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Three-photon Microscopy
Three-photon microscopy (3PEF) is a high-resolution fluorescence microscopy based on nonlinear excitation effect. Different from two-photon excitation microscopy, it uses three exciting photons. It typically uses 1300 nm or longer wavelength lasers to excite the fluorescent dyes with three simultaneously absorbed photons. The fluorescent dyes then emit one photon whose energy is (slightly smaller than) three times the energy of each incident photon. Compared to two-photon microscopy, three-photon microscopy reduces the fluorescence away from the focal plane by 1/z^4, which is much faster than that of two-photon microscopy by 1/z^2. In addition, three-photon microscopy employs near-infrared light with less tissue scattering effect. This causes three-photon microscopy to have higher resolution than conventional microscopy. Concept Three-photon excited fluorescence was first observed by Singh and Bradley in 1964 when they estimated the three-photon absorption cross section of ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorescence
Fluorescence is one of two kinds of photoluminescence, the emission of light by a substance that has absorbed light or other electromagnetic radiation. When exposed to ultraviolet radiation, many substances will glow (fluoresce) with colored visible light. The color of the light emitted depends on the chemical composition of the substance. Fluorescent materials generally cease to glow nearly immediately when the radiation source stops. This distinguishes them from the other type of light emission, phosphorescence. Phosphorescent materials continue to emit light for some time after the radiation stops. This difference in duration is a result of quantum spin effects. Fluorescence occurs when a photon from incoming radiation is absorbed by a molecule, exciting it to a higher energy level, followed by the emission of light as the molecule returns to a lower energy state. The emitted light may have a longer wavelength and, therefore, a lower photon energy than the absorbed radi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chromophores
A chromophore is the part of a molecule responsible for its color. The word is derived . The color that is seen by our eyes is that of the light not absorbed by the reflecting object within a certain wavelength spectrum of visible light. The chromophore is a region in the molecule where the energy difference between two separate molecular orbitals falls within the range of the visible spectrum (or in informal contexts, the spectrum under scrutiny). Visible light that hits the chromophore can thus be absorbed by exciting an electron from its ground state into an excited state. In biological molecules that serve to capture or detect light energy, the chromophore is the moiety that causes a conformational change in the molecule when hit by light. Conjugated pi-bond system chromophores Just like how two adjacent p-orbitals in a molecule will form a pi-bond, three or more adjacent p-orbitals in a molecule can form a conjugated pi-system. In a conjugated pi-system, electrons ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cell Imaging
Cell most often refers to: * Cell (biology), the functional basic unit of life * Cellphone, a phone connected to a cellular network * Clandestine cell, a penetration-resistant form of a secret or outlawed organization * Electrochemical cell, a device used to convert chemical energy to electrical energy * Prison cell, a room used to hold people in prisons Cell may also refer to: Arts, entertainment, and media Fictional entities * Cell (comics), a Marvel comic book character * Cell (''Dragon Ball''), a character in the manga series ''Dragon Ball'' Literature * ''Cell'' (novel), a 2006 horror novel by Stephen King * "Cells", poem, about a hungover soldier in gaol, by Rudyard Kipling * ''The Cell'' (play), an Australian play by Robert Wales Music * Cell (music), a small rhythmic and melodic design that can be isolated, or can make up one part of a thematic context * Cell (American band) * Cell (Japanese band) * ''Cell'' (album), a 2004 album by Plastic Tree * ''Cells'', a 1 ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorescence Techniques
Fluorescence is one of two kinds of photoluminescence, the emission of light by a substance that has absorbed light or other electromagnetic radiation. When exposed to ultraviolet radiation, many substances will glow (fluoresce) with colored visible light. The color of the light emitted depends on the chemical composition of the substance. Fluorescent materials generally cease to glow nearly immediately when the radiation source stops. This distinguishes them from the other type of light emission, phosphorescence. Phosphorescent materials continue to emit light for some time after the radiation stops. This difference in duration is a result of quantum spin effects. Fluorescence occurs when a photon from incoming radiation is absorbed by a molecule, exciting it to a higher energy level, followed by the emission of light as the molecule returns to a lower energy state. The emitted light may have a longer wavelength and, therefore, a lower photon energy than the absorbed radi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Nonlinear Optics
Nonlinear optics (NLO) is the branch of optics that describes the behaviour of light in Nonlinearity, nonlinear media, that is, media in which the polarization density P responds non-linearly to the electric field E of the light. The non-linearity is typically observed only at very high light intensities (when the electric field of the light is >108 V/m and thus comparable to the atomic electric field of ~1011 V/m) such as those provided by lasers. Above the Schwinger limit, the vacuum itself is expected to become nonlinear. In nonlinear optics, the superposition principle no longer holds. History The first nonlinear optical effect to be predicted was two-photon absorption, by Maria Goeppert Mayer for her PhD in 1931, but it remained an unexplored theoretical curiosity until 1961 and the almost simultaneous observation of two-photon absorption at Bell Labs and the discovery of second-harmonic generation by Peter Franken ''et al.'' at University of Michigan, both shortly after th ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Laser Scanning
