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Analytical chemistry studies and uses instruments and methods to separate, identify, and quantify matter. In practice, separation, identification or quantification may constitute the entire analysis or be combined with another method. Separation isolates
analyte An analyte, component (in clinical chemistry), or chemical species is a substance or chemical constituent that is of interest in an analytical procedure. The purest substances are referred to as analytes, such as 24 karat gold, NaCl, water, etc. ...
s. Qualitative analysis identifies analytes, while quantitative analysis determines the numerical amount or concentration. Analytical chemistry consists of classical, wet chemical methods and modern, instrumental methods. Classical qualitative methods use separations such as
precipitation In meteorology, precipitation is any product of the condensation of atmospheric water vapor that falls under gravitational pull from clouds. The main forms of precipitation include drizzle, rain, sleet, snow, ice pellets, graupel and hail. ...
, extraction, and
distillation Distillation, or classical distillation, is the process of separation process, separating the components or substances from a liquid mixture by using selective boiling and condensation, usually inside an apparatus known as a still. Dry distilla ...
. Identification may be based on differences in color, odor, melting point, boiling point, solubility, radioactivity or reactivity. Classical quantitative analysis uses mass or volume changes to quantify amount. Instrumental methods may be used to separate samples using
chromatography In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it through a system ( ...
, electrophoresis or
field flow fractionation Field-flow fractionation, abbreviated FFF, is a separation technique which does not have a stationary phase. It is similar to liquid chromatography as it works on dilute solutions or suspensions of the solute. Separation is achieved by applying ...
. Then qualitative and quantitative analysis can be performed, often with the same instrument and may use light interaction, heat interaction,
electric fields Electric Fields are an Aboriginal Australian electronic music duo made up of vocalist Zaachariaha Fielding and keyboard player and producer Michael Ross. Electric Fields combine modern electric-soul music with Aboriginal culture and sing in Pi ...
or
magnetic fields A magnetic field is a vector field that describes the magnetic influence on moving electric charges, electric currents, and magnetic materials. A moving charge in a magnetic field experiences a force perpendicular to its own velocity and to ...
. Often the same instrument can separate, identify and quantify an analyte. Analytical chemistry is also focused on improvements in
experimental design The design of experiments (DOE, DOX, or experimental design) is the design of any task that aims to describe and explain the variation of information under conditions that are hypothesized to reflect the variation. The term is generally associ ...
,
chemometrics Chemometrics is the science of extracting information from chemical systems by data-driven means. Chemometrics is inherently interdisciplinary, using methods frequently employed in core data-analytic disciplines such as multivariate statistics, a ...
, and the creation of new measurement tools. Analytical chemistry has broad applications to medicine, science, and engineering.


History

Analytical chemistry has been important since the early days of chemistry, providing methods for determining which elements and chemicals are present in the object in question. During this period, significant contributions to analytical chemistry included the development of systematic
elemental analysis Elemental analysis is a process where a sample of some material (e.g., soil, waste or drinking water, bodily fluids, minerals, chemical compounds) is analyzed for its elemental and sometimes isotopic composition. Elemental analysis can be qualita ...
by
Justus von Liebig Justus Freiherr von Liebig (12 May 1803 – 20 April 1873) was a German scientist who made major contributions to agricultural and biological chemistry, and is considered one of the principal founders of organic chemistry. As a professor at t ...
and systematized organic analysis based on the specific reactions of functional groups. The first instrumental analysis was flame emissive spectrometry developed by
Robert Bunsen Robert Wilhelm Eberhard Bunsen (; 30 March 1811 – 16 August 1899) was a German chemist. He investigated emission spectra of heated elements, and discovered caesium (in 1860) and rubidium (in 1861) with the physicist Gustav Kirchhoff. The Bu ...
and
Gustav Kirchhoff Gustav Robert Kirchhoff (; 12 March 1824 – 17 October 1887) was a German physicist who contributed to the fundamental understanding of electrical circuits, spectroscopy, and the emission of black-body radiation by heated objects. He ...
who discovered rubidium (Rb) and
caesium Caesium (IUPAC spelling) (or cesium in American English) is a chemical element with the symbol Cs and atomic number 55. It is a soft, silvery-golden alkali metal with a melting point of , which makes it one of only five elemental metals that a ...
(Cs) in 1860. Most of the major developments in analytical chemistry took place after 1900. During this period, instrumental analysis became progressively dominant in the field. In particular, many of the basic spectroscopic and spectrometric techniques were discovered in the early 20th century and refined in the late 20th century. The separation sciences follow a similar time line of development and also became increasingly transformed into high performance instruments. In the 1970s many of these techniques began to be used together as hybrid techniques to achieve a complete characterization of samples. Starting in the 1970s, analytical chemistry became progressively more inclusive of biological questions (
bioanalytical chemistry Bioanalysis is a sub-discipline of analytical chemistry covering the quantitative measurement of xenobiotics (drugs and their metabolites, and biological molecules in unnatural locations or concentrations) and biotics ( macromolecules, proteins ...
), whereas it had previously been largely focused on inorganic or small organic molecules. Lasers have been increasingly used as probes and even to initiate and influence a wide variety of reactions. The late 20th century also saw an expansion of the application of analytical chemistry from somewhat academic chemical questions to
forensic Forensic science, also known as criminalistics, is the application of science to criminal and civil laws, mainly—on the criminal side—during criminal investigation, as governed by the legal standards of admissible evidence and criminal p ...
,
environmental A biophysical environment is a biotic and abiotic surrounding of an organism or population, and consequently includes the factors that have an influence in their survival, development, and evolution. A biophysical environment can vary in scale f ...
,
industrial Industrial may refer to: Industry * Industrial archaeology, the study of the history of the industry * Industrial engineering, engineering dealing with the optimization of complex industrial processes or systems * Industrial city, a city dominate ...
and
medical Medicine is the science and practice of caring for a patient, managing the diagnosis, prognosis, prevention, treatment, palliation of their injury or disease, and promoting their health. Medicine encompasses a variety of health care practic ...
questions, such as in
histology Histology, also known as microscopic anatomy or microanatomy, is the branch of biology which studies the microscopic anatomy of biological tissues. Histology is the microscopic counterpart to gross anatomy, which looks at larger structures vis ...
. Modern analytical chemistry is dominated by instrumental analysis. Many analytical chemists focus on a single type of instrument. Academics tend to either focus on new applications and discoveries or on new methods of analysis. The discovery of a chemical present in blood that increases the risk of cancer would be a discovery that an analytical chemist might be involved in. An effort to develop a new method might involve the use of a
tunable laser A tunable laser is a laser whose wavelength of operation can be altered in a controlled manner. While all laser gain media allow small shifts in output wavelength, only a few types of lasers allow continuous tuning over a significant wavelength ran ...
to increase the specificity and sensitivity of a spectrometric method. Many methods, once developed, are kept purposely static so that data can be compared over long periods of time. This is particularly true in industrial quality assurance (QA), forensic and environmental applications. Analytical chemistry plays an increasingly important role in the pharmaceutical industry where, aside from QA, it is used in the discovery of new drug candidates and in clinical applications where understanding the interactions between the drug and the patient are critical.


