Benedict's reagent (often called Benedict's qualitative solution or Benedict's solution) is a chemical
reagent
In chemistry, a reagent ( ) or analytical reagent is a substance or compound added to a system to cause a chemical reaction, or test if one occurs. The terms ''reactant'' and ''reagent'' are often used interchangeably, but reactant specifies a ...
and complex mixture of
sodium carbonate
Sodium carbonate, , (also known as washing soda, soda ash and soda crystals) is the inorganic compound with the formula Na2CO3 and its various hydrates. All forms are white, odourless, water-soluble salts that yield moderately alkaline solutions ...
,
sodium citrate, and
copper(II) sulfate pentahydrate. It is often used in place of
Fehling's solution to detect the presence of
reducing sugars. The presence of other reducing substances also gives a positive result.
[Collins Edexcel International GCSEBiology, Student Book () p.42-43] Such tests that use this reagent are called the Benedict's tests. A positive test with Benedict's reagent is shown by a color change from clear blue to brick-red with a precipitate.
Generally, Benedict's test detects the presence of
aldehydes,
alpha-hydroxy-ketones, and
hemiacetals, including those that occur in certain
ketoses. Thus, although the ketose
fructose
Fructose, or fruit sugar, is a Ketose, ketonic monosaccharide, simple sugar found in many plants, where it is often bonded to glucose to form the disaccharide sucrose. It is one of the three dietary monosaccharides, along with glucose and galacto ...
is not strictly a reducing sugar, it is an alpha-hydroxy-ketone and gives a positive test because the base in the reagent converts it into the
aldose
An aldose is a monosaccharide (a simple sugar) with a carbon backbone chain with a carbonyl group on the endmost carbon atom, making it an aldehyde, and hydroxyl groups connected to all the other carbon atoms. Aldoses can be distinguished from keto ...
s
glucose and
mannose. Oxidation of the reducing sugar by the cupric (Cu
2+) complex of the reagent produces a cuprous (Cu
+), which
precipitates as insoluble red
copper(I) oxide (Cu
2O).
It is named after American chemist
Stanley Rossiter Benedict
Stanley Rossiter Benedict (17 March 1884 – 21 December 1936) was an American chemist best known for discovering Benedict's reagent, a solution that detects certain sugars.
Personal life
Stanley Rossiter Benedict was born on March 17, 1884 to ...
.
Composition and preparation
Benedict's reagent is a deep-blue aqueous solution. Each litre contains:
* 17.3g copper sulfate
* 173g sodium citrate
* 100g anhydrous sodium carbonate or, equivalently, 270g sodium carbonate decahydrate
Separate solutions of the reagents are made. The sodium carbonate and sodium citrate are mixed first, and then the copper sulfate is added slowly with constant stirring.
Sodium citrate acts as a complexing agent which keeps Cu
2+ in solution, since it would otherwise precipitate. Sodium carbonate serves to keep the solution alkaline. In the presence of mild reducing agents, the copper(II) ion is reduced to copper(I), which precipitates in the alkaline conditions as very conspicuous red copper(I) oxide.
Organic analysis
To test for the presence of
monosaccharide
Monosaccharides (from Greek ''monos'': single, '' sacchar'': sugar), also called simple sugars, are the simplest forms of sugar and the most basic units (monomers) from which all carbohydrates are built.
They are usually colorless, water-solub ...
s and reducing
disaccharide sugars in food, the food sample is dissolved in water and a small amount of Benedict's reagent is added. During a
water bath, which is usually 4–10 minutes, the solution should progress through the colors of blue (with no reducing sugar present), orange, yellow, green, red, and then brick red precipitate or brown (if a high concentration of reducing sugar is present). A color change would signify the presence of a reducing sugar.
The common disaccharides lactose and maltose are directly detected by Benedict's reagent because each contains a glucose with a free reducing aldehyde
moiety
Moiety may refer to:
Chemistry
* Moiety (chemistry), a part or functional group of a molecule
** Moiety conservation, conservation of a subgroup in a chemical species
Anthropology
* Moiety (kinship), either of two groups into which a society is ...
after
isomerization.