Laser scanning is the controlled Deflection (physics), deflection of laser beams, visible or invisible. Scanned laser beams are used in some 3-D printers, in rapid prototyping, in machines for material processing, in laser engraving machines, in ophthalmological laser systems for the treatment of presbyopia, in confocal microscopy, in laser printers, in laser shows, in Laser TV, and in barcode scanners. Applications specific to mapping and 3D object reconstruction are known as ''3D laser scanner''. Technology Scanning mirrors Most laser scanners use moveable mirrors to steer the laser beam. The steering of the beam can be ''one-dimensional'', as inside a laser printer, or ''two-dimensional'', as in a laser show system. Additionally, the mirrors can lead to a ''periodic'' motion - like the rotating ''polygon mirror'' in a barcode scanner or so-called ''resonant galvanometer'' scanners - or to a ''freely addressable'' motion, as in servo-controlled galvanometer scanners. One al ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Isomerization
In chemistry, isomerization or isomerisation is the process in which a molecule, polyatomic ion or molecular fragment is transformed into an isomer with a different chemical structure. Enolization is an example of isomerization, as is tautomerization. When the activation energy for the isomerization reaction is sufficiently small, both isomers can often be observed and the equilibrium ratio will shift in a temperature-dependent equilibrium with each other. Many values of the standard free energy difference, \Delta G^\circ, have been calculated, with good agreement between observed and calculated data. Examples and applications Alkanes Skeletal isomerization occurs in the cracking process, used in the petrochemical industry to convert straight chain alkanes to isoparaffins as exemplified in the conversion of normal octane to 2,5-dimethylhexane (an "isoparaffin"): : Fuels containing branched hydrocarbons are favored for internal combustion engines for their higher octan ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Field Of View
The field of view (FOV) is the angle, angular extent of the observable world that is visual perception, seen at any given moment. In the case of optical instruments or sensors, it is a solid angle through which a detector is sensitive to electromagnetic radiation. It is further relevant in ''angle of view, photography''. Humans and animals In the context of human and primate vision, the term "field of view" is typically only used in the sense of a restriction to what is visible by external apparatus, like when wearing spectacles or virtual reality goggles. Note that eye movements are allowed in the definition but do not change the field of view when understood this way. If the analogy of the eye's retina working as a sensor is drawn upon, the corresponding concept in human (and much of animal vision) is the visual field. It is defined as "the number of degrees of visual angle during stable fixation of the eyes".Strasburger, Hans; Pöppel, Ernst (2002). Visual Field. In G. A ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Full Width At Half Maximum
In a distribution, full width at half maximum (FWHM) is the difference between the two values of the independent variable at which the dependent variable is equal to half of its maximum value. In other words, it is the width of a spectrum curve measured between those points on the ''y''-axis which are half the maximum amplitude. Half width at half maximum (HWHM) is half of the FWHM if the function is symmetric. The term full duration at half maximum (FDHM) is preferred when the independent variable is time. FWHM is applied to such phenomena as the duration of pulse waveforms and the spectral width of sources used for optical communications and the resolution of spectrometers. The convention of "width" meaning "half maximum" is also widely used in signal processing to define bandwidth as "width of frequency range where less than half the signal's power is attenuated", i.e., the power is at least half the maximum. In signal processing terms, this is at most −3 dB of att ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mouse Brain
A mouse (: mice) is a small rodent. Characteristically, mice are known to have a pointed snout, small rounded ears, a body-length scaly tail, and a high breeding rate. The best known mouse species is the common house mouse (''Mus musculus''). Mice are also popular as pets. In some places, certain kinds of Apodemus, field mice are locally common. They are known to invade homes for food and shelter. Mice are typically distinguished from rats by their size. Generally, when a muroid rodent is discovered, its common name includes the term ''mouse'' if it is smaller, or ''rat'' if it is larger. The common terms ''rat'' and ''mouse'' are not Taxonomy (biology), taxonomically specific. Typical mice are classified in the genus ''Mus (genus), Mus'', but the term ''mouse'' is not confined to members of ''Mus'' and can also apply to species from other genera such as the deer mouse, deer mouse (''Peromyscus''). Fancy mouse, Domestic mice sold as pets often differ substantially in size f ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Laser Scanning Microscopy
A laser is a device that emits light through a process of optical amplification based on the stimulated emission of electromagnetic radiation. The word ''laser'' originated as an acronym for light amplification by stimulated emission of radiation. The first laser was built in 1960 by Theodore Maiman at Hughes Research Laboratories, based on theoretical work by Charles H. Townes and Arthur Leonard Schawlow and the optical amplifier patented by Gordon Gould. A laser differs from other sources of light in that it emits light that is coherence (physics), ''coherent''. Spatial coherence allows a laser to be focused to a tight spot, enabling uses such as optical communication, laser cutting, and Photolithography#Light sources, lithography. It also allows a laser beam to stay narrow over great distances (collimated light, collimation), used in laser pointers, lidar, and free-space optical communication. Lasers can also have high temporal coherence, which permits them to emit light ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Excitation Cross Sections
Excitation, excite, exciting, or excitement may refer to: * Excitation (magnetic), provided with an electrical generator or alternator * ''Exite'', a series of racing video games published by Nintendo starting with ''Excitebike'' * Excite (web portal), web portal owned by IAC * Excite Ballpark, located in San Jose, California * Electron excitation, the transfer of an electron to a higher atomic orbital ** More generally, the transfer of energy to a normal mode * ''Excitement'' (film), a lost 1924 silent comedy by Robert F. Hill * Sexual excitation * Stimulation or excitation or excitement, the action of various agents on nerves, muscles, or a sensory end organ, by which activity is evoked * "Exciting", a song by Hieroglyphics from the album '' The Kitchen'' See also * Anticipation (emotion) * Anxiety * Endorphins * Excitatory postsynaptic potential * Excited (other) * Excited state, of an atom, molecule or nucleus * Exciter (other) * Pleasure * Psychomoto ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
