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High-throughput Screening
High-throughput screening (HTS) is a method for scientific discovery especially used in drug discovery and relevant to the fields of biology, materials science and chemistry. Using robotics, data processing/control software, liquid handling devices, and sensitive detectors, high-throughput screening allows a researcher to quickly conduct millions of chemical, genetic, or pharmacological tests. Through this process one can quickly recognize active compounds, antibodies, or genes that modulate a particular biomolecular pathway. The results of these experiments provide starting points for drug design and for understanding the noninteraction or role of a particular location. Assay plate preparation The key labware or testing vessel of HTS is the microtiter plate, which is a small container, usually disposable and made of plastic, that features a grid of small, open divots called ''wells''. In general, microplates for HTS have either 96, 192, 384, 1536, 3456 or 6144 wells. These are a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chemical Genomics Robot
A chemical substance is a unique form of matter with constant chemical composition and characteristic Chemical property, properties. Chemical substances may take the form of a single chemical element, element or chemical compounds. If two or more chemical substances can be combined without Chemical reaction, reacting, they may form a chemical mixture. If a mixture is separated to isolate one chemical substance to a desired degree, the resulting substance is said to be Chemical purity, chemically pure. Chemical substances can exist in several different physical State of matter, states or Phase (matter), phases (e.g. solids, liquids, gases, or plasma (physics), plasma) without changing their chemical composition. Substances Phase transition, transition between these phases of matter in response to changes in temperature or pressure. Some chemical substances can be combined or converted into new substances by means of chemical reactions. Chemicals that do not possess this ability ar ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Embryo
An embryo ( ) is the initial stage of development for a multicellular organism. In organisms that reproduce sexually, embryonic development is the part of the life cycle that begins just after fertilization of the female egg cell by the male sperm cell. The resulting fusion of these two cells produces a single-celled zygote that undergoes many cell divisions that produce cells known as blastomeres. The blastomeres (4-cell stage) are arranged as a solid ball that when reaching a certain size, called a morula, (16-cell stage) takes in fluid to create a cavity called a blastocoel. The structure is then termed a blastula, or a blastocyst in mammals. The mammalian blastocyst hatches before implantating into the endometrial lining of the womb. Once implanted the embryo will continue its development through the next stages of gastrulation, neurulation, and organogenesis. Gastrulation is the formation of the three germ layers that will form all of the different parts of t ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Royal Society Of Chemistry
The Royal Society of Chemistry (RSC) is a learned society and professional association in the United Kingdom with the goal of "advancing the chemistry, chemical sciences". It was formed in 1980 from the amalgamation of the Chemical Society, the Royal Institute of Chemistry, the Faraday Society, and the Society for Analytical Chemistry with a new Royal Charter and the dual role of learned society and professional body. At its inception, the Society had a combined membership of 49,000 in the world. The headquarters of the Society are at Burlington House, Piccadilly, London. It also has offices in Thomas Graham House in Cambridge (named after Thomas Graham (chemist), Thomas Graham, the first president of the Chemical Society) where ''RSC Publishing'' is based. The Society has offices in the United States, on the campuses of The University of Pennsylvania and Drexel University, at the University City Science Center in Philadelphia, Pennsylvania, in both Beijing and Shanghai, People' ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Lab On A Chip (journal)
''Lab on a Chip'' is a peer-reviewed scientific journal which publishes original (primary) research and review articles on any aspect of miniaturisation at the micro and nano scale. ''Lab on a Chip'' is published twice monthly by the Royal Society of Chemistry (RSC) and the editor-in-chief is Aaron Wheeler (University of Toronto). The journal was established in 2001 and hosts other RSC publications: '' Highlights in Chemical Technology'' and '' Highlights in Chemical Biology''. According to the ''Journal Citation Reports'', the journal has a 2023 impact factor of 6.1. Subject coverage ''Lab on a Chip'' publishes research at the micro- and nano-scale across a variety of disciplines including chemistry, biology, bioengineering, physics, electronics, clinical science, chemical engineering, and materials science focusing on lab on a chip technologies. Article types ''Lab on a Chip'' publishes full research papers, urgent communications, critical and tutorial reviews. ''Chips ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Hill Coefficient
In biochemistry and pharmacology, the Hill equation refers to two closely related equations that reflect the binding of ligands to macromolecules, as a function of the ligand concentration. A ligand is "a substance that forms a complex with a biomolecule to serve a biological purpose", and a macromolecule is a very large molecule, such as a protein, with a complex structure of components. Protein-ligand binding typically changes the structure of the target protein, thereby changing its function in a cell. The distinction between the two Hill equations is whether they measure ''occupancy'' or ''response''. The Hill equation reflects the occupancy of macromolecules: the fraction that is saturated or bound by the ligand.For clarity, this article will use the International Union of Basic and Clinical Pharmacology convention of distinguishing between the Hill-Langmuir equation (for receptor saturation) and Hill equation (for tissue response) This equation is formally equivalent to the ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
