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Thayer–Martin Agar
Thayer–Martin agar (or Thayer–Martin medium, or VPN agar) is a Mueller–Hinton agar with 5% chocolate sheep blood and antibiotics. It is used for culturing and primarily isolating pathogenic ''Neisseria'' bacteria, including ''Neisseria gonorrhoeae'' and ''Neisseria meningitidis'', as the medium inhibits the growth of most other microorganisms. When growing ''Neisseria meningitidis'', one usually starts with a normally sterile body fluid (blood or CSF), so a plain chocolate agar is used. Thayer–Martin agar was initially developed in 1964, with an improved formulation published in 1966. Components It usually contains the following combination of antibiotics, which make up the VPN acronym: *Vancomycin, which is able to kill most Gram-positive organisms, although some Gram-positive organisms such as ''Lactobacillus'' and ''Pediococcus'' are intrinsically resistant *Colistin, Polymyxin, also known as colistin, which is added to kill most Gram-negative organisms except ''Neisse ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Neisseria Gonorrhoeae 01
''Neisseria'' is a large genus of bacteria that colonize the mucous membranes of many animals. Of the 11 species that colonize humans, only two are pathogens: ''Neisseria meningitidis, N. meningitidis'' and ''Neisseria gonorrhoeae, N. gonorrhoeae''. ''Neisseria'' species are Gram-negative bacteria included among the Pseudomonadota, a large group of Gram-negative forms. ''Neisseria'' Diplococcus, diplococci resemble coffee beans when viewed microscopically. Pathogenesis and classification Pathogens Species of this genus (family Neisseriaceae) of parasitic bacteria grow in pairs and occasionally fours, and thrive best at 98.6 °F (37 °C) in the animal body or serum media. The genus includes: * ''Neisseria gonorrhoeae, N. gonorrhoeae'' (also called the gonococcus) causes gonorrhea. * ''Neisseria meningitidis, N. meningitidis'' (also called the meningococcus) is one of the most common causes of bacterial meningitis and the causative agent of meningococcal septicaemia. T ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Pediococcus
''Pediococcus'' is a genus of gram-positive lactic acid bacteria, placed within the family of Lactobacillaceae. They usually occur in pairs or tetrads, and divide along two planes of symmetry, as do the other lactic acid cocci genera '' Aerococcus'' and '' Tetragenococcus''. They are purely homofermentative. Food processing ''Pediococcus'' is, along with other lactic acid bacteria such as '' Leuconostoc'' and ''Lactobacillus'', responsible for the fermentation of cabbage, making it sauerkraut. In this process, the sugars in fresh cabbage are fermented to lactic acid, which gives sauerkraut a sour flavour and good keeping qualities. ''Pediococcus'' bacteria are usually considered contaminants of beer and wine, although their presence is sometimes desired in beer styles such as lambic and Berliner Weisse. Certain ''Pediococcus'' isolates produce diacetyl which gives a buttery or butterscotch aroma to some wines (such as Chardonnay) and a few styles of beer. ''Pediococcus'' spe ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Pelvic Inflammatory Disease
Pelvic inflammatory disease (PID), also known as pelvic inflammatory disorder, is an infection of the upper part of the female reproductive system, mainly the uterus, fallopian tubes, and ovaries, and inside of the pelvis. Often, there may be no symptoms. Signs and symptoms, when present, may include lower abdominal pain, vaginal discharge, fever, dysuria, burning with urination, dyspareunia, pain with sex, postcoital bleeding, bleeding after sex, or irregular menstruation. Untreated PID can result in long-term complications including infertility, ectopic pregnancy, chronic pelvic pain, and Infectious causes of cancer, cancer. The disease is caused by bacteria that spread from the vagina and cervix. It has been reported that infections by ''Neisseria gonorrhoeae'' or ''Chlamydia trachomatis'' are present in 75 to 90 percent of cases. However, in the UK it is reported by the NHS that infections by ''Neisseria gonorrhoeae'' and ''Chlamydia trachomatis'' are responsible for only a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Trimethoprim
