Hanks' salts is a collective group of
salts
In chemistry, a salt or ionic compound is a chemical compound consisting of an assembly of positively charged ions ( cations) and negatively charged ions (anions), which results in a compound with no net electric charge (electrically neutral). ...
rich in
bicarbonate
In inorganic chemistry, bicarbonate (IUPAC-recommended nomenclature: hydrogencarbonate) is an intermediate form in the deprotonation of carbonic acid. It is a polyatomic anion with the chemical formula .
Bicarbonate serves a crucial bioche ...
ions, formulated in 1940 by the microbiologist John H. Hanks. Typically, they are used as a
buffer
Buffer may refer to:
Science
* Buffer gas, an inert or nonflammable gas
* Buffer solution, a solution used to prevent changes in pH
* Lysis buffer, in cell biology
* Metal ion buffer
* Mineral redox buffer, in geology
Technology and engineeri ...
system in
cell culture
Cell culture or tissue culture is the process by which cell (biology), cells are grown under controlled conditions, generally outside of their natural environment. After cells of interest have been Cell isolation, isolated from living tissue, ...
media and aid in maintaining the optimum physiological
pH (roughly 7.0–7.4) for cellular growth. Due to their poorly
reactive nature and small concentration in solution, Hanks' salts are mainly used in media that are exposed to atmospheric conditions as opposed to
incubation. Performing the latter drastically exceeds the buffer capacity of Hanks' salts and may result in cell death.
The recipe according to AATBIO
Table 1. Required components
# Prepare 800 mL of distilled water in a suitable container.
# Add 8 g of NaCl to the solution.
# Add 400 mg of KCl to the solution.
# Add 140 mg of CaCl
2 to the solution.
# Add 100 mg of MgSO
4-7H
2O to the solution.
# Add 100 mg of MgCl
2-6H
2O to the solution.
# Add 60 mg of Na
2HPO
4-2H
2O to the solution.
# Add 60 mg of KH
2PO
4 to the solution.
# Add 1 g of D-Glucose (Dextrose) to the solution.
# Add 350 mg of NaHCO
3 to the solution.
# Add distilled water until volume is 1 L.
References
{{DEFAULTSORT:Hanks' Salts
Buffer solutions
Cell culture media