T-statistic
In statistics, the ''t''-statistic is the ratio of the difference in a number’s estimated value from its assumed value to its standard error. It is used in hypothesis testing via Student's ''t''-test. The ''t''-statistic is used in a ''t''-test to determine whether to support or reject the null hypothesis. It is very similar to the z-score but with the difference that ''t''-statistic is used when the sample size is small or the population standard deviation is unknown. For example, the ''t''-statistic is used in estimating the population mean from a sampling distribution of sample means if the population standard deviation is unknown. It is also used along with p-value when running hypothesis tests where the p-value tells us what the odds are of the results to have happened. Definition and features Let \hat\beta be an estimator of parameter ''β'' in some statistical model. Then a ''t''-statistic for this parameter is any quantity of the form : t_ = \frac, where ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
SSMD
In statistics, the strictly standardized mean difference (SSMD) is a measure of effect size. It is the mean divided by the standard deviation of a difference between two random values each from one of two groups. It was initially proposed for quality control and hit selection in high-throughput screening (HTS) and has become a statistical parameter measuring effect sizes for the comparison of any two groups with random values. Background In high-throughput screening (HTS), quality control (QC) is critical. An important QC characteristic in a HTS assay is how much the positive controls, test compounds, and negative controls differ from one another. This QC characteristic can be evaluated using the comparison of two well types in HTS assays. Signal-to-noise ratio (S/N), signal-to-background ratio (S/B), and the Z-factor have been adopted to evaluate the quality of HTS assays through the comparison of two investigated types of wells. However, the S/B does not take into account any ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Z-factor
The Z-factor is a measure of statistical effect size. It has been proposed for use in high-throughput screening (HTS), where it is also known as Z-prime, to judge whether the response in a particular assay is large enough to warrant further attention. Background In HTS, experimenters often compare a large number (hundreds of thousands to tens of millions) of single measurements of unknown samples to positive and negative control samples. The particular choice of experimental conditions and measurements is called an assay. Large screens are expensive in time and resources. Therefore, prior to starting a large screen, smaller test (or pilot) screens are used to assess the quality of an assay, in an attempt to predict if it would be useful in a high-throughput setting. The Z-factor is an attempt to quantify the suitability of a particular assay for use in a full-scale HTS. Definition Z-factor The Z-factor is defined in terms of four parameters: the means (\mu) and standard dev ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
SiRNA
Small interfering RNA (siRNA), sometimes known as short interfering RNA or silencing RNA, is a class of double-stranded non-coding RNA molecules, typically 20–24 base pairs in length, similar to microRNA (miRNA), and operating within the RNA interference (RNAi) pathway. It interferes with the expression of specific genes with complementary nucleotide sequences by degrading messenger RNA (mRNA) after transcription, preventing translation. Text was copied from this source, which is available under Creative Commons Attribution 4.0 International License It was discovered in 1998 by Andrew Fire at the Carnegie Institution for Science in Washington, D.C. and Craig Mello at the University of Massachusetts in Worcester. Structure Naturally occurring siRNAs have a well-defined structure that is a short (usually 20 to 24- bp) double-stranded RNA (dsRNA) with phosphorylated 5' ends and hydroxylated 3' ends with two overhanging nucleotides. The Dicer enzyme catalyzes prod ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Small Molecule
In molecular biology and pharmacology, a small molecule or micromolecule is a low molecular weight (≤ 1000 daltons) organic compound that may regulate a biological process, with a size on the order of 1 nm. Many drugs are small molecules; the terms are equivalent in the literature. Larger structures such as nucleic acids and proteins, and many polysaccharides are not small molecules, although their constituent monomers (ribo- or deoxyribonucleotides, amino acids, and monosaccharides, respectively) are often considered small molecules. Small molecules may be used as research tools to probe biological function as well as leads in the development of new therapeutic agents. Some can inhibit a specific function of a protein or disrupt protein–protein interactions. Pharmacology usually restricts the term "small molecule" to molecules that bind specific biological macromolecules and act as an effector, altering the activity or function of the target. Small molecules can ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Robot
A robot is a machine—especially one Computer program, programmable by a computer—capable of carrying out a complex series of actions Automation, automatically. A robot can be guided by an external control device, or the robot control, control may be embedded within. Robots may be constructed to evoke Humanoid robot, human form, but most robots are task-performing machines, designed with an emphasis on stark functionality, rather than expressive aesthetics. Robots can be autonomous robot, autonomous or semi-autonomous and range from humanoids such as Honda's ''Advanced Step in Innovative Mobility'' (ASIMO) and TOSY's ''TOSY Ping Pong Playing Robot'' (TOPIO) to industrial robots, robot-assisted surgery, medical operating robots, patient assist robots, dog therapy robots, collectively programmed Swarm robotics, ''swarm'' robots, UAV drones such as General Atomics MQ-1 Predator, and even microscopic Nanorobotics, nanorobots. By mimicking a lifelike appearance or automating mo ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