Trimethoprim (TMP) is an antibiotic used mainly in the treatment of bladder infections. Other uses include for middle ear infections and travelers' diarrhea. With sulfamethoxazole or dapsone it may be used for ''Pneumocystis'' pneumonia in people with HIV/AIDS. It is taken orally (swallowed by mouth). Common side effects include nausea, changes in taste, and rash. Rarely it may result in blood problems such as not enough platelets or white blood cells. Trimethoprim may cause sun sensitivity. There is evidence of potential harm during pregnancy in some animals but not humans. It works by blocking folate metabolism via dihydrofolate reductase in some bacteria, preventing creation of bacterial DNA and RNA and leading to bacterial cell death. Trimethoprim was first used in 1962. It is on the World Health Organization's List of Essential Medicines. It is available as a generic medication. Medical uses It is primarily used in the treatment of urinary tract infectio ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fungi
A fungus (: fungi , , , or ; or funguses) is any member of the group of eukaryotic organisms that includes microorganisms such as yeasts and mold (fungus), molds, as well as the more familiar mushrooms. These organisms are classified as one of the kingdom (biology)#Six kingdoms (1998), traditional eukaryotic kingdoms, along with Animalia, Plantae, and either Protista or Protozoa and Chromista. A characteristic that places fungi in a different kingdom from plants, bacteria, and some protists is chitin in their cell walls. Fungi, like animals, are heterotrophs; they acquire their food by absorbing dissolved molecules, typically by secreting digestive enzymes into their environment. Fungi do not photosynthesize. Growth is their means of motility, mobility, except for spores (a few of which are flagellated), which may travel through the air or water. Fungi are the principal decomposers in ecological systems. These and other differences place fungi in a single group of related o ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Nystatin
Nystatin, sold under the brand name Mycostatin among others, is an antifungal medication. It is used to treat ''Candida (fungus), Candida'' infections of the skin including diaper rash, Candidiasis, thrush, esophageal candidiasis, and vaginal yeast infections. It may also be used to prevent candidiasis in those who are at high risk. Nystatin may be used by mouth, in the vagina, or applied to the skin. Common side effects when applied to the skin include burning, itching, and a rash. Common side effects when taken by mouth include vomiting and diarrhea. During pregnancy use in the vagina is safe while other formulations have not been studied in this group. It works by disrupting the cell membrane of the fungal cells. Nystatin was discovered in 1950 by Rachel Fuller Brown and Elizabeth Lee Hazen. It was the first polyene macrolide antifungal. It is on the WHO Model List of Essential Medicines, World Health Organization's List of Essential Medicines. It is available as a generi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Legionella
''Legionella'' is a genus of gram-negative bacteria, gram-negative bacteria that can be seen using a silver stain or grown in a special media that contains cysteine, an amino acid. It is known to cause legionellosis (all illnesses caused by ''Legionella'') including a pneumonia-type illness called Legionnaires' disease and a mild flu-like illness called Pontiac fever. These bacteria are common in many places, like soil and water. There are over 50 species and 70 types (serotype, serogroups) identified. ''Legionella'' does not spread from person-to-person. Most individuals who are exposed to the bacteria do not get sick. Most outbreaks result from poorly maintained cooling towers. The cell wall of the ''Legionella'' bacteria has parts that determine its specific type. The structural arrangement and building blocks (sugars) in the cell wall help classify the bacteria. Etymology ''Legionella'' was named after 1976 Philadelphia Legionnaires' disease outbreak, a 1976 outbreak of a th ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gram-negative
Gram-negative bacteria are bacteria that, unlike gram-positive bacteria, do not retain the crystal violet stain used in the Gram staining method of bacterial differentiation. Their defining characteristic is that their cell envelope consists of a thin peptidoglycan cell wall sandwiched between an inner ( cytoplasmic) membrane and an outer membrane. These bacteria are found in all environments that support life on Earth. Within this category, notable species include the model organism '' Escherichia coli'', along with various pathogenic bacteria, such as '' Pseudomonas aeruginosa'', '' Chlamydia trachomatis'', and '' Yersinia pestis''. They pose significant challenges in the medical field due to their outer membrane, which acts as a protective barrier against numerous antibiotics (including penicillin), detergents that would normally damage the inner cell membrane, and the antimicrobial enzyme lysozyme produced by animals as part of their innate immune system. Furthe ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Colistin
