2-''C''-Methyl-D-erythritol 2,4-cyclodiphosphate synthase (MEcPP synthase, IspF, EC 4.6.1.12) is a

zinc Zinc is a chemical element with the symbol Zn and atomic number 30. Zinc is a slightly brittle metal at room temperature and has a shiny-greyish appearance when oxidation is removed. It is the first element in group 12 (IIB) of the periodic t ...

-dependent

enzyme Enzymes () are proteins that act as biological catalysts by accelerating chemical reactions. The molecules upon which enzymes may act are called substrate (chemistry), substrates, and the enzyme converts the substrates into different molecule ...

and a member of the

YgbB N terminal protein domain In molecular biology, YgbB is a protein domain. This entry makes reference to a number of proteins from eukaryotes and prokaryotes which share this common N-terminal signature and appear to be involved in terpenoid biosynthesis. The YgbB protein ...

, which participates in the

MEP pathway The non-mevalonate pathway—also appearing as the mevalonate-independent pathway and the 2-''C''-methyl-D-erythritol 4-phosphate/1-deoxy-D-xylulose 5-phosphate (MEP/DOXP) pathway—is an alternative metabolic pathway for the biosynthesis of the i ...

(non-mevalonate pathway) of

isoprenoid The terpenoids, also known as isoprenoids, are a class of naturally occurring organic chemicals derived from the 5-carbon compound isoprene and its derivatives called terpenes, diterpenes, etc. While sometimes used interchangeably with "terpenes" ...

precursor biosynthesis. It

catalyzes Catalysis () is the process of increasing the rate of a chemical reaction by adding a substance known as a catalyst (). Catalysts are not consumed in the reaction and remain unchanged after it. If the reaction is rapid and the catalyst recycl ...

the following reaction: :4-diphosphocytidyl-2-''C''-methyl-D)erythritol 2-phosphate

\rightleftharpoons

2-''C''-methyl-D-erythritol 2,4-cyclodiphosphate + CMP The enzyme is considered a phosphorus-oxygen lyase. The

systematic name A systematic name is a name given in a systematic way to one unique group, organism, object or chemical substance, out of a specific population or collection. Systematic names are usually part of a nomenclature. A semisystematic name or semitrivial ...

of this enzyme class is 2-phospho-4-(cytidine 5′-diphospho)-2-''C''-methyl-D-D-erythritol CMP-lyase (cyclizing; 2-''C''-methyl-D-erythritol 2,4-cyclodiphosphate-forming). Other names in common use include IspF, YgbB and MEcPP synthase.

Structural studies

As of late 2007, 20

structures A structure is an arrangement and organization of interrelated elements in a material object or system, or the object or system so organized. Material structures include man-made objects such as buildings and machines and natural objects such a ...

have been solved for this class of enzymes, with

PDB PDB may refer to: * Chess Problem Database Server (PDB Server) * 1,4-Dichlorobenzene (paradichlorobenzene) * Party of German-speaking Belgians, (German: '), a political party and predecessor of the ProDG * PDB (Palm OS), a container format for reco ...

accession codes , , , , , , , , , , , , , , , , , , , and .

References

EC 4.6.1 Enzymes of known structure {{lyase-stub