Classical methods

Although modern analytical chemistry is dominated by sophisticated instrumentation, the roots of analytical chemistry and some of the principles used in modern instruments are from traditional techniques, many of which are still used today. These techniques also tend to form the backbone of most undergraduate analytical chemistry educational labs.


Qualitative analysis

Qualitative analysis determines the presence or absence of a particular compound, but not the mass or concentration. By definition, qualitative analyses do not measure quantity.


Chemical tests

There are numerous qualitative chemical tests, for example, the acid test for
gold Gold is a chemical element with the symbol Au (from la, aurum) and atomic number 79. This makes it one of the higher atomic number elements that occur naturally. It is a bright, slightly orange-yellow, dense, soft, malleable, and ductile met ...
and the Kastle-Meyer test for the presence of
blood Blood is a body fluid in the circulatory system of humans and other vertebrates that delivers necessary substances such as nutrients and oxygen to the cells, and transports metabolic waste products away from those same cells. Blood in the c ...
.


Flame test

Inorganic qualitative analysis generally refers to a systematic scheme to confirm the presence of certain aqueous ions or elements by performing a series of reactions that eliminate a range of possibilities and then confirm suspected ions with a confirming test. Sometimes small carbon-containing ions are included in such schemes. With modern instrumentation, these tests are rarely used but can be useful for educational purposes and in fieldwork or other situations where access to state-of-the-art instruments is not available or expedient.


Quantitative analysis

Quantitative analysis is the measurement of the quantities of particular chemical constituents present in a substance. Quantities can be measured by mass (gravimetric analysis) or volume (volumetric analysis).


Gravimetric analysis

The gravimetric analysis involves determining the amount of material present by weighing the sample before and/or after some transformation. A common example used in undergraduate education is the determination of the amount of water in a hydrate by heating the sample to remove the water such that the difference in weight is due to the loss of water.


Volumetric analysis

Titration involves the addition of a reactant to a solution being analyzed until some equivalence point is reached. Often the amount of material in the solution being analyzed may be determined. Most familiar to those who have taken chemistry during secondary education is the acid-base titration involving a color-changing indicator. There are many other types of titrations, for example, potentiometric titrations. These titrations may use different types of indicators to reach some equivalence point.


