Lumír Krejčí
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Lumír Krejčí (born 30 March 1972 in
Slavičín Slavičín () is a town in Zlín District in the Zlín Region of the Czech Republic. It has about 6,200 inhabitants. Administrative division Slavičín consists of four municipal parts (in brackets population according to the 2021 census): *Slavi ...
) is a Czech
biochemist Biochemists are scientists who are trained in biochemistry. They study chemical processes and chemical transformations in living organisms. Biochemists study DNA, proteins and Cell (biology), cell parts. The word "biochemist" is a portmanteau of ...
. His research is focused on regulatory processes involved in maintaining genome integrity. Currently, as Associate Professor in biochemistry, he leads the laboratory of recombination and DNA repair (LORD) at the Department of Biology, Faculty of Medicine, at
Masaryk University Masaryk University (MU) (; ) is the second largest university in the Czech Republic, a member of the Compostela Group and the Utrecht Network. Founded in 1919 in Brno, it now consists of ten faculties and 35,115 students. It is named after To ...
in
Brno Brno ( , ; ) is a Statutory city (Czech Republic), city in the South Moravian Region of the Czech Republic. Located at the confluence of the Svitava (river), Svitava and Svratka (river), Svratka rivers, Brno has about 403,000 inhabitants, making ...
. His laboratory has worked on understanding how Rad51 paralogs act during
homologous recombination Homologous recombination is a type of genetic recombination in which genetic information is exchanged between two similar or identical molecules of double-stranded or single-stranded nucleic acids (usually DNA as in Cell (biology), cellular organi ...
. At Masaryk University, Lumír Krejčí organizes
Mendel Lectures The Mendel Lectures is a series of lectures given by the world's top scientists in genetics, molecular biology, biochemistry, microbiology, medicine and related areas which has been held in the refectory of the St Thomas's Abbey, Brno, Augustian Ab ...
, a series of lectures given by the world´s top scientists, and he is the founders of BIOSKOP - Research Education Center.


Publications

His most cited papers are: *Krejci L, Van Komen S, Li Y, Villemain J, Reddy MS, Klein H, Ellenberger T, Sung P. "DNA helicase Srs2 disrupts the Rad51 presynaptic filament." ''Nature''. 2003 May;423(6937):305. Cited 558 times according to Google Scholar Google Scholar author pag

Accessed Sept. 22, 2019.
*Papouli E, Chen S, Davies AA, Huttner D, Krejci L, Sung P, Ulrich HD. Crosstalk between SUMO and ubiquitin on PCNA is mediated by recruitment of the helicase Srs2p. ''Molecular cell''. 2005 Jul 1;19(1):123-33. Cited 486 times according to Google Scholar *Sung P, Krejci L, Van Komen S, Sehorn MG. Rad51 recombinase and recombination mediators. ''Journal of Biological Chemistry''. 2003 Oct 31;278(44):42729-32. Cited 433 times according to Goggle Scholar


Early life and education

Lumír Krejčí was born in
Slavičín Slavičín () is a town in Zlín District in the Zlín Region of the Czech Republic. It has about 6,200 inhabitants. Administrative division Slavičín consists of four municipal parts (in brackets population according to the 2021 census): *Slavi ...
in the
Zlín Region Zlín Region () is an administrative unit () of the Czech Republic, located in the south-eastern part of the historical region of Moravia. It is named after its capital Zlín. Together with the Olomouc Region it forms a cohesion area of Central M ...
of the
Czech Republic The Czech Republic, also known as Czechia, and historically known as Bohemia, is a landlocked country in Central Europe. The country is bordered by Austria to the south, Germany to the west, Poland to the northeast, and Slovakia to the south ...
. He received his B.Sc and M.Sc degrees in
biochemistry Biochemistry, or biological chemistry, is the study of chemical processes within and relating to living organisms. A sub-discipline of both chemistry and biology, biochemistry may be divided into three fields: structural biology, enzymology, a ...
from
Masaryk University Masaryk University (MU) (; ) is the second largest university in the Czech Republic, a member of the Compostela Group and the Utrecht Network. Founded in 1919 in Brno, it now consists of ten faculties and 35,115 students. It is named after To ...
, in 1993 and 1995, respectively. During his PhD studies at
Masaryk University Masaryk University (MU) (; ) is the second largest university in the Czech Republic, a member of the Compostela Group and the Utrecht Network. Founded in 1919 in Brno, it now consists of ten faculties and 35,115 students. It is named after To ...
, he spent 5 years as a fellow at
Aarhus University Aarhus University (, abbreviated AU) is a public research university. Its main campus is located in Aarhus, Denmark. It is the second largest and second oldest university in Denmark. The university is part of the Coimbra Group, the Guild, and Ut ...
in Denmark under supervision of Christian Bendixen. Afterwards, Krejčí conducted postdoctoral research work between 2001 and 2005 with Patrick Sung, initially at University of Texas Health Science Center at San Antonio (2001-2003), later at Yale University (2003-2005).