Sucrose
Sucrose, a disaccharide, is a sugar composed of glucose and fructose subunits. It is produced naturally in plants and is the main constituent of white sugar. It has the molecular formula .
For human consumption, sucrose is extracted and refined ...
(table
sugar
Sugar is the generic name for sweet-tasting, soluble carbohydrates, many of which are used in food. Simple sugars, also called monosaccharides, include glucose, fructose, and galactose. Compound sugars, also called disaccharides or double ...
) contains two sugars (fructose and glucose) joined by their
glycosidic bond in such a way as to prevent the glucose undergoing
isomerization to an aldehyde, or fructose to alpha-hydroxy-ketone form. Sucrose is thus a non-reducing sugar which does not react with Benedict's reagent. However, sucrose indirectly produces a positive result with Benedict's reagent if heated with dilute hydrochloric acid prior to the test, although it is modified during this treatment as the acidic conditions and heat break the glycosidic bond in sucrose through
hydrolysis. The products of sucrose decomposition are glucose and fructose, both of which can be detected by Benedict's reagent as described above.
Starch
Starch or amylum is a polymeric carbohydrate consisting of numerous glucose units joined by glycosidic bonds. This polysaccharide is produced by most green plants for energy storage. Worldwide, it is the most common carbohydrate in human diets ...
es do not react or react very poorly with Benedict's reagent due to the relatively small number of reducing sugar moieties which occur only at the ends of
carbohydrate chains. Other carbohydrates which produce a negative result include
inositol
Inositol, or more precisely ''myo''-inositol, is a carbocyclic sugar that is abundant in the brain and other mammalian tissues; it mediates cell signal transduction in response to a variety of hormones, neurotransmitters, and growth factors and ...
.
Benedict's reagent can also be used to test for the presence of
glucose in
urine, elevated levels of which is known as
glucosuria. Glucosuria can be indicative of
diabetes mellitus, but Benedict's test is not recommended or used for diagnosis of the aforementioned condition. This is due to the possibility of a reaction in which the presence of other reducing substances such as
ascorbic acid
Vitamin C (also known as ascorbic acid and ascorbate) is a water-soluble vitamin found in citrus and other fruits and vegetables, also sold as a dietary supplement and as a topical 'serum' ingredient to treat melasma (dark pigment spots) an ...
, drugs (
levodopa
-DOPA, also known as levodopa and -3,4-dihydroxyphenylalanine, is an amino acid that is made and used as part of the normal biology of some plants and animals, including humans. Humans, as well as a portion of the other animals that utilize -DOPA ...
, contrast used in radiological procedures) and
homogentisic acid (
alkaptonuria) creates a false positive.
As color of the obtained precipitate can be used to infer the quantity of sugar present in the solution, the test is semi-quantitative. A greenish precipitate indicates about 0.5 g% concentration; yellow precipitate indicates 1 g% concentration; orange indicates 1.5 g% concentration; and red indicates 2 g% or higher concentration.
Quantitative reagent
Benedict's
quantitative reagent contains potassium thiocyanate and is used to quantitatively determine the concentration of reducing sugars.
This solution forms a
copper thiocyanate precipitate which is white and can be used in
titration. The titration should be repeated with 1% glucose solution instead of the sample for
calibration.
Net reaction
The net reaction between an
aldehyde (or an
alpha-hydroxy-ketone) and the copper(II) ions in Benedict's solution may be written as:
:.
The hydroxide ions in the equation forms when sodium carbonate dissolves in water. With the citrate included, the reaction becomes:
:.
See also
*
Dextrose equivalent
Other oxidizing reagents
*
Fehling's solution
*
Tollens' reagent
Other reducing reagents
*
Jones reductor
*
Walden reductor
References
{{Organic reactions
Analytical reagents
Chemical tests
Coordination complexes
Copper compounds
Oxidizing agents