Instrumental methods


Spectroscopy

Spectroscopy measures the interaction of the molecules with
electromagnetic radiation In physics, electromagnetic radiation (EMR) consists of waves of the electromagnetic field, electromagnetic (EM) field, which propagate through space and carry momentum and electromagnetic radiant energy. It includes radio waves, microwaves, inf ...
. Spectroscopy consists of many different applications such as
atomic absorption spectroscopy Atomic absorption spectroscopy (AAS) and atomic emission spectroscopy (AES) is a spectroanalytical procedure for the quantitative determination of chemical elemlight) by free atoms in the gaseous state. Atomic absorption spectroscopy is based o ...
,
atomic emission spectroscopy Atomic may refer to: * Of or relating to the atom, the smallest particle of a chemical element that retains its chemical properties * Atomic physics, the study of the atom * Atomic Age, also known as the "Atomic Era" * Atomic scale, distances com ...
, ultraviolet-visible spectroscopy,
X-ray spectroscopy X-ray spectroscopy is a general term for several spectroscopic techniques for characterization of materials by using x-ray radiation. Characteristic X-ray spectroscopy When an electron from the inner shell of an atom is excited by the energy o ...
,
fluorescence spectroscopy Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light, that excites the electron ...
,
infrared spectroscopy Infrared spectroscopy (IR spectroscopy or vibrational spectroscopy) is the measurement of the interaction of infrared radiation with matter by absorption, emission, or reflection. It is used to study and identify chemical substances or function ...
, Raman spectroscopy, dual polarization interferometry,
nuclear magnetic resonance spectroscopy Nuclear magnetic resonance spectroscopy, most commonly known as NMR spectroscopy or magnetic resonance spectroscopy (MRS), is a spectroscopic technique to observe local magnetic fields around atomic nuclei. The sample is placed in a magnetic fie ...
,
photoemission spectroscopy Photoemission spectroscopy (PES), also known as photoelectron spectroscopy, refers to energy measurement of electrons emitted from solids, gases or liquids by the photoelectric effect, in order to determine the binding energies of electrons in th ...
,
Mössbauer spectroscopy Mössbauer spectroscopy is a spectroscopic technique based on the Mössbauer effect. This effect, discovered by Rudolf Mössbauer (sometimes written "Moessbauer", German: "Mößbauer") in 1958, consists of the nearly recoil-free emission and abs ...
and so on.


Mass spectrometry

Mass spectrometry measures mass-to-charge ratio of molecules using
electric Electricity is the set of physical phenomena associated with the presence and motion of matter that has a property of electric charge. Electricity is related to magnetism, both being part of the phenomenon of electromagnetism, as described by ...
and
magnetic field A magnetic field is a vector field that describes the magnetic influence on moving electric charges, electric currents, and magnetic materials. A moving charge in a magnetic field experiences a force perpendicular to its own velocity and to ...
s. There are several ionization methods: electron ionization, chemical ionization,
electrospray ionization Electrospray ionization (ESI) is a technique used in mass spectrometry to produce ions using an electrospray in which a high voltage is applied to a liquid to create an aerosol. It is especially useful in producing ions from macromolecules becaus ...
, fast atom bombardment, matrix assisted laser desorption/ionization, and others. Also, mass spectrometry is categorized by approaches of mass analyzers:
magnetic-sector A sector instrument is a general term for a class of mass spectrometer that uses a static electric (E) or magnetic (B) sector or some combination of the two (separately in space) as a mass analyzer. Popular combinations of these sectors have been ...
,
quadrupole mass analyzer The quadrupole mass analyzer, originally conceived by Nobel Laureate Wolfgang Paul and his student Helmut Steinwedel, also known as quadrupole mass filter, is one type of mass analyzer used in mass spectrometry. As the name implies, it consists o ...
,
quadrupole ion trap A quadrupole ion trap or paul trap is a type of ion trap that uses dynamic electric fields to trap charged particles. They are also called radio frequency (RF) traps or Paul traps in honor of Wolfgang Paul, who invented the device and shared the ...
,
time-of-flight Time of flight (ToF) is the measurement of the time taken by an object, particle or wave (be it acoustic, electromagnetic, etc.) to travel a distance through a medium. This information can then be used to measure velocity or path length, or as a w ...
,
Fourier transform ion cyclotron resonance Fourier-transform ion cyclotron resonance mass spectrometry is a type of mass analyzer (or mass spectrometer) for determining the mass-to-charge ratio (''m''/''z'') of ions based on the ion cyclotron resonance, cyclotron frequency of the ions in a ...
, and so on.


Electrochemical analysis

Electroanalytical methods measure the
potential Potential generally refers to a currently unrealized ability. The term is used in a wide variety of fields, from physics to the social sciences to indicate things that are in a state where they are able to change in ways ranging from the simple r ...
(
volts The volt (symbol: V) is the unit of electric potential, electric potential difference (voltage), and electromotive force in the International System of Units (SI). It is named after the Italian physicist Alessandro Volta (1745–1827). Defini ...
) and/or
current Currents, Current or The Current may refer to: Science and technology * Current (fluid), the flow of a liquid or a gas ** Air current, a flow of air ** Ocean current, a current in the ocean *** Rip current, a kind of water current ** Current (stre ...
( amps) in an
electrochemical cell An electrochemical cell is a device capable of either generating electrical energy from chemical reactions or using electrical energy to cause chemical reactions. The electrochemical cells which generate an electric current are called voltaic o ...
containing the analyte. These methods can be categorized according to which aspects of the cell are controlled and which are measured. The four main categories are
potentiometry A potentiometer is an instrument for measuring voltage or 'potential difference' by comparison of an unknown voltage with a known reference voltage. If a sensitive indicating instrument is used, very little current is drawn from the source of the ...
(the difference in electrode potentials is measured),
coulometry Coulometry determines the amount of matter transformed during an electrolysis reaction by measuring the amount of electricity (in coulombs) consumed or produced. It can be used for precision measurements of charge, and the amperes even used to ha ...
(the transferred charge is measured over time),
amperometry Amperometry in chemistry is detection of ions in a solution based on electric current or changes in electric current. Amperometry is used in electrophysiology to study vesicle release events using a carbon fiber electrode. Unlike patch clamp tec ...
(the cell's current is measured over time), and
voltammetry Voltammetry is a category of electroanalytical methods used in analytical chemistry and various industrial processes. In voltammetry, information about an analyte is obtained by measuring the current as the potential is varied. The analytical data ...
(the cell's current is measured while actively altering the cell's potential).


