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Pilus
A pilus (Latin for 'hair'; : pili) is a hair-like cell-surface appendage found on many bacteria and archaea. The terms ''pilus'' and '' fimbria'' (Latin for 'fringe'; plural: ''fimbriae'') can be used interchangeably, although some researchers reserve the term ''pilus'' for the appendage required for bacterial conjugation. All conjugative pili are primarily composed of pilin – fibrous proteins, which are oligomeric. Dozens of these structures can exist on the bacterial and archaeal surface. Some bacteria, viruses or bacteriophages attach to receptors on pili at the start of their reproductive cycle. Pili are antigenic. They are also fragile and constantly replaced, sometimes with pili of different composition, resulting in altered antigenicity. Specific host responses to old pili structures are not effective on the new structure. Recombination between genes of some (but not all) pili code for variable (V) and constant (C) regions of the pili (similar to immunoglobulin ...
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Bacteria
Bacteria (; : bacterium) are ubiquitous, mostly free-living organisms often consisting of one Cell (biology), biological cell. They constitute a large domain (biology), domain of Prokaryote, prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit the air, soil, water, Hot spring, acidic hot springs, radioactive waste, and the deep biosphere of Earth's crust. Bacteria play a vital role in many stages of the nutrient cycle by recycling nutrients and the nitrogen fixation, fixation of nitrogen from the Earth's atmosphere, atmosphere. The nutrient cycle includes the decomposition of cadaver, dead bodies; bacteria are responsible for the putrefaction stage in this process. In the biological communities surrounding hydrothermal vents and cold seeps, extremophile bacteria provide the nutrients needed to sustain life by converting dissolved compounds, suc ...
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Plasmid
A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently. They are most commonly found as small circular, double-stranded DNA molecules in bacteria and archaea; however plasmids are sometimes present in and eukaryotic organisms as well. Plasmids often carry useful genes, such as those involved in antibiotic resistance, virulence, secondary metabolism and bioremediation. While chromosomes are large and contain all the essential genetic information for living under normal conditions, plasmids are usually very small and contain additional genes for special circumstances. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms. In the laboratory, plasmids may be introduced into a cell via transformation. Synthetic plasmids are available for procurement over the internet by various vendors ...
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Cell-surface Appendage
An appendage (or outgrowth) is an external body part or natural prolongation that protrudes from an organism's body such as an arm or a leg. Protrusions from single-celled bacteria and archaea are known as cell-surface appendages or surface appendages. In many kinds of eukaryotic cells, the protrusions are known as membrane protrusions or cell appendages (examples include microvilli and cilia). Types in animals In arthropods, an appendage refers to any of the homologous body parts that may extend from a body segment, including antennae, mouthparts (including mandibles, maxillae and maxillipeds), gills, locomotor legs (pereiopods for walking, and pleopods for swimming), sexual organs (gonopods), and parts of the tail (uropods). Typically, each body segment carries one pair of appendages. An appendage which is modified to assist in feeding is known as a maxilliped or gnathopod. In annelids lateral protrusions from the body are called parapodia. In echinoderms an appendage ...
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Pilin
Pilin refers to a class of fibrous proteins that are found in pilus structures in bacteria. These structures can be used for the exchange of genetic material, or as a cell adhesion mechanism. Although not all bacteria have pili or fimbriae, bacterial pathogens often use their fimbriae to attach to host cells. In Gram-negative bacteria, where pili are more common, individual pilin molecules are linked by noncovalent protein-protein interactions, while Gram-positive bacteria often have polymerized LPXTG pilin. Type IV pilin Type IV pilin proteins are α+β proteins characterized by a very long N-terminal alpha helix. The assembly of these pili relies on interactions between the N-terminal helices of the individual monomers. The pilus structure sequesters the helices in the center of the fiber lining a central pore, while antiparallel beta sheets occupy the exterior of the fiber. Role of ComP pilin in bacterial transformation Genetic transformation is the process by whic ...
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Appendage
An appendage (or outgrowth) is an external body part or natural prolongation that protrudes from an organism's body such as an arm or a leg. Protrusions from single-celled bacteria and archaea are known as cell-surface appendages or surface appendages. In many kinds of eukaryotic cells, the protrusions are known as membrane protrusions or cell appendages (examples include microvilli and cilia). Types in animals In arthropods, an appendage refers to any of the homologous body parts that may extend from a body segment, including antennae, mouthparts (including mandibles, maxillae and maxillipeds), gills, locomotor legs ( pereiopods for walking, and pleopods for swimming), sexual organs ( gonopods), and parts of the tail (uropods). Typically, each body segment carries one pair of appendages. An appendage which is modified to assist in feeding is known as a maxilliped or gnathopod. In annelids lateral protrusions from the body are called parapodia. In echinoderms ...
