A bioassay is an analytical method to determine the potency or effect of a substance by its effect on living animals or plants (''in vivo''), or on living cells or tissues (''in vitro''). A bioassay can be either quantal or quantitative, direct or indirect. If the measured response is binary, the assay is quantal; if not, it is quantitative. A bioassay may be used to detect biological hazards or to give an assessment of the quality of a mixture. A bioassay is often used to monitor

water quality Water quality refers to the chemical, physical, and biological characteristics of water based on the standards of its usage. It is most frequently used by reference to a set of standards against which compliance, generally achieved through tr ...

as well as

wastewater Wastewater (or waste water) is water generated after the use of freshwater, raw water, drinking water or saline water in a variety of deliberate applications or processes. Another definition of wastewater is "Used water from any combination of do ...

discharges and its impact on the surroundings. It is also used to assess the environmental impact and safety of new technologies and facilities. Bioassays are essential in pharmaceutical, medical and agricultural sciences for development and launching of new drugs, vitamins, etc.

Principle

A bioassay is a biochemical test to estimate the potency of a sample compound. Usually this potency can only be measured relative to a standard compound. A typical bioassay involves a ''stimulus'' (ex. drugs) applied to a ''subject'' (ex. animals, tissues, plants). The corresponding ''response'' (ex. death) of the subject is thereby triggered and measured.

History

The first use of a bioassay dates back to the late 19th century, when the foundation of bioassays was laid down by German physician Paul Ehrlich. He introduced the concept of standardization by the reactions of living matter. His bioassay on

diphtheria Diphtheria is an infection caused by the bacteria, bacterium ''Corynebacterium diphtheriae''. Most infections are asymptomatic or have a mild Course (medicine), clinical course, but in some outbreaks, the mortality rate approaches 10%. Signs a ...

antitoxin was the first bioassay to receive recognition. His use of bioassay was able to discover that administration of gradually increasing dose of diphtheria in animals stimulated production of antiserum. One well known example of a bioassay is the "canary in the coal mine" experiment. To provide advance warning of dangerous levels of methane in the air, miners would take methane-sensitive canaries into coal mines. If the canary died due to a build-up of methane, the miners would leave the area as quickly as possible. Many early examples of bioassays used animals to test the carcinogenicity of chemicals. In 1915, Yamaigiwa Katsusaburo and Koichi Ichikawa tested the carcinogenicity of coal tar using the inner surface of rabbit's ears. From the 1940s to the 1960s, animal bioassays were primarily used to test the toxicity and safety of drugs, food additives, and pesticides. Beginning in the late 1960s and 1970s, reliance on bioassays increased as public concern for occupational and environmental hazards increased.

Classifications

Bioassay can be classified by how it is applied and how the response is recorded. ; Direct assay : In a direct assay, the stimulus applied to the subject is specific and directly measurable, and the response to that stimulus is recorded. The variable of interest is the specific stimulus required to produce a response of interest (ex. death of the subject). ;Indirect assay :In an indirect assay, the stimulus is fixed in advance and the response is measured in the subjects. The variable of interest in the experiment is the response to a fixed stimulus of interest. ;Quantitative response :The measurement of the response to the stimulus is on a continuous scale (ex. blood sugar content, degree of color change in cell growth medium). ;Quantal response :The response is binary; it is a determination of whether or not an event occurs (ex. death of the subject).

Examples

One classical bioassay is the Ames test. A strain of ''Salmonella'' that requires histidine to grow is put on two plates with growth medium containing minimal amounts of histidine and some rat liver extract (to mimick liver metabolism). A suspected mutagen is added to one plate. If the plate with the suspected mutagen grows more visible colonies, it is probably mutagenic: a mutagen might cause the strain of bacterium to regain the ability to make its own histidine. Most other forms of toxicology testing are also bioassays. Animals or cell cultures may be put under a number of levels of a suspected toxin to ascertain whether the substance causes harmful changes and at what level it does so. The value, a common measure of acute toxicity, describes the dose at which a substance is lethal to 50% of tested animals. The potency of a drug may be measured using a bioassay.

Environmental bioassays

Environmental bioassays are generally a broad-range survey of

toxicity Toxicity is the degree to which a chemical substance or a particular mixture of substances can damage an organism. Toxicity can refer to the effect on a whole organism, such as an animal, bacteria, bacterium, or plant, as well as the effect o ...

. A toxicity identification evaluation is conducted to determine what the relevant toxicants are. Although bioassays are beneficial in determining the biological activity within an organism, they can often be time-consuming and laborious. Organism-specific factors may result in data that are not applicable to others in that species. For these reasons, other biological techniques are often employed, including radioimmunoassays. ''See bioindicator.''

Water pollution Water pollution (or aquatic pollution) is the contamination of Body of water, water bodies, with a negative impact on their uses. It is usually a result of human activities. Water bodies include lakes, rivers, oceans, aquifers, reservoirs and ...

control requirements in the United States require some industrial dischargers and municipal

sewage treatment Sewage treatment is a type of wastewater treatment which aims to remove contaminants from sewage to produce an effluent that is suitable to discharge to the surrounding environment or an intended reuse application, thereby preventing water p ...

plants to conduct bioassays. These procedures, called whole effluent toxicity tests, include acute toxicity tests as well as chronic test methods. The methods involve exposing living aquatic organisms to samples of wastewater for a specific length of time. Another example is the bioassay ECOTOX, which uses the microalgae '' Euglena gracilis'' to test the toxicity of water samples. (''See'' Bioindicator#Microalgae in water quality)

References