Thermal analysis

Calorimetry and thermogravimetric analysis measure the interaction of a material and
heat In thermodynamics, heat is defined as the form of energy crossing the boundary of a thermodynamic system by virtue of a temperature difference across the boundary. A thermodynamic system does not ''contain'' heat. Nevertheless, the term is al ...
.


Separation

Separation processes are used to decrease the complexity of material mixtures.
Chromatography In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it through a system ( ...
, electrophoresis and
field flow fractionation Field-flow fractionation, abbreviated FFF, is a separation technique which does not have a stationary phase. It is similar to liquid chromatography as it works on dilute solutions or suspensions of the solute. Separation is achieved by applying ...
are representative of this field.


Hybrid techniques

Combinations of the above techniques produce a "hybrid" or "hyphenated" technique. Several examples are in popular use today and new hybrid techniques are under development. For example,
gas chromatography-mass spectrometry Gas is one of the four fundamental states of matter (the others being solid, liquid, and plasma). A pure gas may be made up of individual atoms (e.g. a noble gas like neon), elemental molecules made from one type of atom (e.g. oxygen), or ...
, gas chromatography-
infrared spectroscopy Infrared spectroscopy (IR spectroscopy or vibrational spectroscopy) is the measurement of the interaction of infrared radiation with matter by absorption, emission, or reflection. It is used to study and identify chemical substances or function ...
,
liquid chromatography-mass spectrometry A liquid is a nearly incompressible fluid that conforms to the shape of its container but retains a (nearly) constant volume independent of pressure. As such, it is one of the four fundamental states of matter (the others being solid, gas, a ...
, liquid chromatography-
NMR spectroscopy Nuclear magnetic resonance spectroscopy, most commonly known as NMR spectroscopy or magnetic resonance spectroscopy (MRS), is a spectroscopic technique to observe local magnetic fields around atomic nuclei. The sample is placed in a magnetic fiel ...
, liquid chromatography-infrared spectroscopy, and capillary electrophoresis-mass spectrometry. Hyphenated separation techniques refer to a combination of two (or more) techniques to detect and separate chemicals from solutions. Most often the other technique is some form of
chromatography In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it through a system ( ...
. Hyphenated techniques are widely used in
chemistry Chemistry is the science, scientific study of the properties and behavior of matter. It is a natural science that covers the Chemical element, elements that make up matter to the chemical compound, compounds made of atoms, molecules and ions ...
and
biochemistry Biochemistry or biological chemistry is the study of chemical processes within and relating to living organisms. A sub-discipline of both chemistry and biology, biochemistry may be divided into three fields: structural biology, enzymology and ...
. A
slash Slash may refer to: * Slash (punctuation), the "/" character Arts and entertainment Fictional characters * Slash (Marvel Comics) * Slash (''Teenage Mutant Ninja Turtles'') Music * Harry Slash & The Slashtones, an American rock band * Nash ...
is sometimes used instead of
hyphen The hyphen is a punctuation mark used to join words and to separate syllables of a single word. The use of hyphens is called hyphenation. ''Son-in-law'' is an example of a hyphenated word. The hyphen is sometimes confused with dashes (figure d ...
, especially if the name of one of the methods contains a hyphen itself.


Microscopy

The visualization of single molecules, single cells, biological tissues, and
nanomaterial * Nanomaterials describe, in principle, materials of which a single unit is sized (in at least one dimension) between 1 and 100 nm (the usual definition of nanoscale). Nanomaterials research takes a materials science-based approach to nan ...
s is an important and attractive approach in analytical science. Also, hybridization with other traditional analytical tools is revolutionizing analytical science. Microscopy can be categorized into three different fields:
optical microscopy Optics is the branch of physics that studies the behaviour and properties of light, including its interactions with matter and the construction of instruments that use or detect it. Optics usually describes the behaviour of visible, ultravio ...
, electron microscopy, and scanning probe microscopy. Recently, this field is rapidly progressing because of the rapid development of the computer and camera industries.


Lab-on-a-chip

Devices that integrate (multiple) laboratory functions on a single chip of only millimeters to a few square centimeters in size and that are capable of handling extremely small fluid volumes down to less than picoliters.