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Fertility Factor (bacteria)
The F-plasmid (first named F by one of its discoverers Esther Lederberg;also called the sex factor in ''E. coli'',the F sex factor, the fertility factor, or simply the F factor) allows genes to be transferred from one bacterium carrying the factor to another bacterium lacking the factor by conjugation. The F factor was the first plasmid to be discovered. Unlike other plasmids, F factor is constitutive for transfer proteins due to a mutation in the gene ''finO''. The F plasmid belongs to F-like plasmids, a class of conjugative plasmids that control sexual functions of bacteria with a fertility inhibition (Fin) system. Discovery Esther M. Lederberg and Luigi L. Cavalli-Sforza discovered "F," subsequently publishing with Joshua Lederberg. Once her results were announced, two other labs joined the studies. "This was not a simultaneous independent discovery of F (I named this as Fertility Factor until it was understood.) We wrote to Hayes, Jacob, & Wollman who then proceeded ...
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Bacterial Conjugation
Bacterial conjugation is the transfer of genetic material between Bacteria, bacterial cells by direct cell-to-cell contact or by a bridge-like connection between two cells. This takes place through a pilus. It is a parasexual cycle, parasexual mode of reproduction in bacteria. It is a mechanism of horizontal gene transfer as are Transformation (genetics), transformation and Transduction (genetics), transduction although these two other mechanisms do not involve cell-to-cell contact. Classical ''E. coli'' bacterial conjugation is often regarded as the bacterial equivalent of sexual reproduction or mating, since it involves the exchange of genetic material. However, it is not sexual reproduction, since no exchange of gamete occurs, and indeed no biogenesis, generation of a new organism: instead, an existing organism is transformed. During classical ''E. coli'' conjugation, the ''donor'' cell provides a conjugative or mobilizable genetic element that is most often a plasmid or trans ...
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Mating Bridge
A mating bridge, also known as a conjugation or population bridge, is a connection between two bacterial cells that provides a passageway for DNA in bacterial conjugation. A mating bridge is different from a sex pilus, which is a structure made by an F+ strain bacterium in bacterial conjugation. The pili (plural) act as attachment sites that promote the binding of bacteria to each other. In this way, an F+ strain makes physical contact with an F− strain. Once contact is made, the pili shorten and thereby draw the donor and recipient cells closer together. A conjugation bridge is then formed between the two cells, which provides a passageway for DNA Deoxyribonucleic acid (; DNA) is a polymer composed of two polynucleotide chains that coil around each other to form a double helix. The polymer carries genetic instructions for the development, functioning, growth and reproduction of al ... transfer. References {{Reflist Bacteriology Organelles ...
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Conjugation
Conjugation or conjugate may refer to: Linguistics *Grammatical conjugation, the modification of a verb from its basic form *Emotive conjugation or Russell's conjugation, the use of loaded language Mathematics *Complex conjugation, the change of sign of the imaginary part of a complex number *Conjugate (square roots), the change of sign of a square root in an expression *Conjugate element (field theory), a generalization of the preceding conjugations to roots of a polynomial of any degree *Conjugate transpose, the complex conjugate of the transpose of a matrix *Harmonic conjugate in complex analysis *Conjugate (graph theory), an alternative term for a line graph, i.e. a graph representing the edge adjacencies of another graph *In group theory, various notions are called conjugation: **Inner automorphism, a type of conjugation homomorphism **Conjugacy class in group theory, related to matrix similarity in linear algebra **Conjugation (group theory), the image of an element under ...
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Nanometer
330px, Different lengths as in respect to the Molecule">molecular scale. The nanometre (international spelling as used by the International Bureau of Weights and Measures; SI symbol: nm), or nanometer (American spelling Despite the various list of dialects of English, English dialects spoken from country to country and within different regions of the same country, there are only slight regional variations in English orthography, the two most notable variati ...), is a units of measurement, unit of length in the International System of Units (SI), equal to one billionth (short scale) or one thousand million (long scale) of a metre, meter (0.000000001 m) and to 1000 picometres. One nanometre can be expressed in scientific notation as 1 × 10−9 m and as  m. History The nanometre was formerly known as the "''millimicrometre''" – or, more commonly, the "''millimicron''" for short – since it is of a micrometre, micrometer. It was often de ...
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