Kinetic capillary electrophoresis or KCE is

capillary electrophoresis Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels. Very often, CE refers to capillary zone electrophoresis (CZE), but other electr ...

molecule A molecule is a group of two or more atoms held together by attractive forces known as chemical bonds; depending on context, the term may or may not include ions which satisfy this criterion. In quantum physics, organic chemistry, and bioch ...

s that interact during electrophoresis. KCE was introduced and developed by Professor Sergey Krylov and his research group at

York University York University (french: Université York), also known as YorkU or simply YU, is a public university, public research university in Toronto, Ontario, Canada. It is Canada's fourth-largest university, and it has approximately 55,700 students, 7,0 ...

, Toronto, Canada. It serves as a conceptual platform for development of

homogeneous Homogeneity and heterogeneity are concepts often used in the sciences and statistics relating to the uniformity of a substance or organism. A material or image that is homogeneous is uniform in composition or character (i.e. color, shape, siz ...

chemical affinity In chemical physics and physical chemistry, chemical affinity is the electronic property by which dissimilar chemical species are capable of forming chemical compounds. Chemical affinity can also refer to the tendency of an atom or compound to comb ...

methods for studies of molecular interactions (measurements of binding and rate constants) and affinity purification (purification of known molecules and search for unknown molecules). Different KCE methods are designed by varying initial and boundary conditions – the way interacting molecules enter and exit the capillary. Several KCE methods were described: non-equilibrium capillary electrophoresis of the equilibrium mixtures (NECEEM), sweeping capillary electrophoresis (SweepCE), and plug-plug KCE (ppKCE).

External links

* More detailed description and several applications of KCE methods (measuring equilibrium and rate constants of molecular interactions, quantitative affinity analysis of proteins, thermochemistry of protein–ligand interactions, selection of

aptamer Aptamers are short sequences of artificial DNA, RNA, XNA, or peptide that bind a specific target molecule, or family of target molecules. They exhibit a range of affinities ( KD in the pM to μM range), with little or no off-target binding ...

s, determination of temperature inside a capillary) can be found in a PDF presentation
KCE is a conceptual platform for kinetic homogeneous affinity methods.

References

{{DEFAULTSORT:Kinetic Capillary Electrophoresis Electrophoresis Chemical kinetics