Errors

Error can be defined as numerical difference between observed value and true value. The experimental error can be divided into two types, systematic error and random error. Systematic error results from a flaw in equipment or the design of an experiment while random error results from uncontrolled or uncontrollable variables in the experiment. In error the true value and observed value in chemical analysis can be related with each other by the equation : \varepsilon_ = , x - \bar, where * \varepsilon_ is the absolute error. * x is the true value. * \bar is the observed value. An error of a measurement is an inverse measure of accurate measurement, i.e. smaller the error greater the accuracy of the measurement. Errors can be expressed relatively. Given the relative error(\varepsilon_): :\varepsilon_ = \frac = \left , \frac \right , The percent error can also be calculated: :\varepsilon_ \times 100\% If we want to use these values in a function, we may also want to calculate the error of the function. Let f be a function with N variables. Therefore, the
propagation of uncertainty In statistics, propagation of uncertainty (or propagation of error) is the effect of variables' uncertainties (or errors, more specifically random errors) on the uncertainty of a function based on them. When the variables are the values of exp ...
must be calculated in order to know the error in f: :\varepsilon_ (f) \approx \sum_^N \left , \frac \right , \varepsilon_(x_i) = \left , \frac \right , \varepsilon_(x_1) + \left , \frac \right , \varepsilon_(x_2) + \ldots + \left , \frac \right , \varepsilon_(x_N)


Standards


Standard curve

A general method for analysis of concentration involves the creation of a
calibration curve In analytical chemistry, a calibration curve, also known as a standard curve, is a general method for determining the concentration of a substance in an unknown sample by comparing the unknown to a set of standard samples of known concentration. ...
. This allows for the determination of the amount of a chemical in a material by comparing the results of an unknown sample to those of a series of known standards. If the concentration of element or compound in a sample is too high for the detection range of the technique, it can simply be diluted in a pure solvent. If the amount in the sample is below an instrument's range of measurement, the method of addition can be used. In this method, a known quantity of the element or compound under study is added, and the difference between the concentration added and the concentration observed is the amount actually in the sample.


Internal standards

Sometimes an
internal standard An internal standard in analytical chemistry is a chemical substance that is added in a constant amount to samples, the blank and calibration standards in a chemical analysis. This substance can then be used for calibration by plotting the ratio of ...
is added at a known concentration directly to an analytical sample to aid in quantitation. The amount of analyte present is then determined relative to the internal standard as a calibrant. An ideal internal standard is an isotopically enriched analyte which gives rise to the method of
isotope dilution Isotope dilution analysis is a method of determining the quantity of chemical substances. In its most simple conception, the method of isotope dilution comprises the addition of known amounts of isotopically enriched substance to the analyzed samp ...
.


Standard addition

The method of standard addition is used in instrumental analysis to determine the concentration of a substance (
analyte An analyte, component (in clinical chemistry), or chemical species is a substance or chemical constituent that is of interest in an analytical procedure. The purest substances are referred to as analytes, such as 24 karat gold, NaCl, water, etc. ...
) in an unknown sample by comparison to a set of samples of known concentration, similar to using a
calibration curve In analytical chemistry, a calibration curve, also known as a standard curve, is a general method for determining the concentration of a substance in an unknown sample by comparing the unknown to a set of standard samples of known concentration. ...
. Standard addition can be applied to most analytical techniques and is used instead of a
calibration curve In analytical chemistry, a calibration curve, also known as a standard curve, is a general method for determining the concentration of a substance in an unknown sample by comparing the unknown to a set of standard samples of known concentration. ...
to solve the
matrix effect In chemical analysis, matrix refers to the components of a sample other than the analyte of interest. The matrix can have a considerable effect on the way the analysis is conducted and the quality of the results are obtained; such effects are calle ...
problem.


Signals and noise

One of the most important components of analytical chemistry is maximizing the desired signal while minimizing the associated
noise Noise is unwanted sound considered unpleasant, loud or disruptive to hearing. From a physics standpoint, there is no distinction between noise and desired sound, as both are vibrations through a medium, such as air or water. The difference arise ...
. The analytical figure of merit is known as the
signal-to-noise ratio Signal-to-noise ratio (SNR or S/N) is a measure used in science and engineering that compares the level of a desired signal to the level of background noise. SNR is defined as the ratio of signal power to the noise power, often expressed in deci ...
(S/N or SNR). Noise can arise from environmental factors as well as from fundamental physical processes.


