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Ion interaction chromatography (ion-pair chromatography) is a
Ion interaction chromatography (ion-pair chromatography) is a laboratory technique
A laboratory (; ; colloquially lab) is a facility that provides controlled conditions in which scientific or technological research, experiments, and measurement may be performed. Laboratory services are provided in a variety of settings: physicia ...
for separating ions
An ion () is an atom or molecule with a net electrical charge.
The charge of an electron is considered to be negative by convention and this charge is equal and opposite to the charge of a proton, which is considered to be positive by conve ...
with chromatography
In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it through a system ( ...
. In this technique ions are mixed with ion pairing reagents (IPR). The analyte combines with its reciprocal ion in the IPR, this corresponds to retention time
In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it through a system (a ...
. Often organic salts
In chemistry, a salt is a chemical compound consisting of an ionic assembly of positively charged cations and negatively charged anions, which results in a compound with no net electric charge. A common example is table salt, with positively ...
are selected to pair with solute(s). The formation of this pair affects the interaction of the pair with the mobile phase and the stationary phase.
See also
*Ion association
In chemistry, ion association is a chemical reaction whereby ions of opposite electric charge come together in solution to form a distinct chemical entity. Ion associates are classified, according to the number of ions that associate with each ...
*Intimate ion pair
In chemistry, the intimate ion pair concept, introduced by Saul Winstein, describes the interactions between a cation, anion and surrounding solvent molecules. In ordinary aqueous solutions of inorganic salts, an ion is completely solvated and ...
References
Chromatography {{chromatography-stub