Colistin, also known as polymyxin E, is an antibiotic medication used as a last-resort treatment for multidrug-resistant Gram-negative infections including pneumonia. These may involve bacteria such as ''Pseudomonas aeruginosa'', ''Klebsiella pneumoniae'', or ''Acinetobacter''. It comes in two forms: colistimethate sodium can be injected into a vein, injected into a muscle, or inhaled, and colistin sulfate is mainly applied to the skin or taken by mouth. Colistimethate sodium is a prodrug; it is produced by the reaction of colistin with formaldehyde and sodium bisulfite, which leads to the addition of a sulfomethyl group to the primary amines of colistin. Colistimethate sodium is less toxic than colistin when administered parenterally. In aqueous solutions, it undergoes hydrolysis to form a complex mixture of partially sulfomethylated derivatives, as well as colistin. Resistance to colistin began to appear as of 2015. Common side effects of the injectable form include kidne ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Lactobacillus
''Lactobacillus'' is a genus of gram-positive, aerotolerant anaerobes or microaerophilic, rod-shaped, non-spore-forming bacteria. Until 2020, the genus ''Lactobacillus'' comprised over 260 phylogenetically, ecologically, and metabolically diverse species; a taxonomic revision of the genus assigned lactobacilli to 25 genera (see below). ''Lactobacillus'' species constitute a significant component of the human and animal microbiota at a number of body sites, such as the digestive system and the female genital system. In women of European ancestry, ''Lactobacillus'' species are normally a major part of the vaginal microbiota. ''Lactobacillus'' forms biofilms in the vaginal and gut microbiota, allowing them to persist in harsh environmental conditions and maintain ample populations. ''Lactobacillus'' exhibits a mutualistic relationship with the human body, as it protects the host against potential invasions by pathogens, and in turn, the host provides a source of nutrients ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mueller–Hinton Agar
Mueller Hinton agar is a type of growth medium used in microbiology to culture bacterial isolates and test their susceptibility to antibiotics. This medium was first developed in 1941 by John Howard Mueller and Jane Hinton, who were microbiologists working at Harvard University. However, Mueller Hinton agar is made up of a couple of components, including beef extract, acid hydrolysate of casein, and starch, as well as agar to solidify the mixture. The composition of Mueller Hinton agar can vary depending on the manufacturer and the intended use, but the medium is generally nutrient-rich and free of inhibitors that could interfere with bacterial growth. Mueller Hinton agar is commonly used in the disk diffusion method, which is a simple and widely used method for testing the susceptibility of bacterial isolates to antibiotics. In this method, small disks impregnated with different antibioti ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gram-positive
In bacteriology, gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their type of cell wall. The Gram stain is used by microbiologists to place bacteria into two main categories, gram-positive (+) and gram-negative bacteria, gram-negative (−). Gram-positive bacteria have a thick layer of peptidoglycan within the cell wall, and gram-negative bacteria have a thin layer of peptidoglycan. Gram-positive bacteria retain the crystal violet stain used in the test, resulting in a purple color when observed through an optical microscope. The thick layer of peptidoglycan in the bacterial cell wall retains the Stain (biology), stain after it has been fixed in place by iodine. During the decolorization step, the decolorizer removes crystal violet from all other cells. Conversely, gram-negative bacteria cannot retain the violet stain after the decolorization ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
