Dehalococcoides Mccartyi

''Dehalococcoides'' is a genus of bacteria within class Dehalococcoidia that obtain energy via the oxidation of hydrogen and subsequent reductive dehalogenation of halogenated organic compounds in a mode of anaerobic respiration called organohalide respiration. They are well known for their great potential to remediate halogenated ethenes and aromatics. They are the only bacteria known to transform highly chlorinated dioxins, PCBs. In addition, they are the only known bacteria to transform tetrachloroethene ( perchloroethene, PCE) to ethene.

Microbiology

The first member of the genus ''Dehalococcoides'' was described in 1997 as ''Dehalococcoides ethenogenes'' strain 195 (nom. inval.). Additional ''Dehalococcoides'' members were later described as strains CBDB1, BAV1, FL2, VS, and GT. In 2012 all yet-isolated ''Dehalococcoides'' strains were summarized under the new taxonomic name '' D. mccartyi'', with strain 195 as the type strain.

GTDB release 202 clusters the genus into three species, all labeled ''Dehalococcoides mccartyi'' in their NCBI accession.

Activities

''Dehalococcoides'' are obligately organohalide-respiring bacteria, meaning that they can only grow by using halogenated compounds as electron acceptors. Currently, hydrogen (H₂) is often regarded as the only known electron donor to support growth of ''dehalococcoides'' bacteria. However, studies have shown that utilizing various electron donors such as formate, and methyl viologen, have also been effective in promoting growth for various species of ''dehalococcoides''. In order to perform reductive dehalogenation processes, electrons are transferred from electron donors through dehydrogenases, and ultimately utilized to reduce halogenated compounds, many of which are human-synthesized chemicals acting as pollutants. Furthermore, it has been shown that a majority of reductive dehalogenase activities lie within the extracellular and membranous components of ''D. ethenogenes'', indicating that dechlorination processes may function semi-independently from intracellular systems. Currently, all known ''dehalococcoides'' strains require acetate for producing cellular material, however, the underlying mechanisms are not well understood as they appear to lack fundamental enzymes that complete biosynthesis cycles found in other organisms.

''Dehalococcoides'' can transform many highly toxic and/or persistent compounds. This includes tetrachloroethene (PCE) and trichloroethene (TCE) which are transformed to non-toxic ethene, and chlorinated dioxins, vinyl chloride, benzenes, polychlorinated biphenyls (PCBs), phenols and many other aromatic contaminants.

Applications

''Dehalococcoides'' can uniquely transform many highly toxic and/or persistent compounds that are not transformed by any other known bacteria, in addition to halogenated compounds that other common organohalide respirers utilize. For example, common compounds such as chlorinated dioxins, benzenes, PCBs, phenols and many other aromatic substrates can be reduced into less harmful chemical forms. However, ''dehalococcoides'' are currently the only known dechlorinating bacteria with the unique ability to degrade the highly recalcitrant, tetrachloroethene (PCE) and tricholoroethene (TCE) compounds into less-toxic forms that are more suitable for environmental conditions, and thus utilized in bioremediation. Their capacity to grow by using contaminants allows them to proliferate in contaminated soil or groundwater, offering promise for ''in situ'' decontamination efforts.

The process of transforming halogenated pollutants to non-toxic compounds involves different reductive enzymes. ''D. mccartyi'' strain BAV1 is able to reduce vinyl chloride, a toxic contaminant that usually originates from landfills, to ethene by using a special vinyl chloride reductase thought to be coded for by the ''bvcA'' gene. A chlorobenzene reductive dehalogenase has also been identified in the strain CBDB1.

Several companies worldwide now use ''Dehalococcoides''-containing mixed cultures in commercial remediation efforts. In mixed cultures, other bacteria present can augment the dehalogenation process by producing metabolic products that can be used by ''Dehalococcoides'' and others involved in the degradation process. For example, ''Dehalococcoides'' sp. strain WL can work alongside ''Dehalobacter'' in a step-wise manner to degrade vinyl chloride: ''Dehalobacter'' converts 1,1,2-TCA to vinyl chloride, which is subsequently degraded by ''Dehalococcoides''. Also, the addition of electron acceptors is needed - they are converted to hydrogen ''in situ'' by other bacteria present, which can then be used as an electron source by ''Dehalococcoides.'' MEAL (a methanol, ethanol, acetate, and lactate mixture) is documented to have been used as substrate. In the US, BAV1 was patented for the ''in situ'' reductive dechlorination of vinyl chlorides and dichloroethenes in 2007. ''D. mccartyi'' in high-density dechlorinating bioflocs have also been used in ''ex situ'' bioremediation.

Although ''dehalococcoides'' have been shown to reduce contaminants such as PCE and TCE, it appears that individual species have various dechlorinating capabilities which contributes to the degree that these compounds are reduced. This could have implications on the effects of bioremediation tactics. For example, particular strains of ''dehalococcoides'' have shown preference to produce more soluble, carcinogenic intermediates such as 1,2–dichloroethene isomers and vinyl chloride that contrasts against bioremediation goals, primarily due to their harmful nature.
Therefore, an important aspect of current bioremediation tactics involves the utilization of multiple dechlorinating organisms to promote symbiotic relationships within a mixed culture to ensure complete reduction to less-toxic ethene. As a result, studies have focused upon metabolic pathways and environmental factors that regulate reductive dehalogenative processes in order to better implement ''dehalococcoides'' for bioremediation tactics.

However, not all members of ''Dehalococcoides'' can reduce all halogenated contaminants. Certain strains cannot use PCE or TCE as electron acceptors (e.g. CBDB1) and some cannot use vinyl chloride as an electron acceptor (e.g. FL2). ''D. mccartyi s''trains 195 and SFB93 are inhibited by high concentrations of acetylene (which builds up in contaminated groundwater sites as a result of TCE degradation) via changes in gene expression that likely disrupt normal electron transport chain function. When selecting ''Dehalococcoides'' strains for bioremediation use, it is important to consider their metabolic capabilities and their sensitivities to different chemicals.

Genomes

Several strains of ''Dehalococcoides sp.'' has been sequenced. They contain between 14 and 36 reductive dehalogenase homologous (rdh) operons each consisting of a gene for the active dehalogenases (rdhA) and a gene for a putative membrane anchor (rdhB). Most rdh-operons in ''Dehalococcoides'' genomes are preceded by a regulator gene, either of the marR-type (rdhR) or a two-component system (rdhST). ''Dehalococcoides'' have very small genomes of about 1.4-1.5 Mio base pairs. This is one of the smallest value for free-living organisms.

Biochemistry

''Dehalococcoides'' strains do not seem to encode quinones but respire with a novel protein-bound electron transport chain.

See also

* Bioaugmentation
* Bioremediation
* Biostimulation
* List of bacterial orders
* List of bacteria genera

References

External links

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{{Taxonbar, from=Q1182938
Bacteria genera
Bioremediation
Monotypic bacteria genera
Chloroflexota