Thermal noise

Thermal noise results from the motion of charge carriers (usually electrons) in an electrical circuit generated by their thermal motion. Thermal noise is
white noise In signal processing, white noise is a random signal having equal intensity at different frequencies, giving it a constant power spectral density. The term is used, with this or similar meanings, in many scientific and technical disciplines, ...
meaning that the power
spectral density The power spectrum S_(f) of a time series x(t) describes the distribution of power into frequency components composing that signal. According to Fourier analysis, any physical signal can be decomposed into a number of discrete frequencies, ...
is constant throughout the
frequency spectrum The power spectrum S_(f) of a time series x(t) describes the distribution of power into frequency components composing that signal. According to Fourier analysis, any physical signal can be decomposed into a number of discrete frequencies, ...
. The
root mean square In mathematics and its applications, the root mean square of a set of numbers x_i (abbreviated as RMS, or rms and denoted in formulas as either x_\mathrm or \mathrm_x) is defined as the square root of the mean square (the arithmetic mean of the ...
value of the thermal noise in a resistor is given by :v_ = \sqrt , where ''k''B is
Boltzmann's constant The Boltzmann constant ( or ) is the proportionality factor that relates the average relative kinetic energy of particles in a gas with the thermodynamic temperature of the gas. It occurs in the definitions of the kelvin and the gas constant ...
, ''T'' is the
temperature Temperature is a physical quantity that expresses quantitatively the perceptions of hotness and coldness. Temperature is measured with a thermometer. Thermometers are calibrated in various temperature scales that historically have relied o ...
, ''R'' is the resistance, and \Delta f is the
bandwidth Bandwidth commonly refers to: * Bandwidth (signal processing) or ''analog bandwidth'', ''frequency bandwidth'', or ''radio bandwidth'', a measure of the width of a frequency range * Bandwidth (computing), the rate of data transfer, bit rate or thr ...
of the frequency f.


Shot noise

Shot noise is a type of
electronic noise In electronics, noise is an unwanted disturbance in an electrical signal. Noise generated by electronic devices varies greatly as it is produced by several different effects. In particular, noise is inherent in physics, and central to the ...
that occurs when the finite number of particles (such as
electron The electron ( or ) is a subatomic particle with a negative one elementary electric charge. Electrons belong to the first generation of the lepton particle family, and are generally thought to be elementary particles because they have no kn ...
s in an electronic circuit or
photon A photon () is an elementary particle that is a quantum of the electromagnetic field, including electromagnetic radiation such as light and radio waves, and the force carrier for the electromagnetic force. Photons are massless, so they always ...
s in an optical device) is small enough to give rise to statistical fluctuations in a signal. Shot noise is a
Poisson process In probability, statistics and related fields, a Poisson point process is a type of random mathematical object that consists of points randomly located on a mathematical space with the essential feature that the points occur independently of one ...
, and the charge carriers that make up the current follow a
Poisson distribution In probability theory and statistics, the Poisson distribution is a discrete probability distribution that expresses the probability of a given number of events occurring in a fixed interval of time or space if these events occur with a known co ...
. The root mean square current fluctuation is given by :i_ = \sqrt where ''e'' is the elementary charge and ''I'' is the average current. Shot noise is white noise.


Flicker noise

Flicker noise is electronic noise with a 1/''ƒ'' frequency spectrum; as ''f'' increases, the noise decreases. Flicker noise arises from a variety of sources, such as impurities in a conductive channel, generation, and recombination noise in a
transistor upright=1.4, gate (G), body (B), source (S) and drain (D) terminals. The gate is separated from the body by an insulating layer (pink). A transistor is a semiconductor device used to Electronic amplifier, amplify or electronic switch, switch e ...
due to base current, and so on. This noise can be avoided by
modulation In electronics and telecommunications, modulation is the process of varying one or more properties of a periodic waveform, called the ''carrier signal'', with a separate signal called the ''modulation signal'' that typically contains informatio ...
of the signal at a higher frequency, for example, through the use of a
lock-in amplifier A lock-in amplifier is a type of amplifier that can extract a signal with a known carrier wave from an extremely noisy environment. Depending on the dynamic reserve of the instrument, signals up to a million times smaller than noise components, p ...
.


Environmental noise

Environmental noise Environmental noise is an accumulation of noise pollution that occurs outside. This noise can be caused by transport, industrial, and recreational activities. Noise is frequently described as 'unwanted sound'. Within this context, environmenta ...
arises from the surroundings of the analytical instrument. Sources of electromagnetic noise are
power lines Electric power transmission is the bulk movement of electrical energy from a generating site, such as a power plant, to an electrical substation. The interconnected lines that facilitate this movement form a ''transmission network''. This is d ...
, radio and television stations,
wireless device Wireless communication (or just wireless, when the context allows) is the transfer of information between two or more points without the use of an electrical conductor, optical fiber or other continuous guided medium for the transfer. The most ...
s,
compact fluorescent lamp A compact fluorescent lamp (CFL), also called compact fluorescent light, energy-saving light and compact fluorescent tube, is a fluorescent lamp designed to replace an incandescent light bulb; some types fit into light fixtures designed for inca ...
s and
electric motor An electric motor is an Electric machine, electrical machine that converts electrical energy into mechanical energy. Most electric motors operate through the interaction between the motor's magnetic field and electric current in a Electromagneti ...
s. Many of these noise sources are narrow bandwidth and, therefore, can be avoided. Temperature and
vibration isolation Vibration isolation is the process of isolating an object, such as a piece of equipment, from the source of vibrations. Vibration is undesirable in many domains, primarily engineered systems and habitable spaces, and methods have been developed to p ...
may be required for some instruments.


