical transformation of one set of chemical substances to another. When chemical reactions occur, the atoms are rearranged and the reaction is accompanied by an Gibbs free energy, ...

s, :''alpha-D-ribose 1,2-cyclic phosphate 5-phosphate + H2O = D-ribose 2,5-bisphosphate + H⁺'' :''D-ribose 2,5-bisphosphate + H2O = D-ribose 5-phosphate + phosphate + H⁺'' In ''

Eggerthella lenta ''Eggerthella lenta'' is a Gram-positive, anaerobic species of bacteria which comprises part of the human microbiome. It has been found in the human gastrointestinal tract, female reproductive system and male prostate. Occasionally it can cause d ...

'', '' Gordonibacter urolithinfaciens'' and '' Gordonibacter pamelaeae'', the enzyme is encoded by the gene ''phnPP''. The protein consists of 252

amino acids Amino acids are organic compounds that contain both amino and carboxylic acid functional groups. Although over 500 amino acids exist in nature, by far the most important are the Proteinogenic amino acid, 22 α-amino acids incorporated into p ...

. The enzyme is known to have

metal A metal () is a material that, when polished or fractured, shows a lustrous appearance, and conducts electrical resistivity and conductivity, electricity and thermal conductivity, heat relatively well. These properties are all associated wit ...

ion An ion () is an atom or molecule with a net electrical charge. The charge of an electron is considered to be negative by convention and this charge is equal and opposite to the charge of a proton, which is considered to be positive by convent ...

binding sites for Mn²⁺ and Zn²⁺. The enzyme is involved in the catabolism of organophosphonates.

References

Enzymes {{Hydrolase-stub