Research

Throughout his career, Lumír Krejčí was mainly interested in understanding the mechanisms living organisms use to cope with
genome instability Genome instability (also genetic instability or genomic instability) refers to a high frequency of mutations within the genome of a cellular lineage. These mutations can include changes in nucleic acid sequences, chromosomal rearrangements or ...
, more precisely
homologous recombination Homologous recombination is a type of genetic recombination in which genetic information is exchanged between two similar or identical molecules of double-stranded or single-stranded nucleic acids (usually DNA as in Cell (biology), cellular organi ...
. His key finding during this period concerns the role of Srs2 helicase in dismantling Rad51 nucleofilament in
homologous recombination Homologous recombination is a type of genetic recombination in which genetic information is exchanged between two similar or identical molecules of double-stranded or single-stranded nucleic acids (usually DNA as in Cell (biology), cellular organi ...
. Recently his laboratory focuses also on identification of new anticancer compounds.


Laboratory of Recombination of DNA Repair (LORD)

In 2005, Krejčí moved back to Brno, where he was appointed Principal Investigator, and later in 2012 Associate Professor. The main objectives of his lab’s research are “to decipher the intrinsic functions of
homologous recombination Homologous recombination is a type of genetic recombination in which genetic information is exchanged between two similar or identical molecules of double-stranded or single-stranded nucleic acids (usually DNA as in Cell (biology), cellular organi ...
(HR) which has a dual role in the maintenance of genome stability. First, it promotes the faithful repair of DNA double-strand breaks (DSBs) belonging among of the most lethal forms of
DNA damage DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. A weakened capacity for DNA repair is a risk factor for the development of cancer. DNA is constantly modified ...
. Moreover, HR is responsible for the creation of genetic variability during
meiosis Meiosis () is a special type of cell division of germ cells in sexually-reproducing organisms that produces the gametes, the sperm or egg cells. It involves two rounds of division that ultimately result in four cells, each with only one c ...
by directing the formation of reciprocal crossovers that result in random combinations of alleles and traits.” His main contributions concerns: (i) the effect of post-translational modification of diverse HR proteins by
Sumo protein In molecular biology, SUMO (Small Ubiquitin-like Modifier) proteins are a Protein family, family of small proteins that are covalent bond, covalently attached to and detached from other proteins in cell (biology), cells to modify their function. T ...
(examples include:
Rad52 RAD52 homolog (S. cerevisiae), also known as RAD52, is a protein which in humans is encoded by the ''RAD52'' gene. Function The protein encoded by this gene shares similarity with ''Saccharomyces cerevisiae'' Rad52, a protein important for DN ...
., Rad59,
PCNA Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase delta, DNA polymerase δ in eukaryotic cell (biology), cells and is essential for replication. PCNA is a homotrimer and achieves its ...
); (ii) elucidation of the molecular mechanism of the key step of HR, recombination-associated DNA synthesis; (iii) role of paralogues of
RAD51 DNA repair protein RAD51 homolog 1 is a protein encoded by the gene ''RAD51''. The enzyme encoded by this gene is a member of the RAD51 protein family which assists in repair of DNA double strand breaks. RAD51 family members are homologous to t ...
in promoting RAD51 filament stability Currently, the laboratory focuses on two main topics, development of novel active compounds targeting diverse
nuclease In biochemistry, a nuclease (also archaically known as nucleodepolymerase or polynucleotidase) is an enzyme capable of cleaving the phosphodiester bonds that link nucleotides together to form nucleic acids. Nucleases variously affect single and ...
s and understanding the regulation of RAD51 nucleofilament.


Personal life

Lumír Krejčí is married to Kateřina Krejčí, with whom he has three children.


References

{{DEFAULTSORT:Krejčí, Lumír Czech biochemists Academic staff of Masaryk University 1972 births Living people People from Slavičín