Noise reduction

Noise reduction can be accomplished either in
computer hardware Computer hardware includes the physical parts of a computer, such as the computer case, case, central processing unit (CPU), Random-access memory, random access memory (RAM), Computer monitor, monitor, Computer mouse, mouse, Computer keyboard, ...
or
software Software is a set of computer programs and associated documentation and data. This is in contrast to hardware, from which the system is built and which actually performs the work. At the lowest programming level, executable code consists ...
. Examples of hardware noise reduction are the use of
shielded cable A shielded cable or screened cable is an electrical cable that has a common conductive layer around its conductors for electromagnetic shielding. This shield is usually covered by an outermost layer of the cable. Common types of cable shieldi ...
,
analog filter Analogue filters are a basic building block of signal processing much used in electronics. Amongst their many applications are the separation of an audio signal before application to bass, mid-range, and tweeter loudspeakers; the combining and ...
ing, and signal modulation. Examples of software noise reduction are
digital filter In signal processing, a digital filter is a system that performs mathematical operations on a sampled, discrete-time signal to reduce or enhance certain aspects of that signal. This is in contrast to the other major type of electronic filter, t ...
ing,
ensemble average In physics, specifically statistical mechanics, an ensemble (also statistical ensemble) is an idealization consisting of a large number of virtual copies (sometimes infinitely many) of a system, considered all at once, each of which represents ...
, boxcar average, and
correlation In statistics, correlation or dependence is any statistical relationship, whether causal or not, between two random variables or bivariate data. Although in the broadest sense, "correlation" may indicate any type of association, in statistics ...
methods.


Applications

Analytical chemistry has applications including in
forensic science Forensic science, also known as criminalistics, is the application of science to criminal and civil laws, mainly—on the criminal side—during criminal investigation, as governed by the legal standards of admissible evidence and criminal ...
,
bioanalysis Bioanalysis is a sub-discipline of analytical chemistry covering the quantitative measurement of xenobiotics (drugs and their metabolites, and biological molecules in unnatural locations or concentrations) and biotics ( macromolecules, protein ...
, clinical analysis, environmental analysis, and
materials analysis Material is a substance or mixture of substances that constitutes an object. Materials can be pure or impure, living or non-living matter. Materials can be classified on the basis of their physical and chemical properties, or on their geologic ...
. Analytical chemistry research is largely driven by performance (sensitivity,
detection limit The limit of detection (LOD or LoD) is the lowest signal, or the lowest corresponding quantity to be determined (or extracted) from the signal, that can be observed with a sufficient degree of confidence or statistical significance. However, the ...
, selectivity, robustness,
dynamic range Dynamic range (abbreviated DR, DNR, or DYR) is the ratio between the largest and smallest values that a certain quantity can assume. It is often used in the context of signals, like sound and light. It is measured either as a ratio or as a base-1 ...
,
linear range The linear range is that range of input or output values for which an electronic amplifier produces an output signal that is a direct, linear function of the input signal. That is, the output can be represented by the equation: :''Output'' = ''Inp ...
, accuracy, precision, and speed), and cost (purchase, operation, training, time, and space). Among the main branches of contemporary analytical atomic spectrometry, the most widespread and universal are optical and mass spectrometry. In the direct elemental analysis of solid samples, the new leaders are laser-induced breakdown and laser ablation mass spectrometry, and the related techniques with transfer of the laser ablation products into
inductively coupled plasma An inductively coupled plasma (ICP) or transformer coupled plasma (TCP) is a type of plasma source in which the energy is supplied by electric currents which are produced by electromagnetic induction, that is, by time-varying magnetic fields. Ope ...
. Advances in design of diode lasers and optical parametric oscillators promote developments in fluorescence and ionization spectrometry and also in absorption techniques where uses of optical cavities for increased effective absorption pathlength are expected to expand. The use of plasma- and laser-based methods is increasing. An interest towards absolute (standardless) analysis has revived, particularly in emission spectrometry. Great effort is being put into shrinking the analysis techniques to
chip Chromatin immunoprecipitation (ChIP) is a type of immunoprecipitation experimental technique used to investigate the interaction between proteins and DNA in the cell. It aims to determine whether specific proteins are associated with specific genom ...
size. Although there are few examples of such systems competitive with traditional analysis techniques, potential advantages include size/portability, speed, and cost. (micro total analysis system (µTAS) or
lab-on-a-chip A lab-on-a-chip (LOC) is a device that integrates one or several laboratory functions on a single integrated circuit (commonly called a "chip") of only millimeters to a few square centimeters to achieve automation and high-throughput screening. ...
).
Microscale chemistry Microscale chemistry (often referred to as small-scale chemistry, in German: Chemie im Mikromaßstab) is an analytical method and also a teaching method widely used at school and at university levels, working with small quantities of chemical su ...
reduces the amounts of chemicals used. Many developments improve the analysis of biological systems. Examples of rapidly expanding fields in this area are
genomics Genomics is an interdisciplinary field of biology focusing on the structure, function, evolution, mapping, and editing of genomes. A genome is an organism's complete set of DNA, including all of its genes as well as its hierarchical, three-dim ...
,
DNA sequencing DNA sequencing is the process of determining the nucleic acid sequence – the order of nucleotides in DNA. It includes any method or technology that is used to determine the order of the four bases: adenine, guanine, cytosine, and thymine. Th ...
and related research in
genetic fingerprinting DNA profiling (also called DNA fingerprinting) is the process of determining an individual's DNA characteristics. DNA analysis intended to identify a species, rather than an individual, is called DNA barcoding. DNA profiling is a forensic t ...
and DNA microarray; proteomics, the analysis of protein concentrations and modifications, especially in response to various stressors, at various developmental stages, or in various parts of the body,
metabolomics Metabolomics is the scientific study of chemical processes involving metabolites, the small molecule substrates, intermediates, and products of cell metabolism. Specifically, metabolomics is the "systematic study of the unique chemical fingerprin ...
, which deals with metabolites;
transcriptomics Transcriptomics technologies are the techniques used to study an organism's transcriptome, the sum of all of its RNA transcripts. The information content of an organism is recorded in the DNA of its genome and expressed through transcription. H ...
, including mRNA and associated fields;
lipidomics Lipidomics is the large-scale study of pathways and networks of cellular lipids in biological systems The word " lipidome" is used to describe the complete lipid profile within a cell, tissue, organism, or ecosystem and is a subset of the "metabol ...
- lipids and its associated fields; peptidomics - peptides and its associated fields; and metallomics, dealing with metal concentrations and especially with their binding to proteins and other molecules. Analytical chemistry has played a critical role in the understanding of basic science to a variety of practical applications, such as biomedical applications, environmental monitoring, quality control of industrial manufacturing, forensic science, and so on. The recent developments in computer automation and information technologies have extended analytical chemistry into a number of new biological fields. For example, automated DNA sequencing machines were the basis for completing human genome projects leading to the birth of
genomics Genomics is an interdisciplinary field of biology focusing on the structure, function, evolution, mapping, and editing of genomes. A genome is an organism's complete set of DNA, including all of its genes as well as its hierarchical, three-dim ...
. Protein identification and peptide sequencing by mass spectrometry opened a new field of proteomics. In addition to automating specific processes, there is effort to automate larger sections of lab testing, such as in companies like
Emerald Cloud Lab Emerald Cloud Lab (ECL) is a privately-owned biotech startup. The company focuses on advancing laboratory virtualization, for chemistry and biotechnology, by building the first fully functional cloud lab, allowing scientists to conduct all of their ...
and Transcriptic. Analytical chemistry has been an indispensable area in the development of nanotechnology. Surface characterization instruments,
electron microscopes An electron microscope is a microscope that uses a beam of accelerated electrons as a source of illumination. As the wavelength of an electron can be up to 100,000 times shorter than that of visible light photons, electron microscopes have a hi ...
and scanning probe microscopes enable scientists to visualize atomic structures with chemical characterizations.


See also

* Important publications in analytical chemistry *
List of chemical analysis methods A list of chemical analysis methods with acronyms. {{Compact ToC, name=no, center=no, top=yes, seealso=yes A * Atomic absorption spectroscopy (AAS) * Atomic emission spectroscopy (AES) * Atomic fluorescence spectroscopy (AFS) * Alpha particle ...
*
List of materials analysis methods This is a list of analysis methods used in materials science. Analysis methods are listed by their acronym, if one exists. Symbols * μSR – see muon spin spectroscopy * χ – see magnetic susceptibility A * AAS – Atomic absorption spec ...
* Measurement uncertainty * Metrology *
Sensory analysis Sensory analysis (or sensory evaluation) is a scientific discipline that applies principles of experimental design and statistical analysis to the use of human senses ( sight, smell, taste, touch and hearing Hearing, or auditory percepti ...
- in the field of
Food science Food science is the basic science and applied science of food; its scope starts at overlap with agricultural science and nutritional science and leads through the scientific aspects of food safety and food processing, informing the development ...
*
Virtual instrumentation Virtual instrumentation is the use of customizable software and modular measurement hardware to create user-defined measurement systems, ''called virtual instruments''. Traditional hardware instrumentation systems are made up of fixed hardware co ...
*
Microanalysis Microanalysis is the chemical identification and quantitative analysis of very small amounts of chemical substances (generally less than 10 mg or 1 ml) or very small surfaces of material (generally less than 1 cm2). One of the pioneer ...
* Quality of analytical results *
Working range Each instrument used in analytical chemistry has a useful working range. This is the range of concentration (or mass) that can be adequately determined by the instrument, where the instrument provides a useful signal that can be related to the con ...


References


Further reading

* Gurdeep, Chatwal Anand (2008). ''Instrumental Methods of Chemical Analysis'' Himalaya Publishing House (India) * Ralph L. Shriner, Reynold C. Fuson, David Y. Curtin, Terence C. Morill: ''The systematic identification of organic compounds - a laboratory manual'', Verlag Wiley, New York 1980, 6. edition, . *Bettencourt da Silva, R; Bulska, E; Godlewska-Zylkiewicz, B; Hedrich, M; Majcen, N; Magnusson, B; Marincic, S; Papadakis, I; Patriarca, M; Vassileva, E; Taylor, P; Analytical measurement: measurement uncertainty and statistics, 2012, .


External links

*
Infografik
an
animation
showing the progress of analytical chemistry
aas
Atomic Absorption Spectrophotometer {{Authority control Materials science